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Tomato yellow leaf curl virus (TYLCV) is one of the most well-known tomato-infecting begomoviruses and transmitted by Bemisia tabaci . Seed transmission has previously been reported for some RNA viruses, but TYLCV has not previously been described as a seed-borne virus. In 2013 and 2014, without whitefly-mediated transmission, TYLCV was detected in young tomato plants germinated from fallen fruits produced from TYLCV-infected tomato plants in the previous cultivation season. In addition, TYLCV-Israel (TYLCV-IL) was also detected in seeds and their seedlings of TYLCV-infected tomato plants that were infected by both viruliferous whitefly-mediated transmission and agro-inoculation. The seed infectivity was 20–100%, respectively and the average transmission rate to seedlings was also 84.62% and 80.77%, respectively. TYLCV-tolerant tomatoes also produced TYLCV-infected seeds, but the amount of viral genome was less than seen in TYLCV-susceptible tomato plants. When tomato plants germinated from TYLCV-infected seeds, non-viruliferous whiteflies and healthy tomato plants were placed in an insect cage together, TYLCV was detected from whiteflies as well as receiver tomato plants six weeks later. Taken together, TYLCV-IL can be transmitted via seeds and tomato plants germinated from TYLCV-infected seeds can be an inoculum source of TYLCV. This is the first report about TYLCV seed transmission in tomato.

Koi herpesvirus (KHV) is a highly contagious virus that causes high mortality in koi and common carp, leading to a reduction in production worldwide. Recent diagnostic tests based on molecular methods alone (nucleic acid amplification) and indirect immunoassay methods (antibody detection) can be confirmed over KHV infections or prior exposure and latent infections. Unfortunately, there is no established method to detect KHV virus particles, especially when virus titers are low. Therefore, we propose an alternative, direct immunoassay method for viral detection using a single-chain variable fragment (scFv), a specific region of IgG antibodies that binds specifically to KHV particles. The results of functional analyses indicated that four putative scFv candidates, C5, F8, F6, and E4, were specific to KHV, but only F6 and C5 had a high binding affinity. The binding characteristics were confirmed by indirect competitive and sandwich enzyme-linked immunosorbent assays, which indicated that F6 and C5 have a broad penetration area to the binding region and share a similar epitope with commercial KHV monoclonal antibodies. These characteristics were further confirmed by their interactions with purified KHV coat protein by indirect ELISA and Western blot analyses. In conclusion, the F6 and C5 scFvs have adequate binding affinity to KHV particles to permit their use in immunoassays.

Viral hemorrhagic septicemia virus (VHSV), one of the most important viral marine pathogens worldwide, has a broad range of hosts, such as members of the families Salmonidae and Paralichthyidae. In addition to being highly contagious, VHSV causes high lethality. The transmission of VHSV can be both vertical and horizontal. In fish, the resolution of VHSV infection is challenging. Thus, early diagnosis of VHSV infections is critical, especially in fish farms that have a high population of juvenile fish. Serological methods are commonly used to detect viral antigens. However, limited serological methods are available for marine viruses. In this study, a VHSV-specific single-chain variable fragment (scFv), E5, was selected using the yeast surface display and phage display systems. scFv, a type of recombinant antibody, comprises a variable heavy chain (VH) and a variable light chain (VL) connected by a polypeptide linker. An scFv clone was selected from the VHSV glycoprotein-expressing yeast cells using the bio-panning method. The scFv-encoding gene was subcloned and expressed in the Escherichia coli expression system. The binding affinity of the expressed and purified scFv protein was determined using an enzyme-linked immunosorbent assay and western blotting. Thus, this study reported a method to identify VHSV-specific scFv using bio-panning that can be utilized to develop a diagnostic system for other viruses.

Red sea bream iridovirus (RSIV) and Viral hemorrhagic septicemia virus (VHSV ) are the most important viral marine pathogens in South Korea because RSIV and VHSV infect and cause high mortality rates in major fish species such as Paralichthys olivaceus and Sebastes schlegelii . These viruses can be transmitted both vertically and horizontally, and early diagnosis is imperative. In this research, RSIV and VHSV viral genomes are detected by PCR-lateral flow assay (LFA). PCR-LFA is sensitive, capable of detecting a viral genome at a concentration of 2–200 fg/µL. Development of this detection method is very meaningful because LFA is simple, requiring a minimum of personnel training to perform. Additionally, LFA requires less time than other detection methods and can be an immediate detection tool that is indispensable in preventing rapid viral spread.

To commercialize the technology of photoelectrochemical hydrogen production, it is essential to surpass the US. Department of Energy target of 0.36 mmol h −1 cm −2 for 1-sun hydrogen production rate. In this study, we utilize crystalline silicon, which can exhibit the highest photocurrent density (43.37 mA cm −2 ), as the photoelectrode material. However, achieving bias-free water splitting (>1.6 V) remains challenging due to the intrinsic low photovoltage of crystalline silicon (0.6 V). To address this limitation, we replace water oxidation with low-potential furfural oxidation, enabling not only bias-free hydrogen production but also dual hydrogen production at both the cathodic and anodic sides. This approach results in a record 1-sun hydrogen production rate of 1.40 mmol h −1  cm −2 , exceeding the Department of Energy target by more than fourfold. The authors demonstrate a bias-free dual solar hydrogen production system by pairing low-potential furfural oxidation with a Si photocathode, achieving a 1-sun hydrogen production rate that surpasses the Department of Energy target by over four-fold.

Metastable phases—kinetically favoured structures—are ubiquitous in nature 1 , 2 . Rather than forming thermodynamically stable ground-state structures, crystals grown from high-energy precursors often initially adopt metastable structures depending on the initial conditions, such as temperature, pressure or crystal size 1 , 3 , 4 . As the crystals grow further, they typically undergo a series of transformations from metastable phases to lower-energy and ultimately energetically stable phases 1 , 3 , 4 . Metastable phases sometimes exhibit superior physicochemical properties and, hence, the discovery and synthesis of new metastable phases are promising avenues for innovations in materials science 1 , 5 . However, the search for metastable materials has mainly been heuristic, performed on the basis of experiences, intuition or even speculative predictions, namely ‘rules of thumb’. This limitation necessitates the advent of a new paradigm to discover new metastable phases based on rational design. Such a design rule is embodied in the discovery of a metastable hexagonal close-packed (hcp) palladium hydride (PdH x ) synthesized in a liquid cell transmission electron microscope. The metastable hcp structure is stabilized through a unique interplay between the precursor concentrations in the solution: a sufficient supply of hydrogen (H) favours the hcp structure on the subnanometre scale, and an insufficient supply of Pd inhibits further growth and subsequent transition towards the thermodynamically stable face-centred cubic structure. These findings provide thermodynamic insights into metastability engineering strategies that can be deployed to discover new metastable phases. A metastable palladium hydride is synthesized where the unique environment in the liquid cell, namely the limited quantity of Pd precursors and the continuous supply of H, resulted in the formation of the hcp phase.

Phosphatases, together with kinases and transcription factors, are key components in cellular signalling networks. Here, we present a systematic functional analysis of the phosphatases in Cryptococcus neoformans , a fungal pathogen that causes life-threatening fungal meningoencephalitis. We analyse 230 signature-tagged mutant strains for 114 putative phosphatases under 30 distinct in vitro growth conditions, revealing at least one function for 60 of these proteins. Large-scale virulence and infectivity assays using insect and mouse models indicate roles in pathogenicity for 31 phosphatases involved in various processes such as thermotolerance, melanin and capsule production, stress responses, O- mannosylation, or retromer function. Notably, phosphatases Xpp1, Ssu72, Siw14, and Sit4 promote blood-brain barrier adhesion and crossing by C. neoformans . Together with our previous systematic studies of transcription factors and kinases, our results provide comprehensive insight into the pathobiological signalling circuitry of C. neoformans . Phosphatases are key components in cellular signalling networks. Here, the authors present a systematic functional analysis of phosphatases of the fungal pathogen Cryptococcus neoformans , revealing roles in virulence, stress responses, O -mannosylation, retromer function and other processes.

Propylene oxide (PO) is a key industrial chemical, often produced by epoxidizing propylene with H 2 O 2 over titanium silicalite-1. However, current H 2 O 2 production via the anthraquinone process relies on fossil-derived hydrogen, leading to substantial CO 2 emissions. Sustainable PO synthesis requires green H 2 O 2 production. Here, we present a fully unassisted, solar- and bias-free system that generates H 2 O 2 . This platform enables modular, eco-friendly on-site PO synthesis by coupling formaldehyde oxidation with two-electron O 2 reduction under alkaline conditions. Efficient propylene epoxidation under these conditions is achieved using titanium silicalite-1 modified by introducing dinuclear titanium sites with Ti–O–Ti bonds, as revealed by density functional theory and instrumental analyses. The unassisted H 2 O 2 production system is integrated with the modified titanium silicalite-1 to realize continuous PO production (1657 μmol PO  cm −2 over 24 h), without electric or solar energy input. This unassisted PO production method can thus be energy-independent, offering a sustainable alternative to conventional processes. Current propylene oxide production relays on H 2 O 2 generated by the energy-intensive anthraquinone processes. Here, an unassisted H 2 O 2 production system is integrated with modified titanium silicalite-1 to realize continuous propylene oxide production without external energy input.

Background Annexin-A1 (ANXA1) is a glucocorticoid-induced protein with multiple actions in the regulation of inflammatory cell activation. The anti-inflammatory protein ANXA1 and its N-formyl peptide receptor 2 (FPR2) have protective effects on organ fibrosis. However, the exact role of ANXA1 in asthma remains to be determined. The aim of this study was to identify the role of ANXA1 in bronchial asthma. Methods In mice sensitized and challenged with ovalbumin (OVA-OVA mice) and mice sensitized with saline and challenged with air (control mice), we investigated the potential links between ANXA1 levels and bronchial asthma using ELISA, immunoblotting, and immunohistochemical staining. Moreover, we also determined ANXA1 levels in blood from 50 asthmatic patients (stable and exacerbated states). Results ANXA1 protein levels in lung tissue and bronchoalveolar lavage fluid were significantly higher in OVA-OVA mice compared with control mice. FPR2 protein levels in lung tissue were significantly higher in OVA-OVA mice compared with control mice. Plasma ANXA1 levels were increased in asthmatic patients compared with healthy controls. Plasma ANXA1 levels were significantly lower in exacerbated patients compared with stable patients with bronchial asthma ( p  < 0.05). The plasma ANXA1 levels in controlled asthmatic patients were correlated with forced expiratory volume in 1 s (FEV 1 ) ( r  = − 0.191, p  = 0.033) and FEV 1 /forced vital capacity (FVC) ( r  = −0.202, p  = 0.024). Conclusion These results suggest that ANXA1 may be a potential marker and therapeutic target for asthma.

Heat recovery from a high-temperature wastewater is the major concern in the conventional textile industry. However, limited space in the textile plant is an important constraint for the process enhancement. Therefore, an easily applicable heat recovery system with a small amount of additional equipment to the existing dyeing process is required. To meet the needs from the industry, this study suggests an integrated heat recovery and supply system consisting of single heat exchanger and single storage tank using freshwater as a thermal carrier to utilize the reusable heat in the wastewater. Freshwater is stored in a tank after direct heat exchange with wastewater and is supplied to the next dyeing process. Three different designs of the integrated system were compared based on the lower limit of the wastewater temperature: above 50 °C, 40 °C, and 30 °C for Cases 1, 2, and 3, respectively. The energy and energy flow analyses showed Case 2 to be well balanced between the quality and quantity of the recovered heat, and there was no heat loss via drainage. The heat demand for Case 2 was 795.5 kW, which was the lowest among all cases. Furthermore, an economic analysis showed that the total cost for Case 2 was reduced by 63.2% compared with the base case. Despite the use of an additional heat exchanger and water storage tank, the proposed system was more economical because of the reduced operating costs. Finally, a detailed analysis was conducted by determining the more efficient temperature for heat recovery and supply.