Explore the vast range of titles available.

With the rapid development of image processing technology, the application range of image recognition technology is becoming more and more extensive. Processing, analyzing, and repairing graphics and images through computer and big data technology are the main methods to obtain image data and repair image data in complex environment. Facing the low quality of image information in the process of sports, this paper proposes to remove the noise data and repair the image based on the partial differential equation system in image recognition technology. Firstly, image recognition technology is used to track and obtain the image information in the process of sports, and the fourth-order partial differential equation is used to optimize and process the image. Finally, aiming at the problem of low image quality and blur in the transmission process, denoising is carried out, and image restoration is studied by using the adaptive diffusion function in partial differential equation. The results show that the research content of this paper greatly improves the problems of blurred image and poor quality in the process of sports and realizes the function of automatically tracking the target of sports image. In the image restoration link, it can achieve the standard repair effect and reduce the repair time. The research content of this paper is effective and applicable to image processing and restoration.

To address the difficulty frontline commanders face in accurately perceiving fireground risks during the early stages of oil storage tank fires, in this study, we propose a method that integrates complex network theory with a multi-source information fusion approach based on cloud models and Dempster-Shafer (D-S) evidence theory for situational analysis and dynamic perception. Initially, the internal evolution of accident scenarios within individual tanks is modeled as a single-layer network, while scenario propagation between tanks is represented through inter-layer connections, forming a multi-layer complex network for the storage area. The importance of each node is evaluated to assess the risk level of scenario nodes, enabling preliminary situational awareness, with limited reconnaissance information. Subsequently, the cloud model’s capability to handle fuzziness is combined with D-S theory’s strength in fusing multi-source data. Multi-source heterogeneous information is integrated to obtain the confidence levels of key nodes across low, medium, and high-risk categories. Based on these results, high-risk scenarios in oil storage tank emergency response are dynamically adjusted, enabling the updating and prediction of accident evolution. Finally, the proposed method is validated using the 2015 Gulei PX plant explosion case study. The results demonstrate that the approach effectively identifies high-risk scenarios, enhances dynamic situational perception, and is generally consistent with actual accident progression, thereby improving emergency response capability.

Spermidine (Spd) is known to protect macromolecules involved in physiological and biochemical processes in plants. However, it is possible that Spd also plays an osmotic regulatory role in promoting the seed germination of Leymus chinensis ( L. chinensis ) under salt-alkali stress. To investigate this further, seeds of L. chinensis were soaked in Spd solution or distilled water, and a culture experiment was performed by sowing the soaked seeds in saline-alkaline soils. The data showed that the Spd priming resulted in an increase of more than 50% in soluble sugar content and an increase of more than 30% in proline content in the germinating seeds. In addition, the Spd priming resulted in an increase of more than 30% in catalase activity and an increase of more than 25% in peroxidase activity in the germinating seeds and effectively mitigated the oxidative damage to the plasma membrane in the germinating seeds under salt-alkali stress. Moreover, the Spd priming of seeds affected the accumulation of polyamine (PA) and maintained the activities of macromolecules involved in physiological metabolism in germinating seeds exposed to salt-alkali stress. Furthermore, the Spd priming treatment increased the hydrogen peroxide (H 2 O 2 ) level to more than 30% and the Ca 2+ concentration to more than 20% in the germinating seeds, thus breaking the dormancy induction pathways in L. chinensis seeds through beneficial hormone enrichment. This study provides an insight into the Spd-mediated regulation pathway during exogenous Spd priming of L. chinensis seeds, which mitigates osmotic and oxidative damage and maintains the integrality of the cell lipid membrane. Thus, exogenous Spd priming increases PA oxidase activity and maintains the accumulation of H 2 O 2 . We found that the H 2 O 2 beneficially affected the balance of Ca 2+ and hormones, promoting the vigor and germination of L. chinensis in response to salt-alkali stress.

Background Preterm birth is serious public health worldwide, and early prediction of preterm birth in pregnant women may provide assistance for timely intervention and reduction of preterm birth. This study aimed to develop a preterm birth prediction model that is readily available and convenient for clinical application. Methods Data used in this case-control study were extracted from the National Vital Statistics System (NVSS) database between 2018 and 2019. Univariate and multivariate logistic regression analyses were utilized to find factors associated with preterm birth. Odds ratio (OR) and 95% confidence interval (CI) were used as effect measures. The area under the curve (AUC), accuracy, sensitivity, and specificity were utilized as model performance evaluation metrics. Results Data from 3,006,989 pregnant women in 2019 and 3,039,922 pregnant women in 2018 were used for the model establishment and external validation, respectively. Of these 3,006,989 pregnant women, 324,700 (10.8%) had a preterm birth. Higher education level of pregnant women [bachelor (OR = 0.82; 95%CI, 0.81–0.84); master or above (OR = 0.82; 95%CI, 0.81–0.83)], pre-pregnancy overweight (OR = 0.96; 95%CI, 0.95–0.98) and obesity (OR = 0.94; 95%CI, 0.93–0.96), and prenatal care (OR = 0.48; 95%CI, 0.47–0.50) were associated with a reduced risk of preterm birth, while age ≥ 35 years (OR = 1.27; 95%CI, 1.26–1.29), black race (OR = 1.26; 95%CI, 1.23–1.29), pre-pregnancy underweight (OR = 1.26; 95%CI, 1.22–1.30), pregnancy smoking (OR = 1.27; 95%CI, 1.24–1.30), pre-pregnancy diabetes (OR = 2.08; 95%CI, 1.99–2.16), pre-pregnancy hypertension (OR = 2.22; 95%CI, 2.16–2.29), previous preterm birth (OR = 2.95; 95%CI, 2.88–3.01), and plurality (OR = 12.99; 95%CI, 12.73–13.24) were related to an increased risk of preterm birth. The AUC and accuracy of the prediction model in the testing set were 0.688 (95%CI, 0.686–0.689) and 0.762 (95%CI, 0.762–0.763), respectively. In addition, a nomogram based on information on pregnant women and their spouses was established to predict the risk of preterm birth in pregnant women. Conclusions The nomogram for predicting the risk of preterm birth in pregnant women had a good performance and the relevant predictors are readily available clinically, which may provide a simple tool for the prediction of preterm birth.

Calcium (Ca 2+ ) is an essential plant nutrient, and Ca 2+ /H + exchangers (CAXs) regulate Ca 2+ partitioning between subcellular compartments. AtCAX1 activity is inhibited by its N-terminal regulatory region (NRR), which was initially defined as the sequence between the first two methionines. However, the accuracy of this NRR definition and the NRR regulatory mechanism remain unclear. Here, using tomato SlCAX1 as a model, we redefined the NRR of CAXs and demonstrated that our new definition is also applicable to Arabidopsis AtCAX1 and AtCAX3. The N-terminal-truncated SlCAX1 (SlCAX1 Δ39 ) but not the full-length SlCAX1 was active in yeast, similar to Arabidopsis AtCAX1. Characterization of slcax1 mutants generated by CRISPR-Cas9 confirmed the calcium transport ability of SlCAX1. Sequence alignment between SlCAX1, AtCAX1, AtCAX3, and the Bacillus subtilis Ca 2+ /H + antiporter protein YfkE revealed that SlCAX1 does not have the 2nd methionine and YfkE does not have any amino acid residues in front of the first transmembrane domain. Truncating the amino acid residues up to the first transmembrane of SlCAX1 (SlCAX1 Δ66 ) further increased its activity. The same truncation had a similar effect on Arabidopsis AtCAX1 and AtCAX3. Expression of full-length SlCAX1 and SlCAX1 Δ66 in tomato plants confirmed the results. Our results suggest that SlCAX1 is critical for Ca 2+ homeostasis and all the amino acid residues in front of the first transmembrane domain inhibit the activity of CAXs. Our redefinition of the NRR will facilitate fine-tuning of Ca 2+ partitioning to reduce the incidence of Ca 2+ -related physiological disorders in crops.

PurposeManure application affects soil chemical and biochemical properties and organic nitrogen (ON). In this study, we aimed to study the long-term application of manure on soil amino acid pools and the underlying mechanisms that remain unquantified.MethodsWe studied the effects of 41-year manure application on soil ON fractions and amino acid pools during various crop seasons under maize-maize-soybean rotation. This study includes four treatments: no fertilization, inorganic nitrogen + phosphorus + potassium fertiliser (NPK), NPK + 13.5 t ha−1 manure, and NPK + 27 t ha−1 manure (M2NPK).ResultsThe highest values for soil organic carbon (SOC), total nitrogen, pH, urease, and protease activity were observed in both the maize and soybean seasons under M2NPK. The contents of acidolysable ammonium nitrogen, acidolysable amino acid nitrogen (AAN), and ON in soil peaked under M2NPK in both seasons. Long-term high manure additions increased total soil amino acid content by 183% and 174% in 2019 and 2020, respectively. Acidic, alkaline, and neutral amino acid contents peaked during both seasons under M2NPK. Glycine, alanine, proline, lysine, serine, and valine were the predominant amino acid species in the soil, regardless of fertilization. M2NPK treatment led to increases of 88–151%, 79–129%, 70–114%, 87–97%, and 68–72% in the contents of Tyr, Leu, Ile, Glu, and Asp, respectively, compared to NPK. The structural equation modelling (SEM) indicated that SOC, pH, urease, and protease activity were the pathways that manure application alter the AAN pool by collectively accounting for 97% of the AAN variability. Further, our findings from SEM had a direct positive effect of AN on AAN under exogenous N input with a path coefficient of 0.35.ConclusionThe addition of manure can improve soil properties, increase the amino acid pool, and enhance the amino acid pool availability under long-term crop rotation systems. In conclusion, the application of manure represents a practical approach to enhance soil nitrogen fertility and promote sustainable agricultural development.

Anaplastic lymphoma kinase (ALK) rearrangements have been reported in 3-7% of non-small-cell lung cancers (NSCLC). ALK has been reported to be fused with a variety of genes in NSCLC. Significant clinical activity was achieved by ALK inhibitors in patients with NSCLC harbouring ALK translocations. We reported on a 48-year-old male Chinese patient with advanced lung adenocarcinoma harboring a novel ALK-LIMS1 who showed no response to crizotinib. The tissue was assayed by immunohistochemistry (IHC) for ALK and showed diffuse expression of ALK. Next-generation sequencing (NGS) was performed on the peripheral blood and tissue. The previous tumor tissue showed diffuse expression of ALK. Tissue and the later peripheral blood revealed a ALK- LIMS1 fusion. The patient failed to benefit from crizotinib (250 mg, twice a day), with a progression-free survival of two months. We identified a new ALK-LIMS1 fusion from an advanced lung adenocarcinoma which was primary resistant to crizotinib. Our case suggested that the coexistence of mutations and the non-dominant clone, as well as the rearrangement of ALK fusion, did not result in expressed ALK kinase domain that might lead to no response to ALK-TKIs.

Anaplastic lymphoma kinase (ALK) rearrangements have been reported in 3-7% of non-small-cell lung cancers (NSCLC). ALK has been reported to be fused with a variety of genes in NSCLC. Significant clinical activity was achieved by ALK inhibitors in patients with NSCLC harbouring ALK translocations. We reported on a 48-year-old male Chinese patient with advanced lung adenocarcinoma harboring a novel ALK-LIMS1 who showed no response to crizotinib. The tissue was assayed by immunohistochemistry (IHC) for ALK and showed diffuse expression of ALK. Next-generation sequencing (NGS) was performed on the peripheral blood and tissue. The previous tumor tissue showed diffuse expression of ALK. Tissue and the later peripheral blood revealed a ALKLIMS1 fusion. The patient failed to benefit from crizotinib (250 mg, twice a day), with a progression-free survival of two months. We identified a new ALK-LIMS1 fusion from an advanced lung adenocarcinoma which was primary resistant to crizotinib. Our case suggested that the coexistence of mutations and the non-dominant clone, as well as the rearrangement of ALK fusion, did not result in expressed ALK kinase domain that might lead to no response to ALK-TKIs. Keywords: non-small-cell lung cancer, next-generation sequencing, ALK- LIMS1, crizotinib

It is well know that the internal flow field and nozzle geometry affected the spray behavior, but without high-speed microscopic visualization, it is difficult to characterize the spray structure in details. Single-hole Diesel injectors have been used in fundamental spray research, while most direct-injection engines use multi-hole nozzle to tailor to the combustion chamber geometry. Recent engine trends also use smaller orifice and higher injection pressure. This paper discussed the quasi-steady near-nozzle Diesel spray structures of an axisymmetric single-hole nozzle and a symmetric two-hole nozzle configuration, with a nominal nozzle size of 130 μm, and an attempt to correlate the observed structure to the internal flow structure using computational fluid dynamic (CFD) simulation. The test conditions include variation of injection pressure from 30 to 100 MPa, using both Diesel and Biodiesel fuels, under atmospheric condition. The imaging technique utilizes a 150 pico-second synchrotron-based Ultrafast Phase-contrast X-ray in order to freeze-capture the fast moving jet and achieve excellent spatial resolution in order to compare the wavy jet structure. Both nozzle holes originate from a sac of identical geometry, but different flow structure inside the nozzle cause significant difference in the observed flow structure near the nozzle exit. The ultrafast fast images revealed unique surface and internal morphology of the fuel sprays that can be identified. The two-hole nozzle produces much more unstable jet structure under same injection conditions. The early wavelength developed in the jet is measured to be on the order of 30∼80 μm, with the frequency range of 5 to 10 MHz, depending on the injection conditions up to 60 MPa injection pressure when the wavelength analysis is still feasible. The differences between the nozzle configurations are investigated using CFD simulation. The results show that the three-dimensional fluid flow entering the two-hole nozzle generates stronger streamline curvature and stream-wise counter-rotational vortices which are by default absent in the axisymmetric single-hole nozzle. It also produces thicker shear layer and higher turbulence. The interactions of downwash entrance flow with turbulence potentially enhance the instability and produce wider spray cone angles.

In this study, a rat model of inflammatory pain was produced by injecting complete Freund’s adjuvant into the hind paw, and the expression of acetylated histone 3 in the spinal cord dorsal horn was examined using immunohistochemical staining. One day following injection, there was a dramatic decrease in acetylated histone 3 expression in spinal cord dorsal horn neurons. However, on day 7, expression recovered in adjuvant-injected rats. While acetylated histone 3 labeling was present in dorsal horn neurons, it was more abundant in astrocytes and microglial cells. The recovery of acetylated histone 3 expression was associated with a shift in expression of the protein from neurons to glial cells. Morphine injection significantly upregulated the expression of acetylated histone 3 in spinal cord dorsal horn neurons and glial cells 1 day after injection, especially in astrocytes, preventing the transient downregulation. Our results indicate that inflammatory pain induces a transient downregulation of acetylated histone 3 in the spinal cord dorsal horn at an early stage following adjuvant injection, and that this effect can be reversed by morphine. Thus, the downregulation of acetylated histone 3 may be involved in the development of inflammatory pain.