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DspI, a putative enoyl-coenzyme A (CoA) hydratase/isomerase, was proposed to be involved in the synthesis of cis -2-decenoic acid (CDA), a quorum sensing (QS) signal molecule in the pathogen Pseudomonas aeruginosa ( P . aeruginosa ). The present study provided a structural basis for the dehydration reaction mechanism of DspI during CDA synthesis. Structural analysis reveals that Glu126, Glu146, Cys127, Cys131 and Cys154 are important for its enzymatic function. Moreover, we show that the deletion of dspI results in a remarkable decreased in the pyoverdine production, flagella-dependent swarming motility, and biofilm dispersion as well as attenuated virulence in P . aeruginosa PA14. This study thus unravels the mechanism of DspI in diffusible signal factor (DSF) CDA biosynthesis, providing vital information for developing inhibitors that interfere with DSF associated pathogenicity in P . aeruginosa .

A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has not been fixed in the paper.A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has not been fixed in the paper.

In this study, kinetic energy budget equations of rotational and divergent flow in pressure coordinates are derived on terrain-following coordinates. The new formulation explicitly shows the terrain effects and can be applied directly to model-simulated dynamic and ther- modynamic fields on the model＇s original vertical grid. Such application eliminates interpolation error and avoids errors in virtual weather systems in mountainous areas. These advantages and their significance are demonstrated by a numerical study in terrain-following coordinates of a developing vortex after it moves over the Tibetan Plateau in China.

In this paper, a new aromatic diamine monomer 4,4′-(2,6-naphthalenediyl)bis[benzenamine]) (NADA) was synthesized and a series of modified PI films containing naphthalene ring structure obtained by controlling the molar ratio of NADA monomer, ternary polymerization with 4,4′-oxydianiline (ODA), and pyromellitic dianhydride (PMDA). The effects of the introduction of the naphthalene ring on the free volume and various properties of PI were investigated by molecular dynamic simulations. The results show that the comprehensive properties of the modified films are all improved to some extent, with 5% thermal weight loss temperature (Td5%) of 569 °C, glass transition temperature (Tg) of 381 °C, tensile strength of 96.41 MPa, and modulus of elasticity of 2.45 GPa. Dielectric property test results show that the dielectric constant (Dk) of the film at 1 MHz is reduced from 3.21 to 2.82 and dielectric loss (Df) reduced from 0.0091 to 0.0065. It is noteworthy that the PI-1 dielectric constant is reduced from 3.26 to 3.01 at 10 GHz with only 5% NADA doping, which is expected to yield the best ratio and provide the possibility of industrial production.

Spinocerebellar ataxia is a phenotypically and genetically heterogeneous group of autosomal dominant-inherited degenerative disorders. The gene mutation spectrum includes dynamic expansions, point mutations, duplications, insertions, and deletions of varying lengths. Dynamic expansion is the most common form of mutation. Mutations often result in indistinguishable clinical phenotypes, thus requiring validation using multiple genetic testing techniques. Depending on the type of mutation, the pathogenesis may involve proteotoxicity, RNA toxicity, or protein loss-of-function. All of which may disrupt a range of cellular processes, such as impaired protein quality control pathways, ion channel dysfunction, mitochondrial dysfunction, transcriptional dysregulation, DNA damage, loss of nuclear integrity, and ultimately, impairment of neuronal function and integrity which causes diseases. Many disease-modifying therapies, such as gene editing technology, RNA interference, antisense oligonucleotides, stem cell technology, and pharmacological therapies are currently under clinical trials. However, the development of curative approaches for genetic diseases remains a global challenge, beset by technical, ethical, and other challenges. Therefore, the study of the pathogenesis of spinocerebellar ataxia is of great importance for the sustained development of disease-modifying molecular therapies.

To identify risk factors for bacterial intra-abdominal infections (IAIs) after orthotopic liver transplantation (OLT) and establish a nomogram prediction model based on preoperative neutrophil-to-lymphocyte ratio (NLR) and preoperative prothrombin time-international normalized ratio (PT-INR)-to-albumin ratio (PTAR). We retrospectively analyzed 261 patients who received OLT between January 2020 and April 2024 in Beijing Friendship Hospital. Independent risk factors for bacterial IAIs within 30 days postoperatively were identified by logical regression analysis. The area under the receiver operating characteristic (ROC) curve (AUC) was calculated to evaluate the predictive performance of NLR and PTAR as well as the nomogram model based on them. Hepatic function was evaluated using the Child-Turcotte-Pugh (CTP) score and the Model for End-Stage Liver Disease (MELD) score. Fifty patients (19.2%, 50/261) developed postoperative bacterial IAIs. The majority of the 66 isolated strains were gram-negative (63.6%). Most infections (74.0%, 37/50) occurred within two weeks postoperatively. Multivariate logistic regression analysis identified preoperative NLR, preoperative PTAR, acute physiology and chronic health evaluation II (APACHE II) score, and biliary leakage as independent risk factors. A nomogram-based prediction model was constructed utilizing the identified independent risk factors. Internal validation was conducted through the bootstrap method. The Hosmer-Lemeshow test indicated no significant deviation from the goodness-of-fit (χ = 7.349, P = 0.499 > 0.05). The calibration curve indicated excellent concordance between predicted and observed outcomes. The nomogram exhibited strong discriminative capability, evidenced by a C-index of 0.900 (95% CI: 0.828-0.972) and an AUC of 0.912 (95% CI: 0.864-0.960) for predicting abdominal infection, with an optimal diagnostic threshold of 0.221 (95% CI: 0.125-0.265). Decision curve analysis revealed a positive net benefit across a wide range of threshold probabilities (0.03-1.0), indicating the model's substantial clinical utility. Both the preoperative NLR and the preoperative PTAR are independent risk factors for IAIs within 30 days after OLT. The nomogram model based on NLR and PTAR can effectively predict the occurrence of IAIs after OLT.

A new acidly sensitive PEGylated polyethylenimine linked by Schiff base (PEG-s-PEI) was designed to render pH-sensitive PEGylation nanoassemblies through multiple interactions with indomethacin and docetaxel (DTX). DTX nanoassemblies driven by PEG-s-PEI thus formulated exhibited an excellent pH-sensitivity PEGylation cleavage performance at extracellular pH of tumor microenvironment, compared to normal tissues, thereby long circulated in blood but were highly phagocytosed by tumor cells. Consequently, this smart pH-sensitive PEGylation cleavage provided an efficient strategy to target tumor microenvironment, in turn afforded superior therapeutic outcome in anti-tumor activity.

To overcome the limitations of conventional methods, this study developed a novel aerial-ground collaborative framework for multi-dimensional quality assessment of asphalt pavement. The quality inspection of asphalt pavement in the whole construction process is realized. Multiple non-destructive testing (NDT) techniques were integrated, including drone-based infrared thermography, ground-penetrating radar (GPR), and a nuclear-free density gauge. Results showed a strong correlation (R2 > 0.95) between the radar-derived dielectric constant and core samples, enabling rapid, full-coverage characterization. The density gauge achieved less than 3% error. Furthermore, a compactness prediction model based on the dielectric constant and an air void content evaluation model based on temperature parameters are further constructed. This system enables aerial screening, point verification, and ground diagnosis, significantly enhancing inspection efficiency and comprehensiveness.

Background Low-density lipoprotein cholesterol (LDL-C) can increase cardiovascular risk. However, the association between LDL-C change and functional outcomes in acute ischemic stroke (AIS) patients who underwent reperfusion therapy remains unclear. Methods Patients who received reperfusion therapy were consecutively enrolled. LDL-C measurement was conducted at the emergency department immediately after admission and during hospitalization. The change of LDL-C level (ΔLDL-C) was calculated by subtracting the lowest LDL-C among all measurements during hospitalization from the admission LDL-C. Poor functional outcome was defined as modified Rankin Scale (mRS) > 2 at 90 days. Results A total of 432 patients were enrolled (mean age 69.2 ± 13.5 years, 54.6 % males). The mean LDL-C level at admission was 2.55 ± 0.93 mmol/L. The median ΔLDL-C level was 0.43 mmol/L (IQR 0.08–0.94 mmol/L). A total of 263 (60.9 %) patients had poor functional outcomes at 90 days. There was no significant association between admission LDL-C level and functional outcome (OR 0.99, 95 % CI 0.77–1.27, p  = 0.904). ΔLDL-C level was positively associated with poor functional outcome (OR 1.80, 95 % CI 1,12-2.91, p  = 0.016). When patients were divided into tertiles according to ΔLDL-C, those in the upper tertile (T3, 0.80–3.98 mmol/L) were positively associated with poor functional outcomes compared to patients in the lower tertile (T1, -0.91-0.13 mmol/L) (OR 2.56, 95 % CI 1.22–5.36, p  = 0.013). The risk of poor functional outcome increased significantly with ΔLDL-C tertile ( P -trend = 0.010). Conclusions In AIS patients who underwent reperfusion therapy, the decrease in LDL-C level during hospitalization was significantly associated with poor functional outcomes at 90 days.

Peng HB, Liu Y, Hou F, et al. J Inflamm Res. 2026;19:571498. https://doi.org/10.2147/JIR.S571498 On Page 9, Table 5, the average levels of FK506 within 30 days [median (IQR), days, ng/mL] for the Non-Infection Group (n=211) should have been 8.78(6.78,11.85) instead of 5.60(3.18,7.60), for the Infection Group (n=50) should have been 8.60(6.98,10.76) instead of 5.20(3.70, 7.10). The t/Z/χ2 value for the same row should be -0.125 instead of -0.274 and the P value should be 0.90 instead of 0.78. Following internal review, the authors found that incorrect values were transcribed to Table 5. The authors apologize for this error.