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Microalgae of different evolutionary origins are typically found in rivers, lakes, and oceans, providing more than 45% of global primary production. They provide not only a food source for animals, but also affect microbial ecosystems through symbioses with microorganisms or secretion of some metabolites. Derived from amino acids, polyamines are present in almost all types of organisms, where they play important roles in maintaining physiological functions or against stress. Microalgae can produce a variety of distinct polyamines, and the polyamine content is important to meet the physiological needs of microalgae and may also affect other species in the environment. In addition, some polyamines produced by microalgae have medical or nanotechnological applications. Previous studies on several types of microalgae have indicated that the putative polyamine metabolic pathways may be as complicated as the genomes of these organisms, which contain genes originating from plants, animals, and even bacteria. There are also several novel polyamine synthetic routes in microalgae. Understanding the nature of polyamines in microalgae will not only improve our knowledge of microalgal physiology and ecological function, but also provide valuable information for biotechnological applications.

White spot syndrome virus (WSSV) is the causative agent of white spot syndrome (WSS), a disease that has led to severe mortality rates in cultured shrimp all over the world. The WSSV is a large, ellipsoid, enveloped double-stranded DNA virus with a wide host range among crustaceans. Currently, the main antiviral method is to block the receptor of the host cell membrane using recombinant viral proteins or virus antiserum. In addition to interference with the ligand-receptor binding, disrupting the structure of the virus envelope may also be a means to combat the viral infection. Carbon quantum dots (CQDs) are carbonaceous nanoparticles that have many advantageous characteristics, including small size, low cytotoxicity, cheap, and ease of production and modification. Polyamine-modified CQDs (polyamine CQDs) with strong antibacterial ability have been identified, previously. In this study, polyamine CQDs are shown to attach to the WSSV envelope and inhibit the virus infection, with a dose-dependent effect. The results also show that polyamine CQDs can upregulate several immune genes in shrimp and reduce the mortality upon WSSV infection. This is first study to identify that polyamine CQDs could against the virus. These results, indeed, provide a direction to develop effective antiviral strategies or therapeutic methods using polyamine CQDs in aquaculture.

This paper investigates a piezoelectric energy harvester that consists of a piezoelectric cantilever and a tip mass for horizontal rotational motion. Rotational motion results in centrifugal force, which causes the axial load on the beam and alters the resonant frequency of the system. The piezoelectric energy harvester is installed on a rotational hub in three orientations—inward, outward, and tilted configurations—to examine their influence on the performance of the harvester. The theoretical model of the piezoelectric energy harvester is developed to explain the dynamics of the system and experiments are conducted to validate the model. Theoretical and experimental studies are presented with various tilt angles and distances between the harvester and the rotating center. The results show that the installation distance and the tilt angle can be used to adjust the resonant frequency of the system to match the excitation frequency.

Carbon quantum dots (CQDs) are emerging novel nanomaterials with a wide range of applications and high biocompatibility. However, there is a lack of in-depth research on whether CQDs can cause acute or long-term adverse reactions in aquatic organisms. In this study, two different types of CQDs prepared by ammonia citrate and spermidine, namely CQDAC and CQDSpd, were used to evaluate their biocompatibilities. In the fish embryo acute toxicity test (FET), the LD50 of CQDAC and CQDSpd was about 500 and 100 ppm. During the stage of eleutheroembryo, the LD50 decreased to 340 and 55 ppm, respectively. However, both CQDs were quickly eliminated from embryo and eleutheroembryo, indicating a lack of bioaccumulation. Long-term accumulation of CQDs was also performed in this study, and adult zebrafish showed no adverse effects in 12 weeks. In addition, there was no difference in the hatchability and deformity rates of offspring produced by adult zebrafish, regardless of whether they were fed CQDs or not. The results showed that both CQDAC and CQDSpd have low toxicity and bioaccumulation to zebrafish. Moreover, the toxicity assay developed in this study provides a comprehensive platform to assess the impacts of CQDs on aquatic organisms in the future.

Background Most tail-anchored (TA) membrane proteins are delivered to the endoplasmic reticulum through a conserved posttranslational pathway. Although core mechanisms underlying the targeting and insertion of TA proteins are well established in eukaryotes, their role in mediating TA protein biogenesis in plants remains unclear. We reported the crystal structures of algal arsenite transporter 1 (ArsA1), which possesses an approximately 80-kDa monomeric architecture and carries chloroplast-localized TA proteins. However, the mechanistic basis of ArsA2, a Get3 (guided entry of TA proteins 3) homolog in plants, for TA recognition remains unknown. Results Here, for the first time, we present the crystal structures of the diatom Pt-Get3a that forms a distinct ellipsoid-shaped tetramer in the open (nucleotide-bound) state through crystal packing. Pulldown assay results revealed that only tetrameric Pt-Get3a can bind to TA proteins. The lack of the conserved zinc-coordination CXXC motif in Pt-Get3a potentially leads to the spontaneous formation of a distinct parallelogram-shaped dimeric conformation in solution, suggesting a new dimer state for subsequent tetramerization upon TA targeting. Pt-Get3a nonspecifically binds to different subsets of TA substrates due to the lower hydrophobicity of its α-helical subdomain, which is implicated in TA recognition. Conclusions Our study provides new insights into the mechanisms underlying TA protein shielding by tetrameric Get3 during targeting to the diatom’s cell membrane.

Background Shrimp aquaculture has suffered huge economic losses over the past decade due to the outbreak of acute hepatopancreatic necrosis disease (AHPND), which is mainly caused by the bacteria Vibrio parahaemolyticus ( V. parahaemolyticus ) with the virulence pVA1 plasmid, which encodes a secretory photorhabdus insect-related (Pir) toxin composed of PirA and PirB proteins. The Pir toxin mainly attacks the hepatopancreas, a major metabolic organ in shrimp, thereby causing necrosis and loss of function. The pandemic of antibiotic-resistant strains makes the impact worse. Methods Mild pyrolysis of a mixture of polysaccharide dextran 70 and the crosslinker 1,8-diaminooctane at 180 ℃ for 3 h to form carbonized nanogels (DAO/DEX-CNGs) through controlled cross-linking and carbonization. The multifunctional therapeutic CNGs inherit nanogel-like structures and functional groups from their precursor molecules. Results DAO/DEX-CNGs manifest broad-spectrum antibacterial activity against Vibrio parahaemolyticus responsible for AHPND and even multiple drug-resistant strains. The polymer-like structures and functional groups on graphitic-carbon within the CNGs exhibit multiple treatment effects, including disruption of bacterial membranes, elevating bacterial oxidative stress, and neutralization of PirAB toxins. The inhibition of Vibrio in the midgut of infected shrimp, protection of hepatopancreas tissue from Pir toxin, and suppressing overstimulation of the immune system in severe V. parahaemolyticus infection, revealing that CNGs can effectively guard shrimp from Vibrio invasion. Moreover, shrimps fed with DAO/DEX-CNGs were carefully examined, such as the expression of the immune-related genes, hepatopancreas biopsy, and intestinal microbiota. Few adverse effects on shrimps were observed. Conclusion Our work proposes brand-new applications of multifunctional carbon-based nanomaterials as efficient anti- Vibrio agents in the aquatic industry that hold great potential as feed additives to reduce antibiotic overuse in aquaculture. Graphical Abstract

Aurantiochytrium limacinum holds great promise for producing sustainable single-cell oil as an alternative to fish oil. However, research into its complex biological and biochemical characteristics and efforts toward strain improvement have been hampered by insufficient genetic tools. Until now, genetic transformations of A. limacinum have relied solely on chromosome integration, which is inefficient and prone to insertional mutagenesis and other issues related to genetically modified organisms (GMOs). This paper describes the first centromeric plasmid for A. limacinum . Amplification of this shuttle vector by E. coli enables direct delivery into A. limacinum via electroporation, where it undergoes stable replication and segregation into daughter cells. The key to the stable plasmid maintenance lies in a 500 bp segment derived from chromosome 24 of Phaeodactylum tricornutum . While this segment does not significantly enhance the efficiency of vector transformation, it enables the replication and maintenance of the shuttle vector in the host cell as closed circular DNA. The plasmid from three transformants demonstrates a high segregation efficiency of 96.8 ± 0.3% ( n  = 3), even in the absence of antibiotic selection. This novel centromeric plasmid considerably enhances the flexibility of genetic manipulations and gene expression in A. limacinum , opening new avenues for its study and industrial application. Key points • First centromeric plasmid developed for genetic transformation in A. limacinum. • The novel plasmid enhances flexibility in genetic manipulation and gene expression. • The plasmid achieves 96.8 ± 0.3% (n = 3) segregation efficiency without antibiotic selection.

In this study, 101 and 102 rainwater samples were collected from April 2020 to February 2024 in a coastal and marine area, respectively. The results show that the Cl/Na ratios in both study areas were lower than the seawater value (1.17), suggesting chloride depletion. The chloride depletion rates in both areas decreased after the COVID-19 lockdown period. The molar ratio of NH4+ to non-sea-salt sulfate (nss-SO42−) was 1.54, with a mixture of (NH4)2SO4 and NH4HSO4 in the coastal area, and 0.83, with NH4HSO4 as the main form, in the marine area. A decreasing trend attributed to high O3 and relative humidity (RH) levels occurred in 2022. Among the dissolved organic nitrogen (DON) species, DON accounted for 24% and 32% of the total dissolved nitrogen (TDN) in the coastal and marine areas, respectively, indicating a greater relative contribution under lower anthropogenic influence. On the basis of the correlation between the species and source analysis results, NO3− mainly originated from fossil fuel combustion, NH4+ originated from agricultural emissions and secondary aerosols, and DON originated from secondary aerosols via combustion processes and natural emissions. In terms of the flux, due to lockdown activities, the Dissolved Inorganic Nitrogen (DIN) flux decreased significantly from 40.6 to 19.3 mmol m−2 yr−1 in the coastal area and from 27.7 to 15.1 mmol m−2 yr−1 in the marine area. Additionally, a slight decrease occurred in the DON flux, from 21.6 to 19.3 mmol m−2 yr−1 and from 27.7 to 15.1 mmol m−2 yr−1, respectively. Regarding new production, based on nitrogen input, the level in the coastal area decreased from 5.83 to 2.10 g C m−2 yr−1, and that in the marine area decreased from 3.92 to 1.55 g C m−2 yr−1, indicating a significant reduction during the COVID-19 lockdown.

Food safety concerns regarding foodborne pathogen contamination have gained global attention due to its significant implications. In this study, we developed a detection system utilizing a PCR array combined with an automated magnetic bead-based system and CE technology to enable the detection of three foodborne pathogens, namely Salmonella enterica, Listeria monocytogenes, and Staphylococcus aureus. The results showed that our developed method could detect these pathogens at concentrations as low as 7.3 × 101, 6.7 × 102, and 6.9 × 102 cfu/mL, respectively, in the broth samples. In chicken samples, the limit of detection for these pathogens was 3.1 × 104, 3.5 × 103, and 3.9 × 102 cfu/g, respectively. The detection of these pathogens was accomplished without the necessity for sample enrichment, and the entire protocols, from sample preparation to amplicon analysis, were completed in approximately 3.5 h. Regarding the impact of the extraction method on detection capability, our study observed that an automated DNA extraction system based on the magnetic bead method demonstrated a 10-fold improvement or, at the very least, yielded similar results compared to the column-based method. These findings demonstrated that our developed model is effective in detecting low levels of these pathogens in the samples analyzed in this study. The PCR-CE method developed in this study may help monitor food safety in the future. It may also be extended to identify other foodborne pathogens across a wide range of food samples.

The study of the sinking phenomenon of diatom cells, which have a slightly larger specific gravity (~1.3) compared to that of water, is an important research topic for understanding photosynthetic efficiency. In this study, we successfully demonstrated the observation of the sinking behaviors of four different species of diatom using a homemade “tumbled” optical microscope. A homemade 1 mm 3 microchamber was employed to decrease the effects of convection currents. In the microchamber, diatom cells were basically settled in a linear manner without floating, although some of the cells were rotated during their sinking. Sinking speeds of the four species of diatom cells, Nitzschia sp., Pheodactylum tricornutum , Navicula sp., and Odontella aurita , were 0.81 ± 5.56, 3.03 ± 10.17, 3.29 ± 7.39, and 11.22 ± 21.42 μ m/s, respectively, based on the automatic tracking analysis of the centroids of each cell. Manual analysis of a vector between two longitudinal ends of the cells (two-point analysis) was effective for quantitatively characterizing the rotation phenomenon; therefore, angles and angular velocities of rotating cells were well determined as a function of time. The effects of the cell shapes on sinking velocity could be explained by simulation analysis using the modified Stokes’ law proposed by Miklasz et al.