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Commercial mushrooms are produced on lignocellulose such as straw, saw dust, and wood chips. As such, mushroom-forming fungi convert low-quality waste streams into high-quality food. Spent mushroom substrate (SMS) is usually considered a waste product. This review discusses the applications of SMS to promote the transition to a circular economy. SMS can be used as compost, as a substrate for other mushroom-forming fungi, as animal feed, to promote health of animals, and to produce packaging and construction materials, biofuels, and enzymes. This range of applications can make agricultural production more sustainable and efficient, especially if the CO2 emission and heat from mushroom cultivation can be used to promote plant growth in greenhouses.

Hydrophobins are proteins exclusively produced by filamentous fungi. They self-assemble at hydrophilic-hydrophobic interfaces into an amphipathic film. This protein film renders hydrophobic surfaces of gas bubbles, liquids, or solid materials wettable, while hydrophilic surfaces can be turned hydrophobic. These properties, among others, make hydrophobins of interest for medical and technical applications. For instance, hydrophobins can be used to disperse hydrophobic materials; to stabilize foam in food products; and to immobilize enzymes, peptides, antibodies, cells, and anorganic molecules on surfaces. At the same time, they may be used to prevent binding of molecules. Furthermore, hydrophobins have therapeutic value as immunomodulators and can been used to produce recombinant proteins.

Aureobasidium is omnipresent and can be isolated from air, water bodies, soil, wood, and other plant materials, as well as inorganic materials such as rocks and marble. A total of 32 species of this fungal genus have been identified at the level of DNA, of which Aureobasidium pullulans is best known. Aureobasidium is of interest for a sustainable economy because it can be used to produce a wide variety of compounds, including enzymes, polysaccharides, and biosurfactants. Moreover, it can be used to promote plant growth and protect wood and crops. To this end, Aureobasidium cells adhere to wood or plants by producing extracellular polysaccharides, thereby forming a biofilm. This biofilm provides a sustainable alternative to petrol-based coatings and toxic chemicals. This and the fact that Aureobasidium biofilms have the potential of self-repair make them a potential engineered living material avant la lettre. Key points • Aureobasidium produces products of interest to the industry • Aureobasidium can stimulate plant growth and protect crops • Biofinish of A. pullulans is a sustainable alternative to petrol-based coatings • Aureobasidium biofilms have the potential to function as engineered living materials

Efficient gene deletion methods are essential for the high-throughput study of gene function. Compared to most ascomycete model systems, gene deletion is more laborious in mushroom-forming basidiomycetes due to the relatively low incidence of homologous recombination (HR) and relatively high incidence of non-homologous end-joining (NHEJ). Here, we describe the use of pre-assembled Cas9-sgRNA ribonucleoproteins (RNPs) to efficiently delete the homeodomain transcription factor gene hom2 in the mushroom-forming basidiomycete Schizophyllum commune by replacing it with a selectable marker. All components (Cas9 protein, sgRNA, and repair template with selectable marker) were supplied to wild type protoplasts by PEG-mediated transformation, abolishing the need to optimize the expression of cas9 and sgRNAs. A Δku80 background further increased the efficiency of gene deletion. A repair template with homology arms of 250 bp was sufficient to efficiently induce homologous recombination. This is the first report of the use of pre-assembled Cas9 RNPs in a mushroom-forming basidiomycete and this approach may also improve the genetic accessibility of non-model species.

Geographical location is vital to geospatial applications like local search and event detection. In this paper, we investigate and improve on the task of text-based geolocation prediction of Twitter users. Previous studies on this topic have typically assumed that geographical references (e.g., gazetteer terms, dialectal words) in a text are indicative of its author’s location. However, these references are often buried in informal, ungrammatical, and multilingual data, and are therefore non-trivial to identify and exploit. We present an integrated geolocation prediction framework and investigate what factors impact on prediction accuracy. First, we evaluate a range of feature selection methods to obtain “location indicative words”. We then evaluate the impact of non-geotagged tweets, language, and user-declared metadata on geolocation prediction. In addition, we evaluate the impact of temporal variance on model generalisation, and discuss how users differ in terms of their geolocatability. We achieve state-of-the-art results for the text-based Twitter user geolocation task, and also provide the most extensive exploration of the task to date. Our findings provide valuable insights into the design of robust, practical text-based geolocation prediction systems.

SummaryThe incidence of atypical femoral fractures (AFFs) was 2.95% among 6644 hip and femoral fractures. Independent risk factors included the use of bisphosphonates (BPs), osteopenia or osteoporosis, rheumatoid arthritis, increased femoral curvatures, and thicker femoral cortices. Patients with AFFs and BP treatment were more likely to have problematic healing than those with typical femoral fractures (TFFs) and no BP treatment.IntroductionTo determine the incidence and risk factors of atypical femoral fractures (AFFs), we performed a multicenter case-control study. We also investigated the effects of bisphosphonates (BPs) on AFF healing.MethodsWe retrospectively reviewed the medical records and radiographs of 6644 hip and femoral fractures of patients from eight tertiary referral hospitals. All the radiographs were reviewed to distinguish AFFs from TFFs. Univariate and multivariate logistic regression analyses were performed to identify risk factors, and interaction analyses were used to investigate the effects of BPs on fracture healing.ResultsThe incidence of AFFs among 6644 hip and femoral fractures was 2.95% (90 subtrochanter and 106 femoral shaft fractures). All patients were females with a mean age of 72 years, and 75.5% were exposed to BPs for an average duration of 5.2 years (range, 1–17 years). The use of BPs was significantly associated with AFFs (p < 0.001, odds ratio = 25.65; 95% confidence interval = 10.74–61.28). Other independent risk factors for AFFs included osteopenia or osteoporosis, rheumatoid arthritis, increased anterior and lateral femoral curvatures, and thicker lateral femoral cortex at the shaft level. Interaction analyses showed that patients with AFFs using BPs had a significantly higher risk of problematic fracture healing than those with TFFs and no BP treatment.ConclusionsThe incidence of AFFs among 6644 hip and femoral fractures was 2.95%. Osteopenia or osteoporosis, use of BPs, rheumatoid arthritis, increased anterior and lateral femoral curvatures, and thicker lateral femoral cortex were independent risk factors for the development of AFFs. Patients with AFFs and BP treatment were more likely to have problematic fracture healing than those with TFFs and no BP treatment.

Filamentous fungi colonize substrates by forming a mycelium. This network of hyphae can be used as a bio-based material. Here, we assessed the impact of environmental growth conditions and deletion of the hydrophobin gene sc3 on material properties of the mycelium of the mushroom forming fungus Schizophyllum commune . Thermogravimetric analysis showed that Δ sc3 mycelium retained more water with increasing temperature when compared to the wild type. The Young’s modulus (E) of the mycelium ranged between 438 and 913 MPa when the wild type strain was grown in the dark or in the light at low or high CO 2 levels. This was accompanied by a maximum tensile strength (σ) of 5.1–9.6 MPa. In contrast, E and σ of the Δ sc3 strain were 3–4- fold higher with values of 1237–2727 MPa and 15.6–40.4 MPa, respectively. These values correlated with mycelium density, while no differences in chemical composition of the mycelia were observed as shown by ATR-FTIR. Together, genetic modification and environmental growth conditions impact mechanical properties of the mycelium by affecting the density of the mycelium. As a result, mechanical properties of wild type mycelium were similar to those of natural materials, while those of Δ sc3 were more similar to thermoplastics.

Excitons with binding energies of a few hundreds of meV control the optical properties of transition metal dichalcogenide monolayers. Knowledge of the fine structure of these excitons is therefore essential to understand the optoelectronic properties of these 2D materials. Here we measure the exciton fine structure of MoS 2 and MoSe 2 monolayers encapsulated in boron nitride by magneto-photoluminescence spectroscopy in magnetic fields up to 30 T. The experiments performed in transverse magnetic field reveal a brightening of the spin-forbidden dark excitons in MoS 2 monolayer: we find that the dark excitons appear at 14 meV below the bright ones. Measurements performed in tilted magnetic field provide a conceivable description of the neutral exciton fine structure. The experimental results are in agreement with a model taking into account the effect of the exchange interaction on both the bright and dark exciton states as well as the interaction with the magnetic field. Excitons control the optical properties of transition metal dichalcogenide monolayers. Here, the authors measure the exciton fine structure of MoS 2 and MoSe 2 monolayers encapsulated in hBN in magnetic fields up to 30 T, and observe a brightening of the spin-forbidden dark excitons in MoS 2 .

Growing colonies of the split-gill fungus Schizophyllum commune show action potential-like spikes of extracellular electrical potential. We analysed several days of electrical activity recording of the fungus and discovered three families of oscillatory patterns. Very slow activity at a scale of hours, slow activity at a scale of 10 min and very fast activity at scale of half-minute. We simulated the spiking behaviour using FitzHugh–Nagume model, uncovered mechanisms of spike shaping. We speculated that spikes of electrical potential might be associated with transportation of nutrients and metabolites.

Filamentous fungi colonise substrates by invasive growth of multi-cellular hyphae. It is commonly accepted that hyphae expand by tip growth that is restricted to the first apical cell, where turgor pressure, exocytosis and endocytosis cooperate to expand the apex. Here we show that, contrary to expectations, subapical cells play important roles in hyphal growth in the industrial enzyme-producing fungus Trichoderma reesei . We find that the second and third cells are crucial for hyphal extension, which correlates with tip-ward cytoplasmic streaming, and the fourth-to-sixth cells support rapid growth rates. Live cell imaging reveals exocytotic and endocytic activity in both apical and subapical cells, associated with microtubule-based bi-directional transport of secretory vesicles and early endosomes across septa. Moreover, visualisation of 1,3-β-glucan synthase in subapical cells reveals that these compartments deliver cell wall-forming enzymes to the apical growth region. Thus, subapical cells are active in exocytosis and endocytosis, and deliver growth supplies and enzymes to the expanding hyphal apex. It is commonly accepted that the hyphae of filamentous fungi expand by tip growth that is restricted to the first apical cell. Here, Schuster et al. show that, contrary to expectations, subapical cells play important roles in hyphal growth in the industrial enzyme-producing fungus Trichoderma reesei .