Asset Details
Do you wish to reserve the book?
Single-cell sequencing of full-length transcripts and T-cell receptors with automated high-throughput Smart-seq3
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Single-cell sequencing of full-length transcripts and T-cell receptors with automated high-throughput Smart-seq3
Do you wish to request the book?
Single-cell sequencing of full-length transcripts and T-cell receptors with automated high-throughput Smart-seq3
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Single-cell sequencing of full-length transcripts and T-cell receptors with automated high-throughput Smart-seq3
2024
Overview
We developed an automated high-throughput Smart-seq3 (HT Smart-seq3) workflow that integrates best practices and an optimized protocol to enhance efficiency, scalability, and method reproducibility. This workflow consistently produces high-quality data with high cell capture efficiency and gene detection sensitivity. In a rigorous comparison with the 10X platform using human primary CD4 + T-cells, HT Smart-seq3 demonstrated higher cell capture efficiency, greater gene detection sensitivity, and lower dropout rates. Additionally, when sufficiently scaled, HT Smart-seq3 achieved a comparable resolution of cellular heterogeneity to 10X. Notably, through T-cell receptor (TCR) reconstruction, HT Smart-seq3 identified a greater number of productive alpha and beta chain pairs without the need for additional primer design to amplify full-length V(D)J segments, enabling more comprehensive TCR profiling across a broader range of species. Taken together, HT Smart-seq3 overcomes key technical challenges, offering distinct advantages that position it as a promising solution for the characterization of single-cell transcriptomes and immune repertoires, particularly well-suited for low-input, low-RNA content samples.
Publisher
BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC
Subject
/ Animal Genetics and Genomics
/ Animals
/ Biomedical and Life Sciences
/ CD4-Positive T-Lymphocytes - metabolism
/ Genes
/ Genomics
/ High-Throughput Nucleotide Sequencing - methods
/ Humans
/ Methods
/ Microbial Genetics and Genomics
/ Reagents
/ Receptors, Antigen, T-Cell - genetics
/ Single-Cell Analysis - methods
/ T cells
/ Workflow
