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One of the key enzymes utilized in the food industry is pullulanase. But its major drawbacks are its low yield and high production costs. In this regard, the current research aims to screen agro-waste substrates for optimal pullulanase production in solid-state fermentation. Of various agro-wastes used as a substrate, the maximum enzymic activity (9.74 U/gds) was observed in a medium based on 5 g of green gram husk and incubated for 3 days at 30 °C. The effects of 16 different nutrients on the yield of pullulanase production were studied using the Plackett–Burman experimental design. The incorporation of FeSO 4 , MnSO 4 , and MgSO 4 into the pullulanase production medium significantly increased the yield and showed a 5.7-fold increase (56.25 U/gds) in comparison with the unoptimized media. The Box-Behnken experimental design was used to study the effect of interactions between Fe 2+ , Mg 2+ , and Mn 2+ on the production of pullulanase. Box-Behnken showed a 1.1-fold increase (62.1 U/gds) in pullulanase production. The total increase in yield after all optimization was 6.37-fold. The present study reports for the first time the applicability of green gram husk as a potent substrate for pullulanase production by Penicillium viridicatum .

The increase in the global population has led to a substantial increase in the demand for food supply as well as food manufacturing industries that regularly produce large amounts of food waste. Agro-industrial waste has attracted tremendous attention all over the world since ancient times, such waste is usually dumped or burned and poses a threat to human health and the environment, which has always been a matter of serious concern. However, food waste is a major source of complex carbohydrates, proteins, lipids, vitamins, minerals, fibers, and helps in the manufacture of raw materials for a variety of industrial purposes such as the production of biofuels, enzymes, bioactive compounds, biodegradable plastics, surfactants. Hence it is necessary to convert food waste into value-added products that reduce environmental problems. The present review paper attempts to outline and analyze the potential of agro-industrial residues as cost-effective substrates to produce the enzyme amylase using a wide range of microbial strains. Graphical Abstract

Recent advancements in signal processing and computational power have revolutionized computer vision applications in diverse industries such as agriculture, food processing, biomedical, and the military. These developments are propelling efforts to automate processes and enhance efficiency. Notably, computational techniques are replacing labor-intensive manual methods for assessing the maturity indices of fruits and vegetables during critical growth stages. This review paper focuses on recent advancements in computer vision techniques specifically applied to determine the maturity indices of fruits and vegetables within the food processing sector. It highlights successful applications of Nuclear Magnetic Resonance (NMR), Near-Infrared Spectroscopy (NIR), thermal imaging, and image scanning. By examining these techniques, their underlying principles, and practical feasibility, it offers valuable insights into their effectiveness and potential widespread adoption. Additionally, integrating biosensors and AI techniques further improves accuracy and efficiency in maturity index determination. In summary, this review underscores the significant role of computational techniques in advancing maturity index assessment and provides insights into their principles and effective utilization. Looking ahead, the future of computer vision techniques holds immense potential. Collaborative efforts among experts from various fields will be crucial to address challenges, ensure standardization, and safeguard data privacy. Embracing these advancements can lead to sustainable practices, optimized resource management, and progress across industries. Graphical Abstract Highlights 1. Recent advancements in signal processing and computation drive interest in computer vision across industries. 2. The review focuses on non-destructive methods in fruits and vegetables. 3. Computational techniques replace manual methods for maturity index determination. 4. The principles of techniques are highlighted, along with their successful applications. 5. The potential of computation techniques in destructive, non-destructive methods, biosensors, and AI summarized.

Rubus species holds promise as a valuable source of polyphenols and bioactive compounds, offering significant potential as functional food ingredients with both nutraceutical and pharmaceutical benefits. However, many edible species within this genus remain under-explored and their importance is largely unrecognized. This review aims to provide an overview of the nutritional and bioactive components of both explored and under-explored Rubus species, highlighting their potential health advantages, value addition, and recent advancements. The economic exploitation of Rubus is currently limited to a few cultivated species, while numerous non-conventional and wild edible species are overlooked. Recognizing the economic and nutritional significance of exploited Rubus species, it is imperative to explore the untapped potential of these underutilized plants. By doing so, these species can be preserved from endangerment and contribute to nutritional and livelihood security for communities having access to them. This review emphasizes the importance of understanding the exceptional characteristics of Rubus species as \"superfoods\" and encourages the promotion and cultivation of these unexplored species. By expanding the cultivation and utilization of under-explored Rubus species, we can unlock their full potential and support sustainable nutritional and economic benefits.

This study investigates the kinetics and optimization of fluidized bed drying of onion slices using CCRD design by Response Surface Methodology, aiming to enhance drying efficiency and preserve product quality. The effects of drying process parameters viz., drying temperature (43.18, 50,60,70, & 76.82 °C), NaCl concentration (6.59, 10, 15, 20, & 23.41%) and bed thickness (1.636, 3,5, 7, & 8.362 mm) on dehydration ratio, rehydration ratio, color change, ascorbic acid content and overall acceptability were evaluated using multiple response optimization techniques. Optimization analysis revealed that a drying temperature of 70 °C, NaCl concentration of 20%, and bed thickness of 3 mm yielded the highest desirability value of 0.637. Under these optimized conditions, the dehydration ratio, rehydration ratio, color change, ascorbic acid content, and overall acceptability of onion slices were measured at 6.76, 5.87, 4.85, 8.06 and 4.02, respectively. Higher drying temperatures, NaCl concentrations and lower bed thickness were associated with faster drying rates. Various mathematical models, including Page, Logarithmic, Henderson and Pabis, Midilli and Newton models, were employed to characterize the drying kinetics, with the Page Model demonstrating superior fit to experimental data. Furthermore, quality parameters such as moisture content, water activity, ascorbic acid content, color change and crispiness of onion slices stored for three months under optimized conditions and packaged in different materials (LDPE and HDPE) were evaluated. Results indicate that the optimized fluidized bed drying process parameters effectively maintained the quality of onion slices during storage.

The health benefits associated with probiotics have increased their application in pharmaceutical formulations and functional food development. High production of probiotic biomass requires a cost-effective production method and nutrient media optimization. The biomass production of probiotics can be enhanced by optimizing growth parameters such as substrate, pH, incubation time, etc. For economical industrial production of probiotic biomass, it is required to design a new medium with low cost. Wastes from the food industries are promising components for the development of the low-cost medium. Industrial wastes such as cheese whey and corn steep liquor are excellent examples of reliable sources of nitrogen for the biomass production of probiotic bacteria. The increased yield of biomass reduced the cost of production. This review focuses on the importance of probiotic media for biomass production and its challenges. Graphical Abstract

Pullulanases are the most important industrial group of enzymes in family 13 glycosyl hydrolases. They hydrolyze either α-1,6 and α-1,4 or both glycosidic bonds in pullulan as well as other carbohydrates to produce glucose, maltose, and maltotriose syrups, which have important uses in food and other related sectors. However, very less reports are available on pullulanase production from native strains because of low yield issues. In line with the increasing demands for pullulanase, it has become important to search for novel pullulanase-producing microorganisms with high yields. Moreover, high production costs and low yield are major limitations in the industrial production of pullulanase enzymes. The production cost of pullulanase by using the solid-state fermentation (SSF) process can be minimized by selecting agro-industrial waste. This review summarizes the types, sources, production strategies, and potential applications of pullulanase in different food and other related industries. Researchers should focus on fungal strains producing pullulanase for better yield and low production costs by using agro-waste. It will prove a better enzyme in different food processing industries and will surely reduce the cost of products.

An investigation was carried out to evaluate the impact of pretreatments viz. wax coating, hot water dipping, and fungicide treatment on quality characteristics of kinnow. In the present study, a trial was conducted to extend the shelf life of kinnow fruits by maintaining the initial quality characteristics with three wax-to-water dilution ratios (1:1, 1:2, 1:3), hot water dipping at 56 °C with varying time (40, 60, 80 s) along with fungicide treatment at various concentration (150, 200, 250 ppm). Both treated and untreated (experimental control) samples were kept at refrigerated conditions (5–8 °C) and 90–95% RH for 60 days. The effect of the storage period on quality parameters was recorded regarding weight loss, TSS, titrable acidity, ascorbic acid, and total color difference (TCD). The process parameters i.e. wax dilution, hot water dipping time, and fungicide concentration were optimized using response surface methodology (RSM). Optimized process parameters were 1:2.66 waxing dilution, 80 s hot water dipping time, and 250 ppm fungicide concentration. It could be concluded from the results that a combination of wax coating, hot water dipping, and fungicide treatment can help to prolong the storage period of kinnow up to 60 days with retained quality, as compared to 30 days in untreated control kinnow fruits when stored at 5–8 °C and 90–95% relative humidity.

One of the greatest threats to global health is cancer. Probiotic foods have been shown to have therapeutic promise in the management of cancer, even though traditional treatments such as radiation therapy, chemotherapy, and surgery are still essential. The generation of anticarcinogenic compounds, immune system stimulation, and gut microbiota regulation are a few ways that probiotics when taken in sufficient quantities, might help health. The purpose of this review is to examine the therapeutic potential of probiotic foods in the management of cancer. Research suggests that certain strains of probiotics have anticancer effects by preventing the growth of cancer cells, triggering apoptosis, and reducing angiogenesis in new tumors. Probiotics have shown promise in mitigating treatment-related adverse effects, such as diarrhea, mucositis, and immunosuppression caused by chemotherapy, improving the general quality of life for cancer patients. However, there are several factors, such as patient-specific features, cancer subtype, and probiotic strain type and dosage, which affect how effective probiotic therapies are in managing cancer. More research is necessary to find the long-term safety and efficacy characteristics of probiotics as well as to clarify the best ways to incorporate them into current cancer treatment methods. Graphical abstract Graphical representation showing the role of probiotic foods in cancer management.

Endophytic fungi and bacteria were isolated from finger millet and their effects on finger millet growth parameters and zinc and NPK contents in grains were studied. Out of 70 fungal and 112 bacterial endophytes, the two best fungal and bacterial isolates were selected on the basis of zinc solubilization and plant-growth-promoting attributes. The fungal isolates identified were Aspergillus terreus and Lecanicillium sp., and the bacterial isolates were Pseudomonas bijieensis and Priestia megaterium. The endophytic zinc, NPK mobilization, and plant-growth-promoting efficacy were determined in a pot experiment with zinc carbonate as the zinc source. Endophytic-primed plants showed enhanced shoot and root lengths compared to the unprimed control. Endophytes increased the zinc content in grains by between 12.12% and 18.80% compared to control plants. Endophytes also augmented the NPK concentrations in seeds compared to control plants and exhibited stability in a diverse range of pHs, temperatures, and NaCl concentrations, and exhibited growth on various carbohydrate and nitrogen sources. This is the first study reporting the interaction of Aspergillus terreus, Lecanicillium sp., Pseudomonas bijieensis, and Priestia megaterium with finger millet for grain Zn biofortification and NPK concentration enhancement. This study indicated that zinc-dissolving endophytes possess the potential for enhancing the zinc and NPK content in grains in addition to the plant-growth-promoting attributes.