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Ag@C fibres with porous silver structure were synthesized with a surface-hydrolyzed polyacrylonitrile (PAN) fibre as a template and by a hydrazine hydrate reduction and carbonization process. The composition and structure of the Ag@C fibres were characterized with a scanning electron microscope, an X-ray diffractometer, a Fourier transform infrared spectrometer, an X-ray photoelectron spectrometer, a Raman spectrometer, and a thermogravimetric analyser. The porous structure of the silver shell combined with carbon was conducive to the release of silver ions and suppressed the loss of silver nanoparticles from the surface of the carbon fibres. The Ag@C fibres showed excellent antibacterial performance. When the dosage of Ag@C fibres prepared with a silver nitrate solution of 0.5 mol/L was 1 mg, 2 mg, 4 mg and 8 mg, the bacterial inactivation rate to Staphylococcus aureus was 97.98%, 99.89%, 99.99%, and 99.99% in 12 h, respectively. The loss of viability only decreased to 80% when 1 mg of Ag@C fibres were used for antimicrobial activity test after five times of recycling. The Ag@C fibres have a broad application prospect in producing fabric products of antibacterial fields.

The effects of Enterococcus faecium on growth, intestinal barrier function, and immune response in Escherichia coli O78-challenged broiler chickens were investigated. Three hundred eight 1-day-old Ross male chickens were randomly assigned into three treatment groups: negative control (C), E. coli O78-infected positive (EP), and E. coli O78-infected with 200 mg/kg E. faecium dietary supplementation (EF). E. faecium significantly increased the body weight on day 10 ( P  < 0.05) and day 15. Furthermore, these birds had a greater average daily gain compared with the other groups during days 1–10 ( P  < 0.05). The death rate of the EF chickens dramatically declined. E. faecium supplementation improved the jejunal villus height and the ratio of villus height to crypt depth ( P  < 0.05) 3 and 7 days post-infection. The mRNA expression of claudin-1 significantly increased by E. faecium ( P  < 0.05) 3 and 7 days post-infection, and Mucin2 was markedly enhanced ( P  < 0.05) 3 days post-infection. E. faecium upregulated the mRNA expression of PPAR-γ and IL-10 ( P  < 0.05) and downregulated that of NF-κB , TLR4 , and IL-1β ( P  < 0.05) in the spleen 3 and 7 days post-infection. Lipopolysaccharide stimulation index was markedly enhanced in the EF group ( P  < 0.05) 3 days post-infection. The increased liver E. coli number caused by the E. coli O78 challenge was significantly reversed by E. faecium ( P  < 0.05). E. faecium improved growth and reduced the death rate by regulating the immune response and maintaining the intestinal integrity in E. coli O78-challenged broiler chickens.

The rapid growth of China’s prepared food industry has created new opportunities for industrial upgrading, but it has also intensified concerns regarding product quality, supervision gaps, and the long-term sustainability of governance structures. In response to these challenges, this study develops a tripartite evolutionary game model involving local governments, enterprises, and consumers, and further integrates a Markov chain framework to capture stochastic disturbances and long-run state transitions. This dynamic–stochastic modeling approach enables an examination of how collaborative supervision evolves under varying regulatory intensities, compliance costs, and consumer reporting costs. The results show that (1) multi-actor collaborative supervision substantially increases firms’ incentives to operate honestly and reinforces positive feedback loops between regulators and consumers; (2) excessive regulatory, compliance, or reporting costs weaken system stability and reduce policy effectiveness; (3) aligning regulatory intensity with penalty mechanisms accelerates the system’s convergence toward a stable equilibrium, balancing industrial development with food safety objectives; and (4) Markov chain simulations confirm the robustness and long-term stationarity of the governance system. Overall, this study provides a dynamic and evidence-based framework for designing sustainable and resilient regulatory mechanisms in the prepared food industry. The findings offer practical guidance for advancing Sustainable Development Goals (SDGs) 3, 12, and 16 through improved food safety, responsible production, and stronger institutional governance.

Epidermal growth factor receptor tyrosine kinase inhibitors (EGFR-TKIs) are effective against non-small cell lung cancer (NSCLC) with EGFR -activating mutations. The mechanisms underlying EGFR-TKI resistance are not fully understood. This study aimed to analyze the effects of seven EGFR ligands on EGFR-TKI sensitivity in NSCLC cells and patients. Cells with EGFR E746-A750del mutation were treated with recombinant EGFR ligands, and analyzed for cell viability, proliferation, and apoptosis. shRNA knockdown of endogenous Epiregulin (EREG) or overexpression of exogenous EREG and immunofluorescence experiments were carried out. Public gene expression datasets were used for tumor microenvironment and clinical assessment. Among the EGFR ligands, EREG significantly diminished cellular sensitivity to TKIs and was associated with decreased response to erlotinib in NSCLC patients. EREG induced AKT phosphorylation and attenuated TKI-induced cellular apoptosis in an ErbB2-dependent manner. EREG induced the formation of the EGFR/ErbB2 heterodimer regardless of gefitinib treatment. However, overexpression or knockdown of EREG in cancer cells had little impact on TKI sensitivity. Single-cell RNA sequencing data revealed that EREG was predominantly expressed in macrophages in the tumor microenvironment. In addition, EREG-enriched macrophage conditional medium induced EGFR-TKI resistance. These findings shed new light on the mechanism underlying EGFR-TKI resistance, and suggest macrophage-produced intratumoral EREG as a novel regulator and biomarker for EGFR-TKI therapy in NSCLC.

Multiple studies have identified conserved genetic pathways and small molecules associated with extension of lifespan in diverse organisms. However, extending lifespan does not result in concomitant extension in healthspan, defined as the proportion of time that an animal remains healthy and free of age-related infirmities. Rather, mutations that extend lifespan often reduce healthspan and increase frailty. The question arises as to whether factors or mechanisms exist that uncouple these processes and extend healthspan and reduce frailty independent of lifespan. We show that indoles from commensal microbiota extend healthspan of diverse organisms, including Caenorhabditis elegans, Drosophila melanogaster, and mice, but have a negligible effect on maximal lifespan. Effects of indoles on healthspan in worms and flies depend upon the aryl hydrocarbon receptor (AHR), a conserved detector of xenobiotic small molecules. In C. elegans, indole induces a gene expression profile in aged animals reminiscent of that seen in the young, but which is distinct from that associated with normal aging. Moreover, in older animals, indole induces genes associated with oogenesis and, accordingly, extends fecundity and reproductive span. Together, these data suggest that small molecules related to indole and derived from commensal microbiota act in diverse phyla via conserved molecular pathways to promote healthy aging. These data raise the possibility of developing therapeutics based on microbiota-derived indole or its derivatives to extend healthspan and reduce frailty in humans.

Background CD3 + CD20 + T cells (T B cells) are a subset of lymphocytes in the human body that are associated with inflammation. They originate from T cells interacting with B cells, and their levels are abnormally elevated in individuals with immune disorders, as well as in some cancer patients. The interplay between tumor immunity and inflammation is intricate, yet the specific involvement of T B cells in local tumor immunity remains uncertain, with limited research on their subtypes. Methods Lung cancer surgical samples were stained using multi-color immunofluorescence to study the subtypes and distribution patterns of T B cells. Results T B cells were confirmed to exist in a scattered pattern within tertiary lymphoid structures (TLS) in lung cancer tissues, with higher abundance in mature TLS. In subtype analysis, the CD4-CD8- double-negative T B cell subtype was predominant, comprising over 90% in samples with abundant TLS infiltration and over 60% in samples with poor infiltration. This was followed by the CD4 + CD8- and CD4-CD8 + single-positive T B cell subtypes, while the CD4 + CD8 + double-positive T B cell subtype was nearly absent. During the maturation of TLS, the proportion of B cells gradually increased, while the proportion of CD4-CD8- T cell subtype decreased. Conclusions T B cells extensively infiltrate the TLS regions in tumor tissues, with the double-negative subtype being predominant, potentially playing a crucial regulatory role in the local tumor immune microenvironment. This finding could facilitate the advancement of novel cancer treatment strategies.

The resident prokaryotic microbiota of the metazoan gut elicits profound effects on the growth and development of the intestine. However, the molecular mechanisms of symbiotic prokaryotic–eukaryotic cross‐talk in the gut are largely unknown. It is increasingly recognized that physiologically generated reactive oxygen species (ROS) function as signalling secondary messengers that influence cellular proliferation and differentiation in a variety of biological systems. Here, we report that commensal bacteria, particularly members of the genus Lactobacillus , can stimulate NADPH oxidase 1 (Nox1)‐dependent ROS generation and consequent cellular proliferation in intestinal stem cells upon initial ingestion into the murine or Drosophila intestine. Our data identify and highlight a highly conserved mechanism that symbiotic microorganisms utilize in eukaryotic growth and development. Additionally, the work suggests that specific redox‐mediated functions may be assigned to specific bacterial taxa and may contribute to the identification of microbes with probiotic potential. Non‐pathogenic gut bacteria promote ROS generation in host epithelial cells, thereby regulating intestinal homeostasis in both mammals and insects.

Based on the half-hourly Integrated Multi-Satellite Retrievals for Global Precipitation Measurement (IMERG) data product (0.1°), the fifth-generation European Center for Medium-Range Weather Forecasting atmospheric reanalysis dataset (ERA5), sounding data, and the Weather Research and Forecasting Model (WRF-ARW), this study explored the developmental process of a typical extreme precipitation event in Liaoning Province on 2 June 2021. This study focused on the impact of Changbai Mountain on this precipitation process and its corresponding physical mechanisms. The research findings revealed that Changbai Mountain significantly affected the precipitation event in three main aspects: blocking drag, forcing uplift, and leeside convergence. The blocking drag caused by the mountain topography led to an extension in the duration of heavy rainfall. The dynamic lifting and leeside convergence associated with the mountainous terrain also substantially increased the amount of precipitation. Furthermore, the topography hindered the movement of the Bohai Bay cold pool and enhanced the intensity of the cold pool, contributing to the sustained extreme precipitation in Liaoning Province. Lastly, the terrain sensitivity experiment demonstrated that when the height of Changbai Mountain was reduced, the convergence uplift, moisture condensation, and cold pool intensity were weakened, leading to significant changes in precipitation intensity and spatial distribution. These findings further confirm the crucial role of Changbai Mountain in the occurrence and development of local precipitation in Liaoning Province.

Cold-chain supply systems depend on a sequence of linked production and logistics decisions. In prepared-food cold chains, quality may deteriorate not because one visible failure occurs, but because testing, traceability records, temperature monitoring, and abnormal-condition reporting are gradually weakened under cost pressure. Once such hidden effort reduction accumulates, external disturbances may push the system from strict assurance to weakened governance. To explain this nonlinear process, an asymmetric evolutionary game is built between prepared-food producers and cold-chain logistics providers, each choosing between strict and weakened quality assurance. White Gaussian noise is introduced to represent random operating shocks, and the two-population strategy system is projected onto a system-level quality-governance coordinate, q. This projection is used as a transparent baseline coordinate rather than as an assumption of linear system evolution. The reduced system is then transformed into a stochastic cusp catastrophe model, with a resilience indicator used to measure the distance from critical transition conditions. Numerical simulations show that quality assurance costs and short-term cost-saving benefits move the system toward a weakened-governance basin, whereas external incentives, coordination degree, and credible accountability mechanisms support recovery toward strict collaboration. The framework offers a scenario-based resilience diagnosis approach for identifying threshold effects in cold-chain quality governance. Digital traceability, temperature-data sharing, incentive alignment, and accountability rules are further interpreted as operational innovations that improve resilience and reduce avoidable quality losses in sustainable cold-chain operations.

Rabies is a lethal zoonotic disease that is mainly caused by the rabies virus (RABV). Although effective vaccines have long existed, current vaccines take both time and cost to produce. Messenger RNA (mRNA) technology is an emergent vaccine platform that supports rapid vaccine development on a large scale. Here, an optimized mRNA vaccine construct (LVRNA001) expressing rabies virus glycoprotein (RABV-G) was developed in vitro and then evaluated in vivo for its immunogenicity and protective capacity in mice and dogs. LVRNA001 induced neutralizing antibody production and a strong Th1 cellular immune response in mice. In both mice and dogs, LVRNA001 provided protection against challenge with 50-fold lethal dose 50 (LD 50 ) of RABV. With regards to protective efficiency, an extended dosing interval (14 days) induced greater antibody production than 3- or 7-day intervals in mice. Finally, post-exposure immunization against RABV was performed to evaluate the survival rates of dogs receiving two 25 μg doses of LVRNA001 vs. five doses of inactivated vaccine over the course of three months. Survival rate in the LVRNA001 group was 100%, whereas survival rate in the inactivated vaccine control group was only 33.33%. In conclusion, these results demonstrated that LVRNA001 induced strong protective immune responses in mice and dogs, which provides a new and promising prophylactic strategy for rabies.