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There is a relatively simple gas reservoir structure in gas storage A, with good trap sealing, large cap rock thickness, relatively inactive fault activity, good overall reservoir connectivity, large storage capacity, water invasion occurs in only a few wells in the low part of the structure, so there are the geological conditions for building gas storage reservoir. This paper analyzed the capacity and maximum recovery capacity of horizontal wells in this block, and the pressure influence range during the recovery process of high-speed pilot production through the pilot test of reservoir construction; moreover, it got the following understanding: the horizontal wells with good development effect, high recovery capacity, and the pressure influence range in this block are mainly within 400m from the well distance; there are differences in the gas production capacity of different types of reservoirs, and the capacity contribution of Class I reservoirs is large, while the capacity contribution of Class II and III reservoirs is small, together these believe that Shengping gas storage has the capacity conditions for building reservoir.

Non-alcoholic fatty liver disease (NAFLD) is a prevalent condition strongly linked to obesity, diabetes, and metabolic syndrome. Its global incidence is steadily increasing, placing a significant health burden on both patients and society. This study aims to identify ferroptosis-related differentially expressed genes (DEGs) through bioinformatics analysis and to explore their roles in NAFLD. By comparing samples from NAFLD patients and healthy controls, we identified 1,770 significant DEGs, with 1,073 being upregulated and 697 downregulated. Pathway analysis revealed a marked decrease in expression within certain key metabolic pathways (such as the one-carbon pool by folate) in the NAFLD group, while expression in DNA repair-related pathways (such as non-homologous end joining) was significantly increased. Additionally, immune cell infiltration analysis showed significant differences in 19 immune cell types between the NAFLD and control groups, with 12 types exhibiting increased infiltration in the NAFLD group. Through protein-protein interaction (PPI) network analysis, we identified 41 critical intersecting genes, and ROC curve validation demonstrated that 25 of these genes had an AUC value exceeding 0.85, highlighting their potential as biomarkers for NAFLD in early diagnosis and personalized treatment in the future.This study identifies critical ferroptosis-related genes and immune cell infiltration differences in NAFLD, offering potential biomarkers for early diagnosis and personalized treatment.

Carbon (C) plays a vital role in regulating soil nutrient cycling and increasing soil microbial community, but there is still limited information on how C source utilization characteristics responds to soil physical and chemical properties changes under double-cropping rice ( Oryza sativa L.) paddy field in southern China. Therefore, the effects of different short-term manure nitrogen (N) input rate managements on C source utilization characteristics in rice rhizosphere and non-rhizosphere soils under double-cropping rice field in southern China were studied by using 18 O-H 2 O method. Therefore, a field experiment were established in Ningxiang city of Hunan Province, and five different fertilizer treatments were applied: (1) 100% N of chemical fertilizer (M0), (2) 30% N of organic manure and 70% N of chemical fertilizer (M30), (3) 50% N of organic manure and 50% N of chemical fertilizer (M50), (4) 100% N of organic manure (M100), and (5) without N fertilizer input as control (CK). The results showed that soil microbial biomass C content, soil microbial growth rate, and soil microbial basal respiration with application of organic manure treatments (M30, M50, M100) were significantly higher ( p  < 0.05) than that of CK treatment. And the soil C utilization efficiency with M0 treatment were significantly higher ( p  < 0.05) than that of M100 treatment. Compared with CK and M0 treatments, the metabolic capacity of soil microorganisms to exogenous C sources with M30, M50 and M100 treatments were increased. The largest types of exogenous C source was carboxylic acids, followed by amino acid and carbohydrate, and complex compounds was the smallest. The RDA analysis results indicated that fertilizer treatments significantly changed the utilization characteristics of soil microorganisms to exogenous C sources. As a result, this study found that characteristics of soil C source utilization were significantly affected by different short-term manure N input rate managements.

Soil organic carbon (SOC) plays a vital role in maintaining or enhancing soil fertility and quality of paddy field, but there is still limited information about how SOC mineralization responds to different tillage managements under the double-cropping rice ( Oryza sativa L.) system in southern of China. Therefore, this study was designed to explore the changes in SOC content, soil enzyme activities (invertase, cellulose and urease), SOC mineralization at 0–10 cm and 10–20 cm layers and its relationship with 7-years tillage management under the double-cropping rice system of southern China. The experiment included four tillage managements: rotary tillage with all residues removed as a control (RTO), conventional tillage with residue incorporation (CT), rotary tillage with residue incorporation (RT), and no-tillage with residue retention (NT). The results indicated that SOC and soil labile organic carbon contents at 0–10 cm and 10–20 cm layers in paddy field with CT and RT treatments were significantly higher than the RTO treatment. Compared to the RTO treatment, SOC mineralization and accumulation at 0–10 cm and 10–20 cm layers in paddy field with CT, RT and NT treatments were increased. SOC accumulation and potential mineralization at 0–10 cm layer with NT treatment were significantly higher than the CT, RT and RTO treatments. Soil mineralization constant at 10–20 cm layer with CT treatment was significantly higher than those of RT, NT and RTO treatments. This result indicated SOC mineralization rate and accumulation at 10–20 cm layer of CT, RT, NT and RTO treatments were lower than those of treatments at 0–10 cm layer. Compared to RTO treatment, soil invertase, cellulose and urease activities with CT and RT treatments were significantly increased. Compared to RTO treatment, soil invertase, cellulose and urease activities at 0–20 cm layer of CT treatment increased by 22.6%, 46.2% and 89.0%, respectively. There was significantly positive correlation between SOC accumulation and SOC content, soil invertase, cellulose, urease activities, but SOC accumulation was significantly negative correlated with soil pH, bulk density. Therefore, CT and RT treatments were beneficial managements to improve SOC content and SOC mineralization in the double-cropping rice field of southern China.

Ammonia oxidation plays a vital role in regulating soil nitrogen (N) cycle in agricultural soil, which is significantly influenced by different fertilizer regimes. However, there is still need to further investigate the effects of different fertilizer managements on rhizosphere soil ammonia-oxidizing archaea (AOA) and bacteria (AOB) community in the double-cropping rice field. Therefore, the effects of different long-term (37 years) fertilizer managements on rhizosphere soil potential nitrification activity (PNA), AOA and AOB community structure, and its relationship under the double-cropping rice system in southern of China were studied in the present paper. The field experiment included without fertilizer input as a control (CK), inorganic fertilizer (MF), rice straw and inorganic fertilizer (RF), 30% organic manure and 70% inorganic fertilizer (OM). This result indicated that rhizosphere soil organic carbon (SOC) (SOC), total N and ammonium N (NH 4 + -N) contents in paddy field with RF and OM treatments were increased. Rhizosphere soil PNA, potential nitrification rate (PNR) and abundance of AOB in paddy field with MF treatment were increased, and abundance of AOA in paddy field with RF and OM treatments were increased, respectively. The result also showed that rhizosphere soil diversity index of AOA and AOB with RF and OM treatments were enhanced, compared with CK treatment. Rhizosphere soil AOB and AOA community composition was dominated by Proteobacteria , Actinobacteria and Acidobacteria with all fertilizer treatments. There had significantly positively correlation between the abundance of AOA and SOC, total N, and NH 4 -N contents. However, there had significantly negatively correlation between soil pH and abundance of AOA, soil PNA, PNR. As a result, long-term application of rice straw and organic manure was benefit for increasing community structure of rhizosphere soil ammonia oxidizer under the double-cropping rice system in southern of China.

Steroid-induced osteonecrosis of the femoral head (SONFH) is a debilitating condition caused by long-term corticosteroid use, leading to impaired blood flow and bone cell death. The disruption of cellular processes and promotion of apoptosis by endoplasmic reticulum stress (ERS) is implicated in the pathogenesis of SONFH. We identified ERS-associated genes in SONFH and investigated their potential as therapeutic targets. We analysed the GSE123568 GEO dataset to identify differentially expressed genes (DEGs) related to ERS in SONFH. We conducted Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses, identified hub genes by protein-protein interaction (PPI) analyses, and evaluated their functions by gene set enrichment analysis (GSEA). We constructed mRNA–miRNA networks, identified potential therapeutics, and assessed immune cell infiltration. We performed cross-validation using the GEO dataset GSE74089, qRT-PCR on clinical samples from patients with SONFH and controls, and a receiver operating characteristic (ROC) curve analysis to assess the diagnostic performance of the hub genes. We identified 195 ERS-related genes in SONFH, which were primarily involved in oxidative stress, immune responses, and metabolic pathways. The PPI network suggested CXCL8, STAT3, IL1B, TLR4, PTGS2, TLR2, CASP1, CYBB, CAT, and HOMX1 to be key hub genes, which were shown by GSEA to be involved in biological pathways related to metabolism, immune modulation, and cellular integrity. We also identified 261 microRNAs (miRNAs) as well as drugs such as dibenziodolium and N -acetyl-L-cysteine that modulated inflammatory responses in SONFH. Twenty-two immune cell subtypes showed significant correlations, such as a positive correlation between activated mast cells and Tregs, and patients with SONFH had fewer dendritic cells than controls. The hub genes CYBB and TLR4 showed significant correlations with M1 macrophages and CD8 T cells, respectively. Cross-validation and qRT-PCR confirmed the upregulation of STAT3, IL1B, TLR2, and CASP1 in patients with SONFH, validating the bioinformatics findings. An ROC curve analysis confirmed the diagnostic potential of the hub genes. The top 10 hub genes show promise as ERS-related diagnostic biomarkers for SONFH. We discovered that 261 miRNAs, including hsa-miR-23, influence these genes and identified potential therapeutics such as dibenziodolium and simvastatin. Immune profiling indicated altered immune functions in SONFH, with significant correlations among immune cell types. Validation confirmed the upregulation of STAT3, IL1B, TLR2 and CASP1, which had diagnostic potential. The findings suggest potential diagnostic markers and therapeutic targets for SONFH.

Cellulose plays an important role in maintaining or improving soil carbon (C) cycling and soil fertility of paddy field. There had close relationship between functional cellulose genes ( cbhI and GH48 ) with characterize of soil organic matter chemical components (fulvic acid and humic acid) and soil physical fractions. However, there is still limited information about how functional cellulose degradation response to long-term fertilizer management and their relative importance for C sequestration under the double-cropping rice paddy field in southern of China. Therefore, the objective of this study were investigated the effects of 34-years long-term fertilizer regime on community abundance of cbhI and GH48 genes in five soil particle-size fractions (> 2000 μm, 2000–200 μm, 200–50 μm, 50–2 μm and 2–0.1 μm) by using polarization magic angle spinning 13 C nuclear magnetic resonance spectroscopy. The field experiment was included four different fertilizer treatments: chemical fertilizer alone (MF), rice straw and chemical fertilizer (RF), 30% organic manure and 70% chemical fertilizer (OM), and without fertilizer input as a control (CK). The results showed that distribution of soil humus and cellulolytic microbial community abundance was significant increased under long-term application of crop residue and organic manure condition. And the FA, HA and HM C contents in > 2000 μm and 2000–50 μm fractions with MF, RF and OM treatments were significant higher than that of CK treatment. Meanwhile, the alkyl C and Oalkyl C groups of FA and HA in > 2000 μm fraction with MF, RF, OM and CK treatments were higher than that of the other fractions. There had higher AL% and lower ARO% of FA and HA in different particle-size fractions with MF, RF, OM and CK treatments. The results indicated that abundance of cbhI and GH48 genes in different particle-size fractions with RF and OM treatments were significant increased, compared with CK treatment. There had significant positive correlation between soil humus C components (FA and HA) with abundance of cbhI and GH48 genes, and the o-alkyl C and AL% of FA were positively correlated with abundance of cbhI and GH48 genes. As a result, the community abundance of cbhI and GH48 genes were significant increased under combined application of crop residue and organic manure with chemical fertilizer condition.

Soil microorganism plays an important role in nitrogen (N) fixation process of paddy field, but the related information about how soil microorganism that drive N fixation process response to change of soil phy-chemical characteristics under the double-cropping rice ( Oryza sativa L.) paddy field in southern of China is need to further study. Therefore, the impacts of 34-years different long-term fertilization system on soil N-fixing bacteria community under the double-cropping rice paddy field in southern of China were investigated by taken chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) method in this paper. The field experiment were set up four different fertilizer treatments: chemical fertilizer alone (MF), rice straw and chemical fertilizer (RF), 30% organic manure and 70% chemical fertilizer (OM), and unfertilized as a control (CK). This results showed that compared with CK treatment, the diversity index of cbbL R and nifH genes with OM and RF treatments were significantly increased ( p <0.05), respectively. Meanwhile, the abundance of cbbL R gene with OM, RF and MF treatments were increased by 23.94, 12.19 and 6.70×10 7 copies g -1 compared to CK treatment, respectively. Compared with CK treatment, the abundance of nifH gene with OM, RF and MF treatments were increased by 23.90, 8.82 and 5.40×10 9 copies g -1 , respectively. This results indicated that compared with CK treatment, the soil autotrophic azotobacter and nitrogenase activities with OM and RF treatments were also significantly increased ( p <0.05), respectively. There were an obvious difference in features of soil N-fixing bacteria community between application of inorganic fertilizer and organic manure treatments. Therefore, this results demonstrated that abundance of soil N-fixing bacteria community in the double-cropping rice paddy field were increased by long-term applied with organic manure and crop residue managements.

Background Osteonecrosis of the femoral head (ONFH) significantly impacts young and middle-aged adults, with steroid use implicated in many cases. Traditional treatments have limited efficacy, prompting a shift towards innovative approaches, such as stem cell therapy, offering less invasive regenerative solutions. Methods Using bibliometric analysis from 1997 to 2023, we identified 392 articles on stem cell therapy for ONFH from the Web of Science Core Collection and analysed them using VOSviewer and CiteSpace to identify key trends and research directions. Results From 1997 to 2023, stem cell therapy for ONFH research expanded significantly, with 392 articles evidencing global collaboration, particularly from China, the United States and South Korea. The field is characterised by 158 core authors across 26 clusters and contributions from 417 institutions in 104 research clusters, with Shanghai Jiao Tong University as a notable leader. This research is disseminated through 23 journal clusters, emphasising interdisciplinary work, with Clinical Orthopaedics and Related Research among the most influential journals. Key findings include the identification of the most influential papers, highlighting advances, such as use of autologous mesenchymal stem cells (MSCs) and innovative delivery mechanisms. High-frequency keyword analysis further mapped the evolution of the field, from basic mechanisms to advanced therapies, underscoring a trend towards more targeted stem cell treatments for ONFH. Conclusion Stem cell therapy for ONFH has advanced significantly, showcasing a successful transition from basic research to clinical practice, particularly highlighted by developments in use of autologous MSCs and delivery methods. Future research will focus on refining therapies through exosome technology, targeted modulation of stress and inflammation and integration with surgical techniques, with the aim of tailored patient care and improved ONFH outcomes.

Soil microbial community were usually reconsidered as a sensitive indicator in soil quality and soil environment change of paddy field. However, the effects of different tillage and crop residue incorporation managements on soil bacterial community under the double-cropping rice cropping system were still need to further investigated. Therefore, the impacts of different tillage and crop residue incorporation managements on soil bacterial community under the double-cropping rice cropping system in southern of China were studied by using phospholipid fatty acids (PLFAs) profile method in the present paper. The experiment included four different tillage treatments: rotary tillage without crop residue input as a control (RTO), no-tillage with crop residue retention (NT), rotary tillage with crop residue incorporation (RT), and conventional tillage with crop residue incorporation (CT). Compared with RTO treatment, grain yield of rice with NT, RT and CT treatments increased by 1.21%, 3.13% and 6.40%, respectively. This results showed that soil aC15:0, C16:0, iC17:0, C19:0c9, 10 fatty acids with CT and RT treatments were higher than that of RTO treatment, while soil C16:1ω6c and C18:1ω9t fatty acids with NT treatment were higher than that of RTO treatment, respectively. Soil G + and G − bacteria PLFAs contents with CT treatment were higher than that of NT, RT and RTO treatments, while the value of soil G + /G − bacteria PLFAs with NT treatment were higher than that of CT, RT and RTO treatments. This results indicated that Richness and McIntosh indices with CT treatment were significantly higher than that of RTO treatment. Principal component analysis (PCA) results showed that the first and second principal components (PC1 and PC2) were explained 93.2% of total variance with all tillage treatments. Except C12:0, C14:0 2OH and C18:2ω6, all unsaturated and cyclopropyl PLFAs contents were belong to PC1. PC1 and PC2 were explained 88.4% of total variance with all tillage treatments. There had significantly positive correlation between soil Richness, Shannon indices and soil PLFAs, G + bacteria, G − bacteria, fungi contents. As a result, it were benefit practices for increasing soil bacterial community structure in the double-cropping rice field of southern China by combined application of rotary, conventional tillage with crop residue incorporation managements.