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Structural and functional studies on Pseudomonas aeruginosa DspI: implications for its role in DSF biosynthesis
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Journal Article
Structural and functional studies on Pseudomonas aeruginosa DspI: implications for its role in DSF biosynthesis
2018
Overview
DspI, a putative enoyl-coenzyme A (CoA) hydratase/isomerase, was proposed to be involved in the synthesis of
cis
-2-decenoic acid (CDA), a quorum sensing (QS) signal molecule in the pathogen
Pseudomonas aeruginosa
(
P
.
aeruginosa
). The present study provided a structural basis for the dehydration reaction mechanism of DspI during CDA synthesis. Structural analysis reveals that Glu126, Glu146, Cys127, Cys131 and Cys154 are important for its enzymatic function. Moreover, we show that the deletion of
dspI
results in a remarkable decreased in the pyoverdine production, flagella-dependent swarming motility, and biofilm dispersion as well as attenuated virulence in
P
.
aeruginosa
PA14. This study thus unravels the mechanism of DspI in diffusible signal factor (DSF) CDA biosynthesis, providing vital information for developing inhibitors that interfere with DSF associated pathogenicity in
P
.
aeruginosa
.
Publisher
Nature Publishing Group UK,Nature Publishing Group
Subject
/ 64
/ 64/60
/ 692/420
/ 82
/ 82/83
/ Bacterial Proteins - chemistry
/ Bacterial Proteins - genetics
/ Bacterial Proteins - metabolism
/ Biofilms
/ Biofilms - growth & development
/ Enoyl-CoA Hydratase - chemistry
/ Enoyl-CoA Hydratase - genetics
/ Enoyl-CoA Hydratase - metabolism
/ Fatty Acids, Monounsaturated - metabolism
/ Fimbriae, Bacterial - physiology
/ Flagella
/ Gene Expression Regulation, Enzymologic
/ Humanities and Social Sciences
/ Pseudomonas aeruginosa - growth & development
/ Pseudomonas aeruginosa - metabolism
/ Pseudomonas aeruginosa - pathogenicity
/ Science
/ Structure-Activity Relationship
/ Structure-function relationships
/ Swarming
