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Paratuberculosis (PTB) is a chronic granulomatous enteritis caused by Mycobacterium avium subsp. paratuberculosis (Map). Vaccination is one of the most cost-effective tools for PTB control, although alternative treatments like the probiotic Dietzia have been explored with promising results. Using a rabbit model, we investigated the association of immunological and microbiota profiles in Gut Associated Lymphoid Tissue (GALT) with the effects in protection induced by the administration of Dietzia spp., the commercial vaccine (Silirum ® ) and the combination of both. The treatment with the probiotic diminished inflammation, but failed to control Map burden, suggesting a detrimental effect. Rabbits treated with the probiotic presented the highest rates of tissue lesion extension, although the immunological profile was not suggestive of an inflammatory state. Map load in both vaccinated groups was similar indicating that both treatments are equally effective in eliminating the infection, suggesting the role of vaccination in eliminating the infection prevails over the immunomodulatory effects of the probiotic. There were slight variations in the presence of some taxonomic groups depending on the treatment, highlighting the complexity of microbial interactions and the need to optimise treatment combinations in the context of each disease and animal species.

Germline genetic alterations and their associated cancer predisposition syndromes (CPS) are an important cause of pediatric cancer. Early recognition is of great importance for targeted surveillance, early detection, and prompt (personalized) therapeutic interventions. This review provides an overview of non-central nervous system solid pediatric tumor types, in relation to their associated CPS, with an emphasis on their histology. It serves as a guide for (pediatric) pathologists to increase their awareness of histological clues that suggest a CPS and warrant referral to the clinical geneticist.

Porous electrodes based on polymethylmethacrylate and graphite foams (PMMA_G_F) have been developed and characterized. Such devices have been successfully used as voltammetric sensors to analyze catechol, hydroquinone, and their mixtures. The presence of pores induces important changes in the oxidation/reduction mechanism of catechol and hydroquinone with respect to the sensing properties observed in nonfoamed PMMA_graphite electrodes (PMMA_G). The electropolymerization processes of catechol or hydroquinone at the electrode surface observed using PMMA_G do not occur at the surface of the foamed PMM_G_F. In addition, the limits of detection observed in foamed electrodes are one order of magnitude lower than the observed in the nonfoamed electrodes. Moreover, foamed electrodes can be used to detect simultaneously both isomers and a remarkable increase in the electrocatalytic properties shown by the foamed samples, produces a decrease in the oxidation potential peak of catechol in presence of hydroquinone, from +0.7 V to +0.3 V. Peak currents increased linearly with concentration of catechol in presence of hydroquinone over the range of 0.37·10−3 M to 1.69·10−3 M with a limit of detection (LOD) of 0.27 mM. These effects demonstrate the advantages obtained by increasing the active surface by means of porous structures.

The aim of this study was to evaluate the effect of ibuprofen on gastric mucosa and enzymes involved in gastroprotection in healthy volunteers. Twenty-four Helicobacter pylori-negative subjects were randomized to treatment with ibuprofen or ibuprofen-arginate (each 600 mg/6 hr during 3 days). Endoscopies were performed 1 week before and after treatment. Biopsies were taken from the gastric antrum and corpus for determination of prostaglandin E2 (PGE2) by ELISA and cyclooxygenase (COX-1 and COX-2) and nitric oxide synthase (eNOS and iNOS) by western blot. All subjects had at least one gastric lesion except for two individuals taking ibuprofen-arginate. Ibuprofen-arginate caused a lower rate of clinical adverse reactions than ibuprofen. Subjects with gastric lesions or adverse reactions had lower PGE2 levels. COX-1, COX-2, eNOS, and iNOS were detectable in all subjects. The constitutive enzymes (COX-1 and eNOS) did not change after treatment. COX-2 was higher in corpus than antrum and it increased after ibuprofen treatment. iNOS tended to increase mildly in the corpus in subjects with adverse reactions or endoscopic lesions. There were no significant differences between ibuprofen and ibuprofen-arginate in PGE2, or enzymes.

This paper studies the effectiveness of a type of nonprice promotion often used in the European magazines industry to diminish the decline rate of periodical sales, in which a value pack is sold containing the magazine issue plus another product. Magazines are sold simultaneously with and without promotion at different prices, and promotions are serialized by fractioning the additional product across different issues of the magazine. Although promoting magazines contemporarily may cannibalize nonpromoted sales, this loss is compensated by a long-term increase in nonpromoted sales caused by product awareness and loyalty improvements. This strategy is increasingly used as an innovative form of product advertising.

Adida et al determined the expression of survivin in neuroblastoma and its relation to disease progression. The expression of the gene in neuroblastoma correlates to a more aggressive disease.

Six cases are reported of an osteoclast-rich tumor of the gastrointestinal tract that should be segregated from GIST. Five of the cases were located in the small bowel and one in the stomach. The age of the patients ranged from 13 to 37 years. The tumors behaved aggressively, with metastases to regional lymph nodes, liver, and other intra-abdominal sites. Microscopically, the tumor cells were medium-sized, predominantly oval, relatively monomorphic, diffusely immunoreactive for S-100-protein, and negative for CD117, CD34, HMB-45, and Mart-1. They were admixed with scattered osteoclast-like, multinucleated giant cells which were S-100-protein negative and KP1-positive. One case studied cytogenetically had the karyotype 46XX t(12;22)(q13;q12). The cases here reported are interpreted as examples of a distinctive type of gastrointestinal neoplasm which shares some features with clear cell sarcoma of soft parts (melanoma of soft parts), including in one case the chromosomal translocation that is characteristically associated with that entity.

We aimed to elucidate the influence on analgesic effect of genetic polymorphisms in enzymes responsible for biotransformation of tramadol and ibuprofen or other possible genes involved in their mechanism of action. The study population comprised 118 patients from a multicenter, randomized, double-blind, placebo-controlled, Phase III clinical trial that assessed the analgesic efficacy and tolerability of a single dose of ibuprofen (arginine)/tramadol 400/37.5 mg compared with ibuprofen arginine 400 mg alone, tramadol 50 mg alone, and placebo in patients with moderate to severe pain after dental surgery. We analyzed 32 polymorphisms in the cytochrome P450 (CYP) enzymes COMT, ABCB1, SLC22A1, OPRM1, and SLC22A1. We did not find any statistically significant difference among CYP2C9 phenotypes related to ibuprofen response, although CYP2C9 poor metabolizers had a longer effect (higher pain relief at 6 hours). Likewise, we did not find any statistically significant difference among PTGS2 genotypes, contradicting previously publications. There was not a clear effect of CYP2D6 phenotype on tramadol response, although CYP2D6 poor metabolizers had a slower analgesic effect. Concerning the transport of CYP2D6, we observed a better response in individuals carrying ABCB1 mutated alleles, which might correlate with higher tramadol plasma levels. Finally, we found a statistically significant better response in patients carrying the OPRM1 A118G G allele, which contradicts the previous reports. Measuring the active metabolite O-desmethyl-tramadol formation would be of great importance to better evaluate this association because O-desmethyl-tramadol has a higher μ-opioid receptor affinity compared with the parent drug. EudraCT.ema.europa.eu identifier: 2013-004637-33.

Objetivo: Se examinó el estado de la investigación en las Ciencias de la Información (CI) en Cuba, a partir de una incursión empírica en las tesis de maestría y doctorado defendidas entre los años 2008 y 2018.Diseño/Metodología/Enfoque. Se emplearon indicadores bibliométricos de producción y colaboración científica, combinados con técnicas de análisis de redes sociales.Resultados/Discusión. Los resultados de producción apuntaron al alto carácter aplicado de las investigaciones y a su diversidad temática. La solución de problemas informacionales en los sectores de la educación superior fue el elemento más explorado en las tesis de doctorado. Mientras que las tesis de maestría se concentraron en los sectores empresarial, salud pública, así como otras instituciones de información. Con respecto a las relaciones de colaboración establecidas para la ejecución de las investigaciones, se detectaron diferencias sustanciales; dado que en las tesis de maestría son incipientes, mientras que en las de doctorado hubo fuertes relaciones con la Universidad de Granada, España.Conclusiones. El estudio de las tesis de posgrado de CI defendidas en Cuba confirma que es un campo profesional típicamente femenino, concentrado temáticamente en la alfabetización informacional, la evaluación de bibliotecas universitarias, la gestión documental, de información y del conocimiento en las organizaciones, y las investigaciones métricas. Los patrones de colaboración reflejan que la tutoría requiere ser perfeccionado.Originalidad/Valor. Este estudio ofrece información sistematizada, actualizada y relevante para la proyección estratégica de la formación cubana de postgrado en CI.

Fatty-acid oxidation has a major role in energy production during periods of fasting. When body glucose is depleted, fatty acids are mobilized from adipose tissue, taken up by the liver, and converted to ketone bodies, a major alternative source of energy for peripheral tissues. 1 At the cellular level, after being transported through the cell membrane and then into the mitochondria by means of a carnitine-dependent system, long-chain fatty acids are predominantly oxidized in mitochondria. 2 , 3 Common clinical features of disorders of fatty-acid oxidation are metabolic decompensation during fasting, hypoketotic hypoglycemia, and acute dysfunction of fatty-acid–dependent tissues (skeletal muscle, heart, and . . .