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"Irshad, Ahmad"
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CRISPR/Cas9—A Promising Therapeutic Tool to Cure Blindness: Current Scenario and Future Prospects
CRISPR-based targeted genome editing is bringing revolutionary changes in the research arena of biological sciences. CRISPR/Cas9 has been explored as an efficient therapeutic tool for the treatment of genetic diseases. It has been widely used in ophthalmology research by using mouse models to correct pathogenic mutations in the eye stem cells. In recent studies, CRISPR/Cas9 has been used to correct a large number of mutations related to inherited retinal disorders. In vivo therapeutic advantages for retinal diseases have been successfully achieved in some rodents. Current advances in the CRISPR-based gene-editing domain, such as modified Cas variants and delivery approaches have optimized its application to treat blindness. In this review, recent progress and challenges of the CRISPR-Cas system have been discussed to cure blindness and its prospects.
Journal Article
Latest Developed Strategies to Minimize the Off-Target Effects in CRISPR-Cas-Mediated Genome Editing
Gene editing that makes target gene modification in the genome by deletion or addition has revolutionized the era of biomedicine. Clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 emerged as a substantial tool due to its simplicity in use, less cost and extraordinary efficiency than the conventional gene-editing tools, including zinc finger nucleases (ZFNs) and Transcription activator-like effector nucleases (TALENs). However, potential off-target activities are crucial shortcomings in the CRISPR system. Numerous types of approaches have been developed to reduce off-target effects. Here, we review several latest approaches to reduce the off-target effects, including biased or unbiased off-target detection, cytosine or adenine base editors, prime editing, dCas9, Cas9 paired nickase, ribonucleoprotein (RNP) delivery and truncated gRNAs. This review article provides extensive information to cautiously interpret off-target effects to assist the basic and clinical applications in biomedicine.
Journal Article
Biosynthesis of Metal Nanoparticles via Microbial Enzymes: A Mechanistic Approach
During the last decade, metal nanoparticles (MtNPs) have gained immense popularity due to their characteristic physicochemical properties, as well as containing antimicrobial, anti-cancer, catalyzing, optical, electronic and magnetic properties. Primarily, these MtNPs have been synthesized through different physical and chemical methods. However, these conventional methods have various drawbacks, such as high energy consumption, high cost and the involvement of toxic chemical substances. Microbial flora has provided an alternative platform for the biological synthesis of MtNPs in an eco-friendly and cost effective way. In this article we have focused on various microorganisms used for the synthesis of different MtNPs. We also have elaborated on the intracellular and extracellular mechanisms of MtNP synthesis in microorganisms, and have highlighted their advantages along with their challenges. Moreover, due to several advantages over chemically synthesized nanoparticles, the microbial MtNPs, with their exclusive and dynamic characteristics, can be used in different sectors like the agriculture, medicine, cosmetics and biotechnology industries in the near future.
Journal Article
Multiple biomarker responses (serum biochemistry, oxidative stress, genotoxicity and histopathology) in Channa punctatus exposed to heavy metal loaded waste water
Experiments were conducted to investigate the health of fish
Channa punctatus
inhabiting heavy metal-loaded waste water. Heavy metals in the order of Fe > Mn > Zn > Co > Ni > Cu = Cr were present in the waste water. Gills had high metal load followed by liver and then kidney. Albumin, albumin to globulin (A:G) ratio, triglyceride, high density lipoprotein (HDL) and very low density lipoprotein (VLDL) were found to be lower but phospholipid, low density lipoprotein (LDL), total protein, lipid and cholesterol were higher as compared to the reference. Oxidative stress markers such as superoxide dismutase (SOD), catalase (CAT), glutathione S transferase (GST) and lipid peroxidation (LPO) were significantly higher in all tissues, whereas reduced glutathione (GSH) levels were comparatively low. Damage to DNA was observed with significantly higher mean tail length of comets in the exposed fish gill cells (30.9 µm) followed by liver (24.3 µm) and kidney (20.6 µm) as compared to reference fish (5.2, 4.8 and 5.9 µm respectively). Histopathology in gill, liver and kidney also showed marked damage. Integrated biochemical, oxidative stress, genotoxicity and histopathological findings are valuable biomarkers for native fish adaptive patterns, and monitoring of water quality/pollution of freshwater ecosystems.
Journal Article
Potential of Microalgae as Feed Supplements for Sustainable Aquaculture
ABSTRACT Aquaculture is a fast-growing industry mainly depends on the key feedstuffs, fishmeal (FM) and fish oil (FO) that will be limited with the passage of time due to the insubstantial resources available for wild fish harvesting. Therefore, other sources of feedstuffs need to be investigated to substitute FM and FO in aquafeeds. Terrestrial crops can be used to substitutes a portion of the FM however; they can result in changes in the nutritional quality of the fish produced. Microalgae can be considered as a favorable alternative that can substitute FM and FO ensuring the principles of sustainability in aquaculture. Microalgae are reasonably rich in proteins, lipids, carbohydrates, vitamins, minerals, pigments, etc., which are essential for not only sustaining fish health but also its unique array of bioactive compounds can improve coloration and quality of fillet. The aim of this review is to provide an update of the current knowledge of microalgae as a supplement or feed additive to substitute FM and FO in aquafeeds. This review will provide a platform to highlight the potential of microalgae-based aquafeeds for a sustainable aquaculture industry.
Journal Article
Exogenous melatonin confers drought stress by promoting plant growth, photosynthetic capacity and antioxidant defense system of maize seedlings
Melatonin is an important biologically active hormone that plays a vital role in plant growth and development. In particular, it has been investigated for its roles in abiotic stress management. The current experiment was carried out to investigate the protective role of melatonin in photosynthetic traits and the antioxidant defense system of maize seedling under drought stress. Maize seedlings were subjected to drought stress (40–45% FC) after two weeks of seedling emergence, followed by a foliar spray (0, 25, 50, 75 and 100 µM) and soil drench of melatonin (0, 25, 50, 75 and 100 µM). Our results indicated that drought stress negatively affected maize seedling and decreased plant growth and development, biomass accumulation, reduced chlorophyll, and carotenoid content, and significantly declined photosynthetic rate and stomatal conductance. On the other hand, reactive oxygen species, soluble protein, and proline content increased under drought stress. However, the application of exogenous melatonin reduced the reactive oxygen species burst and enhanced the photosynthetic activity by protecting from damages through activation of various antioxidant enzymes under drought stress. Foliar application of 100 µM and soil drench of 50 µM melatonin was the most effective treatment concentrations under drought stress. Our current findings hereby confirmed the mitigating potential of melatonin application for drought stress by maintaining plant growth, improving the photosynthetic characteristics and activities of antioxidants enzymes.
Journal Article
Prediction of Pile Bearing Capacity Using XGBoost Algorithm: Modeling and Performance Evaluation
The major criteria that control pile foundation design is pile bearing capacity (Pu). The load bearing capacity of piles is affected by the various characteristics of soils and the involvement of multiple parameters related to both soil and foundation. In this study, a new model for predicting bearing capacity is developed using an extreme gradient boosting (XGBoost) algorithm. A total of 200 driven piles static load test-based case histories were used to construct and verify the model. The developed XGBoost model results were compared to a number of commonly used algorithms—Adaptive Boosting (AdaBoost), Random Forest (RF), Decision Tree (DT) and Support Vector Machine (SVM) using various performance measure metrics such as coefficient of determination, mean absolute error, root mean square error, mean absolute relative error, Nash–Sutcliffe model efficiency coefficient and relative strength ratio. Furthermore, sensitivity analysis was performed to determine the effect of input parameters on Pu. The results show that all of the developed models were capable of making accurate predictions however the XGBoost algorithm surpasses others, followed by AdaBoost, RF, DT, and SVM. The sensitivity analysis result shows that the SPT blow count along the pile shaft has the greatest effect on the Pu.
Journal Article
Integrated Pest Management of Rhynchophorus ferrugineus Olivier: An Efficient Approach to Reduce Infestation in Date Palm Trees
ABSTRACT Date palm (Phoenix dactylifera L) is an essential fruit crop that is widely grown since ancient times in the desert areas of the world comprising the Southwest Asia, North Africa besides Mexico, Australia, South America, and United States. A number of insect pests including red palm weevil (Rhynchophorus ferrugineus Olivier) that is a devastating insect attacking palm trees. It is very difficult to control as its entire lifecycle is hidden inside the host trunk. To overcome this pest, integrated pest management program (IPM) strategy can be adopted that includes visual inspection, behavior management through pheromones, sanitation of the crop and field, removal of the infested frond and offshoot, eradicating the concealed breeding sites of the pest. IPM also include cultural techniques comprising in-groove humidity and palm density, judicious use of insecticides, using biological control agents including entomopathogenic fungi, nematodes as well as adopting the regulatory methods of domestic and international quarantine. This paper provides updated information about IPM, which look like a promising paradigm that can be adopted for the control of red palm weevil.
Journal Article
Heavy metal pollution and risk assessment by the battery of toxicity tests
The current study was carried out on dominant fish
Oreochromis niloticus
and water collected from the polluted Yamuna River, Agra, India. The heavy metals in water, recorded as follows: Fe > Mn > Zn > Cu > Ni > Cr > Cd and all were found to be above the prescribed limits. According to metal pollution index, exposed muscle (49.86), kidney (47.68) and liver (45.26) have been recorded to have higher bioaccumulation. The blood biochemical analysis of exposed
O. niloticus
indicated significant increase in activities of aspartate aminotransferase (+ 343.5%), alkaline phosphatase (+ 673.6%), alanine aminotransferase (+ 309.1%), and creatinine (+ 494.3%) over the reference. However, a significant decrease in albumin (A): globulins (G) ratio (− 87.86%) was observed. Similarly, the exposed fish also showed significant increase in total leucocyte count (+ 121%), differential leucocyte count, respiratory burst (+ 1175%), and nitric oxide synthase (+ 420%). The histological examination of liver and kidney showed tissue injury. Moreover, micronuclei (0.95%), kidney shaped nuclei (1.2%), and lobed nuclei (0.6%) along with DNA damage in the form of mean tail length in the liver (20.7 µm) and kidney (16.5 µm) was observed in the exposed
O. niloticus
. Potential health risk assessments based on estimated daily intake, target hazard quotient, hazard index, and target cancer risk indicated health risks associated with the consumption of these contaminated fishes. In conclusion, the present study showed that exposure to heavy metals contaminated water can alter immunological response; induce histopathological alterations and DNA damage in the studied fish. The consumption of this contaminated water or fish could have serious impact on human health.
Journal Article