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"Huang, Ling"
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And I think about you
\"A wonderfully heartwarming look at what happens when a parent and child are separated during the workday, and the sweet moment when they're reunited at the end of the day.\"-- Provided by publisher.
Book
Enzymatic enhancing of triplet–triplet annihilation upconversion by breaking oxygen quenching for background-free biological sensing
Triplet-triplet annihilation upconversion nanoparticles have attracted considerable interest due to their promises in organic chemistry, solar energy harvesting and several biological applications. However, triplet-triplet annihilation upconversion in aqueous solutions is challenging due to sensitivity to oxygen, hindering its biological applications under ambient atmosphere. Herein, we report a simple enzymatic strategy to overcome oxygen-induced triplet-triplet annihilation upconversion quenching. This strategy stems from a glucose oxidase catalyzed glucose oxidation reaction, which enables rapid oxygen depletion to turn on upconversion in the aqueous solution. Furthermore, self-standing upconversion biological sensors of such nanoparticles are developed to detect glucose and measure the activity of enzymes related to glucose metabolism in a highly specific, sensitive and background-free manner. This study not only overcomes the key roadblock for applications of triplet-triplet annihilation upconversion nanoparticles in aqueous solutions, it also establishes the proof-of-concept to develop triplet-triplet annihilation upconversion nanoparticles as background free self-standing biological sensors.
Triplet-triplet annihilation upconversion (TTA-UC) nanoparticles (NPs) are a promising material for diverse applications, but TTA-UC is sensitive to oxygen, which hinders its biological utility. Here, the authors report an enzymatic method to deplete oxygen and amplify the upconversion of TTA-UCNP in aqueous solution, and develop background-free glucose and glucose metabolism enzyme sensors.
Journal Article
Expression in Antennae and Reproductive Organs Suggests a Dual Role of an Odorant-Binding Protein in Two Sibling Helicoverpa Species
Odorant-binding proteins (OBPs) mediate both perception and release of semiochemicals in insects. These proteins are the ideal targets for understanding the olfactory code of insects as well as for interfering with their communication system in order to control pest species. The two sibling Lepidopteran species Helicoverpa armigera and H. assulta are two major agricultural pests. As part of our aim to characterize the OBP repertoire of these two species, here we focus our attention on a member of this family, OBP10, particularly interesting for its expression pattern. The protein is specifically expressed in the antennae of both sexes, being absent from other sensory organs. However, it is highly abundant in seminal fluid, is transferred to females during mating and is eventually found on the surface of fertilised eggs. Among the several different volatile compounds present in reproductive organs, OBP10 binds 1-dodecene, a compound reported as an insect repellent. These results have been verified in both H. armigera and H. assulta with no apparent differences between the two species. The recombinant OBP10 binds, besides 1-dodecene, some linear alcohols and several aromatic compounds. The structural similarity of OBP10 with OBP1 of the mosquito Culex quinquefasciatus, a protein reported to bind an oviposition pheromone, and its affinity with 1-dodecene suggest that OBP10 could be a carrier for oviposition deterrents, favouring spreading of the eggs in these species where cannibalism is active among larvae.
Journal Article
Predictors of caregiver burden in aged caregivers of demented older patients
Background
Dementia in the oldest-old is projected to increase exponentially as is the burden of their caregivers who may experience unique challenges and suffering. Thus, we aim to investigate which factors are associated with older caregivers’ burden in caring demented outpatients in a multicenter cohort.
Methods
Patients and their caregivers, both aged ≧65 years, in the National Dementia Registry Study in Taiwan (T-NDRS) were included in this study. Caregiver burden was measured with the short version of the Zarit Burden Interview (ZBI). The correlations between the ZBI scores and characteristics of caregivers and patients, including severity of dementia, physical comorbidities, instrumental activities of daily living (IADL), neuropsychiatric symptoms assessed by the Neuropsychiatric Inventory (NPI), and family monthly income, were analyzed.
Results
We recruited 328 aged informal caregiver-patient dyads. The mean age of caregivers was 73.7 ± 7.0 years, with female predominance (66.8%), and the mean age of patients was 78.8 ± 6.9 years, with male predominance (61.0%). Multivariable linear regression showed that IADLs (β = 0.83,
p
< 0.001) and NPI subscores of apathy (β = 3.83,
p
< 0.001)and irritability (β = 4.25,
p
< 0.001) were positively associated with ZBI scores. The highest family monthly income (β = − 10.92,
p
= 0.001) and caregiver age (β = − 0.41,
p
= 0.001) were negatively correlated with ZBI scores.
Conclusions
Older caregivers of older demented patients experience a higher care burden when patients had greater impaired functional autonomy and the presence of NPI symptoms of apathy and irritability. Our findings provide the direction to identify risky older caregivers, and we should pay more attention to and provide support for these exhausted caregivers.
Journal Article
Nano-drug delivery systems in wound treatment and skin regeneration
Skin damages are defined as one of most common lesions people suffer from, some of wounds are notoriously difficult to eradicate such as chronic wounds and deep burns. Existing wound therapies have been proved to be inadequate and far from satisfactory. The cutting-edge nanotechnology offers an unprecedented opportunity to revolutionize and invent new therapies or boost the effectiveness of current medical treatments. In particular, the nano-drug delivery systems anchor bioactive molecules to applied area, sustain the drug release and explicitly enhance the therapeutic efficacies of drugs, thus making a fine figure in field relevant to skin regeneration. This review summarized and discussed the current nano-drug delivery systems holding pivotal potential for wound healing and skin regeneration, with a special emphasis on liposomes, polymeric nanoparticles, inorganic nanoparticles, lipid nanoparticles, nanofibrous structures and nanohydrogel.
Journal Article
Generation and expansion of highly pure motor neuron progenitors from human pluripotent stem cells
Human pluripotent stem cells (hPSCs) have opened new opportunities for understanding human development, modelling disease processes and developing new therapeutics. However, these applications are hindered by the low efficiency and heterogeneity of cell types, such as motorneurons (MNs), differentiated from hPSCs as well as our inability to maintain the potency of lineage-committed progenitors. Here by using a combination of small molecules that regulate multiple signalling pathways, we develop a method to guide human embryonic stem cells to a near-pure population (>95%) of motor neuron progenitors (MNPs) in 12 days, and an enriched population (>90%) of functionally mature MNs in an additional 16 days. More importantly, the MNPs can be expanded for at least five passages so that a single MNP can be amplified to 1 × 10
4
. This method is reproducible in human-induced pluripotent stem cells and is applied to model MN-degenerative diseases and in proof-of-principle drug-screening assays.
Applications of human pluripotent stem cells (hPSCs) for disease modelling or cell therapy are hindered by low efficiency and heterogeneity of target cell types differentiated from hPSCs, such as motor neurons (MNs). Here the authors develop a method to derive highly pure motor neuron progenitor populations from human embryonic and induced pluripotent stem cells that yield functional MNs.
Journal Article
Bibliometric Analysis on Smart Cities Research
Smart cities have been a global concern in recent years, involving comprehensive scientific research. To obtain a structural overview and assist researchers in making insights into the characteristics of smart cities research, bibliometric analysis was carried out in this paper. With the application of the bibliometric analysis software VOSviewer and CiteSpace, 4409 smart cities were identified by the core collection of the Web of Science in publications between 1998 and 2019 and used in the analysis of this paper. Concretely, this research visually demonstrates a comprehensive overview of the field relating to smart cities in terms of the production of regular publications, main domain of smart cities researchers, most influential countries (institutions, sources and authors), and interesting research directions in the smart city researches. We also present the research collaboration among countries (regions), organizations and authors based on a series of cooperation analyses. The bibliometric analysis of the existing work provided a valuable and seminal reference for researchers and practitioners in smart cities-related research communities.
Journal Article
Engineered extracellular vesicles carrying let-7a-5p for alleviating inflammation in acute lung injury
Background
Acute lung injury (ALI) is a life-threatening respiratory condition characterized by severe inflammation and lung tissue damage, frequently causing rapid respiratory failure and long-term complications. The microRNA
let-7a-5p
is involved in the progression of lung injury, inflammation, and fibrosis by regulating immune cell activation and cytokine production. This study aims to use an innovative cellular electroporation platform to generate extracellular vesicles (EVs) carring
let-7a-5p
(EV-
let-7a-5p
) derived from transfected Wharton’s jelly-mesenchymal stem cells (WJ-MSCs) as a potential gene therapy for ALI.
Methods
A cellular nanoporation (CNP) method was used to induce the production and release of EV-
let-7a-5p
from WJ-MSCs transfected with the relevant plasmid DNA. EV-
let-7a-5p
in the conditioned medium were isolated using a tangential flow filtration (TFF) system. EV characterization followed the minimal consensus guidelines outlined by the International Society for Extracellular Vesicles. We conducted a thorough set of therapeutic assessments, including the antifibrotic effects using a transforming growth factor beta (TGF-β)-induced cell model, the modulation effects on macrophage polarization, and the influence of EV-
let-7a-5p
in a rat model of hyperoxia-induced ALI.
Results
The CNP platform significantly increased EV secretion from transfected WJ-MSCs, and the encapsulated
let-7a-5p
in engineered EVs was markedly higher than that in untreated WJ-MSCs. These EV-
let-7a-5p
did not influence cell proliferation and effectively mitigated the TGF-β-induced fibrotic phenotype by downregulating SMAD2/3 phosphorylation in LL29 cells. Furthermore, EV-
let-7a-5p
regulated M2-like macrophage activation in an inflammatory microenvironment and significantly induced interleukin (IL)-10 secretion, demonstrating their modulatory effect on inflammation. Administering EVs from untreated WJ-MSCs slightly improved lung function and increased
let-7a-5p
expression in plasma in the hyperoxia-induced ALI rat model. In comparison, EV-
let-7a-5p
significantly reduced macrophage infiltration and collagen deposition while increasing IL-10 expression, causing a substantial improvement in lung function.
Conclusion
This study reveals that the use of the CNP platform to stimulate and transfect WJ-MSCs could generate an abundance of
let-7a-5p
-enriched EVs, which underscores the therapeutic potential in countering inflammatory responses, fibrotic activation, and hyperoxia-induced lung injury. These results provide potential avenues for developing innovative therapeutic approaches for more effective interventions in ALI.
Journal Article