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The moon day diet : kick-start conscious habits in the kitchen (and in life) with over 150 plant-based recipies and the principles of yoga and mindfulness
\"In The Moon Day Diet, author, behavior specialist, and co-founder of one of Philadelphia PA's largest yoga communities Dana Hot Yoga, Dorian Mitchell shares the insights that encouraged him to drastically reduce meat and dairy consumption in a way that is sustainable for a lifetime versus cold turkey methods. This revolutionary new guide translates the basic principles of yoga into the realm of diet. He outlines the incredible benefits of reducing your consumption of animal products and increasing your intake of plant-based foods, including effortless weight loss, better health, and more mindfulness and compassion in your thoughts and decisions each day.\"--Publisher's description.
Book
Chromothripsis as an on-target consequence of CRISPR–Cas9 genome editing
Genome editing has therapeutic potential for treating genetic diseases and cancer. However, the currently most practicable approaches rely on the generation of DNA double-strand breaks (DSBs), which can give rise to a poorly characterized spectrum of chromosome structural abnormalities. Here, using model cells and single-cell whole-genome sequencing, as well as by editing at a clinically relevant locus in clinically relevant cells, we show that CRISPR–Cas9 editing generates structural defects of the nucleus, micronuclei and chromosome bridges, which initiate a mutational process called chromothripsis. Chromothripsis is extensive chromosome rearrangement restricted to one or a few chromosomes that can cause human congenital disease and cancer. These results demonstrate that chromothripsis is a previously unappreciated on-target consequence of CRISPR–Cas9-generated DSBs. As genome editing is implemented in the clinic, the potential for extensive chromosomal rearrangements should be considered and monitored.
Chromothripsis, a chromosomal shattering event, can be elicited by micronuclei and chromosome bridges formed by CRISPR–Cas9-generated double-stranded breaks. Extensive chromosomal rearrangements may thus be an on-target effect of genome editing.
Journal Article
Working stiff : two years, 262 bodies, and the making of a medical examiner
\"The fearless memoir of a young forensic pathologist's \"rookie season\" as a NYC medical examiner, and the cases--hair-raising and heartbreaking and impossibly complex--that shaped her as both a physician and a mother. Just two months before the September 11 terrorist attacks, Dr. Judy Melinek began her training as a New York City forensic pathologist. With her husband T.J. and their toddler Daniel holding down the home front, Judy threw herself into the fascinating world of death investigation--performing autopsies, investigating death scenes, counseling grieving relatives. Working Stiff chronicles Judy's two years of training, taking readers behind the police tape of some of the most harrowing deaths in the Big Apple, including a firsthand account of the events of September 11, the subsequent anthrax bio-terrorism attack, and the disastrous crash of American Airlines flight 587. Lively, action-packed, and loaded with mordant wit, Working Stiff offers a firsthand account of daily life in one of America's most arduous professions, and the unexpected challenges of shuttling between the domains of the living and the dead. The body never lies--and through the murders, accidents, and suicides that land on her table, Dr. Melinek lays bare the truth behind the glamorized depictions of autopsy work on shows like CSI and Law & Order to reveal the secret story of the real morgue\"-- Provided by publisher.
Book
Arsenic in drinking water: An analysis of global drinking water regulations and recommendations for updates to protect public health
Evidence-based public health policy often comes years or decades after the underlying scientific breakthrough. The World Health Organization’s (WHO’s) provisional 10 μg/L arsenic (As) drinking water guideline was set in 1993 based on “analytical achievability.” In 2011, an additional proviso of “treatment performance” was added; a health-based risk assessment would lead to a lower and more protective guideline. Since the WHO does not require United Nations member states to submit copies of national drinking water regulations, there is no complete database of national drinking water standards or guidelines. In this study, we collated and analyzed all drinking water regulations for As from national governments worldwide. We found regulations for 176 countries. Of these countries, 136 have drinking water regulations that specify 10 μg/L As or less, while 40 have regulations that allow more than 10 μg/L of As; we could not find any evidence of regulations for 19 countries. The number of people living in countries that do not meet the WHO’s guideline constitutes 32% of the global population. Global As regulations are also strongly tied to national income, with high income countries more likely to meet the WHO’s guideline. In this study, we examined the health risk assessments that show a clear need for reducing As exposure to levels far below the current WHO provisional guideline. We also show that advances in analytical chemistry, drinking water treatment, and the possibility of accessing alternative drinking water supplies without As suggest that both low-income countries with limited resources and high-income countries with adequate resources can adopt a lower and more protective national drinking water standards or guidelines for As. Thus, we recommend that regulators and stake holders of all nations reassess the possibilities for improving public health and reducing health care expenses by adopting more stringent regulations for As in drinking water.
Journal Article
Isolation, establishment, and characterization of immortalized ileum bovine intestinal epithelial cell line
Primary intestinal epithelial cells are a powerful tool to study tight junction protein function and barrier integrity of epithelial cells. However, primary cells are costly, difficult to isolate, have a finite ability to replicate, and require the usage of live tissue. An alternative to primary cells is immortalized cell lines, which are cost effective, easily cultured, stored, and maintain the physiology of primary cells. Several immortalized lines are commercially available, yet there remains a need for cell lines for livestock research, specifically bovine. Here we report an immortalized bovine intestinal epithelial cell (IBEC) line was established by transfection with exogenous human telomerase reverse transcriptase (
hTERT
). Primary bovine intestinal epithelial cells (PBECs) were isolated from a 2-day old mixed breed calf. PBECs were transfected with
hTERT
to create an IBEC line. The transfected cells were positive for intestinal epithelial markers while testing negative for fibroblast markers. There was no significant difference in IBEC morphology or physiology post immortalization. In Trans-Epithelial Electrical Resistance (TEER assays), PBECs and IBECs behaved similarly to intestinal porcine enterocyte cells (IPEC-J2) under treatment of Iipopolysaccharide and calcium butyrate. This newly-developed cell line will be an essential and useful tool for in vitro bovine and ruminant intestinal research.
Journal Article
Biotechnology: Overcoming biological barriers to nucleic acid delivery using lipid nanoparticles
The promise of therapeutic nucleic acids has long been tempered by difficulty in overcoming biological barriers to their delivery. The past two decades have seen the development of ionizable lipid nanoparticles as a vehicle for nucleic acid delivery and their translation to the clinic.
Journal Article
Transformation of Adolescent Peer Relations in the Social Media Context: Part 1—A Theoretical Framework and Application to Dyadic Peer Relationships
Investigators have long recognized that adolescents’ peer experiences provide a crucial context for the acquisition of developmental competencies, as well as potential risks for a range of adjustment difficulties. However, recent years have seen an exponential increase in adolescents’ adoption of social media tools, fundamentally reshaping the landscape of adolescent peer interactions. Although research has begun to examine social media use among adolescents, researchers have lacked a unifying framework for understanding the impact of social media on adolescents’ peer experiences. This paper represents Part 1 of a two-part theoretical review, in which we offer a transformation framework to integrate interdisciplinary social media scholarship and guide future work on social media use and peer relations from a theory-driven perspective. We draw on prior conceptualizations of social media as a distinct interpersonal context and apply this understanding to adolescents’ peer experiences, outlining features of social media with particular relevance to adolescent peer relations. We argue that social media transforms adolescent peer relationships in five key ways: by changing the frequency or immediacy of experiences, amplifying experiences and demands, altering the qualitative nature of interactions, facilitating new opportunities for compensatory behaviors, and creating entirely novel behaviors. We offer an illustration of the transformation framework applied to adolescents’ dyadic friendship processes (i.e., experiences typically occurring between two individuals), reviewing existing evidence and offering theoretical implications. Overall, the transformation framework represents a departure from the prevailing approaches of prior peer relations work and a new model for understanding peer relations in the social media context.
Journal Article