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Purpose Globally, the halal cosmetics market is experiencing rapid growth and is considered a key economic driver in shaping economy development and growth. However, the extant research on halal cosmetics is fragmented, potentially impeding the field’s advancement when challenged with conflicting viewpoints and limited replications. Therefore, this paper aims to address the knowledge gap by conducting a rigorous and technology-enabled systematic review by leveraging appropriate software to comprehensively evaluate the state of the halal cosmetics literature. Design/methodology/approach A domain-based review using a hybrid approach that incorporates both bibliometric and interpretive analyses are used to comprehensively assess the current progress of halal cosmetics, identify research gaps and suggest potential directions for future research. Findings Through a comprehensive review of 66 articles, this review provides a holistic and comprehensive overview of halal cosmetics that both academic scholars and market practitioners can rely upon in strategizing and positioning for future development of halal cosmetics. The study provides a holistic and comprehensive overview of halal cosmetics that both academic scholars and market practitioners can reply upon in strategizing and positioning for future development of halal cosmetics. Originality/value The fragmented knowledge of extant research on halal cosmetics across various disciplines limits a comprehensive understanding of the field. It is opportune to conduct a comprehensive and systematic review of the field, providing insight into both its current and future progress. In this regard, this review serves as a “one-stop reference” in providing a state-of-the-art understanding of the field, and enables industry practitioners to reveal the full potential and bridge the theory-practice gap in the halal cosmetics industry.

We developed an automated high-throughput Smart-seq3 (HT Smart-seq3) workflow that integrates best practices and an optimized protocol to enhance efficiency, scalability, and method reproducibility. This workflow consistently produces high-quality data with high cell capture efficiency and gene detection sensitivity. In a rigorous comparison with the 10X platform using human primary CD4 + T-cells, HT Smart-seq3 demonstrated higher cell capture efficiency, greater gene detection sensitivity, and lower dropout rates. Additionally, when sufficiently scaled, HT Smart-seq3 achieved a comparable resolution of cellular heterogeneity to 10X. Notably, through T-cell receptor (TCR) reconstruction, HT Smart-seq3 identified a greater number of productive alpha and beta chain pairs without the need for additional primer design to amplify full-length V(D)J segments, enabling more comprehensive TCR profiling across a broader range of species. Taken together, HT Smart-seq3 overcomes key technical challenges, offering distinct advantages that position it as a promising solution for the characterization of single-cell transcriptomes and immune repertoires, particularly well-suited for low-input, low-RNA content samples.

Background Prompt diagnosis and treatment are essential for malaria control but are typically assessed only among children under five. Older children and adults also experience malaria and may act as reservoirs for transmission but barriers such as distance to facilities, supply shortages, and mistrust of the health system limit access to care across age groups. This study aimed to determine how demographic and parasitological factors influence treatment-seeking behaviour over time. Methods A community-based, open-enrollment longitudinal cohort study was conducted in rural Malawi from April 2019 to May 2020. Data were collected during monthly scheduled active case detection (ACD) visits and passive case detection (PCD) visits at local health centres. Malaria-related treatment-seeking behaviour was assessed using two outcomes: (1) self-reported treatment-seeking between ACD visits and (2) attendance at study-linked health centres during illness. Treatment-seeking was categorized as occurring in the formal or informal sectors. Mixed-effects logistic regression models were used to identify predictors of formal-sector treatment-seeking and health centre attendance, adjusting for clustering at the individual and household levels. Poisson regression was used to estimate rate ratios of PCD visits per person-year. Results The study enrolled 962 participants who contributed 7293 total visits, including 6794 ACD and 499 PCD visits. Most reported treatment-seeking (82%, 720/880 visits) occurred in the formal health sector and longer walking time and higher household head education were associated with reduced odds of formal treatment-seeking (OR = 0.28 for > 90 min vs. < 30 min; OR = 0.41 for secondary education vs. no education). In longitudinal models, older age, male sex, and greater distance to the facility were consistently associated with lower odds of attending PCD visits. Fever was positively associated with subsequent PCD visits (OR = 1.60(1.16–2.21)), but Plasmodium falciparum infection and parasite density were not. The overall PCD visit rate was 70 per 100 person-years and was significantly lower among older children, adults, males, and households farther from health facilities. Conclusions Despite free access to malaria care, barriers to treatment persist. These findings highlight the need for more equitable, community-based approaches to malaria diagnosis and treatment across all age groups.

Introduction: Indigenous communities grapple with unique challenges in the aging process, often encountering amplified barriers to healthcare and resources, possibly due to their remote locations and distinct cultural backgrounds. Limited research exists on aging within Costa Rica’s Ngäbe‑Buglé Indigenous community, particularly the La Casona community in Coto Brus. This study explores the aging experience of the Ngäbe‑Buglé community in La Casona, aiming to identify challenges and potential resources to enhance the quality of life and aging experience for elderly community members. Methods: This qualitative study utilized semi‑structured interviews to collect data from 14 elderly participants (6 female, 8 male), aged 52–90 years, living in the La Casona community. Participants were selected through purposeful snowball sampling techniques, and individual interviews were conducted at their residences. Interviews were conducted with assistance from an interpreter and lasted approximately 30–60 minutes. Thematic analysis was used to examine participants’ responses to understand their experiences with aging. Results: Three primary themes emerged: economic difficulties, insufficient social support, and cultural aspects related to La Casona. Among the three primary themes were a total of eight subcategories. Economic challenges encompassed financial constraints and food insecurity, housing and infrastructure needs, and difficulties in accessing healthcare. Insufficient social support was evident through heavy reliance on family, limited community aid, and an absence of engaging activities. Cultural aspects highlighted the community’s deep connection to nature and concerns about the fading cultural heritage among younger generations. These themes collectively contribute to the challenges confronted by elderly adults in the Ngäbe‑Buglé community. Conclusions: Improving healthcare access, enhancing social interactions, and preserving cultural heritage are essential when it comes to improving the aging experience in La Casona. The following participant discussions provide insight into public health interventions. Addressing these issues will require governmental support and policy changes aimed at uplifting the Ngäbe‑Buglé community.

Introduction: As trends in life expectancy continue to improve, the burden of loneliness in geriatric populations on a global scale is increasing. With advancing age comes an increase in the number of life events that can perpetuate a state of loneliness such as losing a life partner, dwindling social networks, and deteriorating health conditions. This burden can manifest in a variety of mental and physical consequences. While loneliness has been studied in a few communities around the world, there is a need to study loneliness in the context of Latin American communities in Central America, including Costa Rica. The aim of the present study is to assess the prevalence and associated factors of social and emotional loneliness (SEL) in a sample of elderly patients in the canton of Coto Brus, Costa Rica. Methods: A cross‑sectional study was conducted that sampled 63 adults aged 65 years or above in the canton of Coto Brus. Investigators conducted face‑to‑face interviews in Spanish with the aid of translators. The primary instruments used for the present study were a content‑validated version of the 11‑item De Jong Gierveld Loneliness Scale and socio‑demographic questions that included age, sex, address, civil status, and level of education. Results: A high degree of SEL was found, with 60.3% of participants noting at least a moderate degree of loneliness, with the average score being 3.33 on the 11‑point scale. When SEL was broken up into its respective subscores, the average score for social loneliness (SL) was found to be 0.67 on the 5‑point scale, and the average score for emotional loneliness (EL) was found to be 2.67 on the 6‑point scale. There was also evidence that supports both level of education and marital status serving as protective factors in the development of SEL. Discussion: These results could indicate a stronger association of loneliness being linked to missing a life partner compared with loneliness being linked to having smaller social networks. Given the associations that were found in this preliminary study, it is pivotal to explore loneliness in this community with a larger sample size—potentially through the integration of the country’s Equipos Básicos de Atención Integral de Salud (EBAIS) healthcare system. It is also crucial to expand the study to explore any associations between loneliness and comorbid mental and physical health conditions.

Using pre-miR-451 as a model molecule, we have characterized the general molecular properties of small hairpin RNAs that are processed into potent small interfering RNAs (siRNA) by Argonaute2 (Ago2). The Ago2-sliced siRNAs (sli-siRNAs) have the same silencing potency as the classical Dicer diced siRNAs (di-siRNAs) but have dramatically reduced unwanted sense strand activities. We have built vectors with the constitutive or inducible U6 promoter that can express sli-siRNAs in mammalian cells, in which the sli-siRNAs can be correctly processed to repress target genes. As a proof of principle for potential applications of sli-siRNAs in vivo, we show that the expression of one Ago2 shRNA-1148 in HCT-116 colon cancer cells knocked down RRM2 expression and reduced the proliferation and invasiveness of the cells. The defined sli-siRNA model molecules and the expression systems established in this study will facilitate the design and application of sli-siRNAs as novel potent RNAi triggers with reduced off-target effects.

Part one:The earlier years I am Christine, Ray’s younger sister. The last time that I was in this auditorium (Kendal re-tirement community), about 7 years ago, Ray was giving a wonderful talk on "CHINA". Ray was always very proud of his Chinese heritage, especially since he was born and raised in China where he lived twenty years.

Background Chronic low back pain (CLBP) is the most common chronic pain condition and is often resistant to conventional treatments. Acupuncture is a popular alternative for treating CLBP but its mechanisms of action remain poorly understood. Evidence suggests that pain regulatory mechanisms (particularly the ascending and secondarily the descending pain modulatory pathways) and psychological mechanisms (e.g., expectations, pain catastrophizing and self-efficacy) may be involved in the pathogenesis of CLBP and its response to treatments. We will examine these mechanisms in the treatment of CLBP by electroacupuncture (EA). Methods We present the aims and methods of a placebo-controlled, participant-blinded and assessor-blinded mechanistic study. Adult patients with CLBP will be randomized to receiving 16 sessions of real (active) or sham (placebo) EA over the course of 8 weeks. The primary pain regulatory measure for which the study was powered is temporal summation (TS), which approximates ascending pain facilitation. Conditioned pain modulation (CPM), representing a descending pain modulatory pathway, will be our secondary pain regulatory measure. The primary psychological measure is expectations of benefit, and the secondary psychological measures are pain catastrophizing and self-efficacy in managing pain. Main clinical outcomes are back pain bothersomeness on a 0–100 visual analog scale (primary), Roland Morris Disability Questionnaire (secondary), and relevant items from the National Institutes of Health (NIH) Patient-Reported Outcome Measures Information System (secondary). We hypothesize that compared to sham, real EA will lead to greater reduction in TS after 8 treatment sessions (4 weeks); and that reduction in TS (and secondarily, increase in CPM) after 8 treatment sessions will mediate reduction in back pain bothersomeness from baseline to week 10 (clinical response) to EA. We also hypothesize that the three psychological factors are moderators of clinical response. With 100 treatment completers, the study is designed to have 80% power to detect a medium-sized between-group effect ( d  = 0.5) on temporal summation. Discussion To the best of our knowledge, this is the first appropriately powered, placebo-controlled clinical trial evaluating mechanisms of EA in the treatment of CLBP. Trial registration ClinicalTrials.gov, NCT02503475 . Registered on 15 July 15 2015. Retrospectively registered.

The use of face masks or respirators (N95/KN95) is recommended to reduce transmission of SARS-CoV-2, the virus that causes COVID-19 (1). Well-fitting face masks and respirators effectively filter virus-sized particles in laboratory conditions (2,3), though few studies have assessed their real-world effectiveness in preventing acquisition of SARS-CoV-2 infection (4). A test-negative design case-control study enrolled randomly selected California residents who had received a test result for SARS-CoV-2 during February 18-December 1, 2021. Face mask or respirator use was assessed among 652 case-participants (residents who had received positive test results for SARS-CoV-2) and 1,176 matched control-participants (residents who had received negative test results for SARS-CoV-2) who self-reported being in indoor public settings during the 2 weeks preceding testing and who reported no known contact with anyone with confirmed or suspected SARS-CoV-2 infection during this time. Always using a face mask or respirator in indoor public settings was associated with lower adjusted odds of a positive test result compared with never wearing a face mask or respirator in these settings (adjusted odds ratio [aOR] = 0.44; 95% CI = 0.24-0.82). Among 534 participants who specified the type of face covering they typically used, wearing N95/KN95 respirators (aOR = 0.17; 95% CI = 0.05-0.64) or surgical masks (aOR = 0.34; 95% CI = 0.13-0.90) was associated with significantly lower adjusted odds of a positive test result compared with not wearing any face mask or respirator. These findings reinforce that in addition to being up to date with recommended COVID-19 vaccinations, consistently wearing a face mask or respirator in indoor public settings reduces the risk of acquiring SARS-CoV-2 infection. Using a respirator offers the highest level of personal protection against acquiring infection, although it is most important to wear a mask or respirator that is comfortable and can be used consistently.

Harmful alcohol consumption can significantly compromise adherence to antiretroviral therapy (ART). Prior research has identified aggregate relationships between alcohol use and ART non-adherence, largely relying on concurrent assessment of these domains. There is relatively limited evidence on more nuanced day-level associations between alcohol use and ART non-adherence, despite potentially important clinical implications. We recruited adults with HIV treatment adherence challenges and harmful alcohol use (n = 53) from HIV care in South Africa. We examined relationships between alcohol use and same and next day ART adherence, accounting for the role of weekends/holidays and participant demographics, including gender. Results demonstrated that ART adherence was significantly worse on weekend/holiday days. Next day adherence was significantly worse in the context of weekend alcohol use and among men. These results suggest the importance of tailoring intervention strategies to support ART adherence during weekend drinking and for men engaged in heavy episodic drinking.