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Introduction The transgenic murine Cre/loxP system is deployed to investigate the role of central nervous system (CNS) cell‐specific gene alterations in both healthy conditions and models of neurologic disease. The Aldh1l1‐Cre/ERT2 line is widely used to target astrocytes with high coverage and specificity within the CNS. Specificity outside the CNS, however, has not been well‐characterized, and Aldh1l1‐Cre/ERT2‐mediated recombination within the spleen has been reported. In many CNS diseases, infiltrating immune cells from the periphery drive or regulate pathogenesis. We tested whether flox‐mediated recombination from Aldh1l1‐Cre/ERT2 occurs in immune cells in addition to astrocytes and whether these cells traffic from the spleen into the spinal cord during experimental autoimmune encephalomyelitis (EAE), a model of CNS autoimmune disease. Methods Two astrocyte‐targeted mouse lines were generated with the red fluorescent reporter, tdTomato, by crossing the Cre‐recombinase lines, Tg(Aldh1l1‐Cre/ERT2)1Khakh and Tg(Gfap‐Cre)73.12Mvs, with the reporter line, Gt(ROSA)26Sor. Aldh1l1‐Cre/ERT2 was activated with 5 days of intraperitoneal tamoxifen, whereas Gfap‐Cre was constitutively active. EAE was induced 2 weeks after tamoxifen, and then spleens and spinal cords were harvested and processed for flow cytometry at various time points after disease onset in EAE versus healthy controls. Results In EAE, Aldh1l1‐Cre/ERT2, but not Gfap‐Cre, induced multiple tdTomato+ immune cell subpopulations in the spleen and spinal cord, including macrophages, monocytes, neutrophils, eosinophils, B cells, CD4+, and CD8+ T cells. Conclusion Use of Aldh1l1‐Cre/ERT2 should therefore account for recombination in both astrocytes and immune cells in disease models involving peripheral immune cell infiltration into the CNS.

This article develops an analytical framework for mission command; proposes the full age of sail as an area for current military officers to mine for relevant lessons; and examines, through the mission-command lens, a case study from that era, involving a dispute between the British naval and land-force commanders in the Caribbean during the Hundred Days of Napoléon Bonaparte.

Company leaders can do this by listing potential deepfakes thai could damage Iheii reputations and then create an appropriate company response foi each one in case the incident actually happens. Some examples of damaging deepfakes could be scenarios that include; security risks for homeowners, damage to property, misrepresentation of before and after results or fake testimonials or reviews. Additionally, many of these companies restrict their products to government or university research, while others focus on enterprise clients, like insurance companies, for fraud identification.

[...]many do say it’s operating on a nearly empty tank of original imagination with the seemingly endless stream of sequels, prequels and reboots that studios crank out every year with the streamlined efficiency of an early Ford assembly line. The latest to attempt to turn TV history into box office gold is “CHiPs,” a surprising laugh-out-loud R-rated comedy that, while following in a similar vein of “Jump Street,” also ups the ante on the entire trend of turning shows into movies. Based on the dramatic series of the same name (about motorcycle-riding California Highway policemen that made a star out of Eric Estrada and ran from 1977 to 1983), “CHiPs” was written and directed by Dax Shepard (“Idiocracy,” “Let’s Go To Jail”) who also stars in it and doesn’t waste any time getting to the punchlines. In “CHiPs,” Baker (Shepard) is a pill-munching shlub with bad knees who only joins the CHP to win back the affections of his apathetic swim-instructing wife, played by his actual real-life wife, Kristen Bell. Shepard also made the insanely smart move of casting Michael Pena as Ponch, who is actually an undercover FBI agent sent to join the CHP as part of an operation to root out some dirty cops who have been committing literal highway robbery. Specifically, Whitlock Jr. is terrific as Ponch’s exasperated boss — another example of turning the serious into light-heartedness — who can’t get over the exorbitant amount of federal money being spent on the operation, not to mention...

[...]after nearly two decades of playing such a pivotal comic book role that predates both the Marvel Cinematic Universe and the DC Extended Universe, Jackman is hanging up his bloodied wife beater and closing the box of cigars that is this chapter of his acting career with “Logan” (premieres March 3), the swan song of a character who has endured for so long in the annals of history and on the silver screen. In this future, the X-Men are no more (the original comics of their exploits exist, but are simply exaggerations of real-world events), Professor X (Patrick Stewart in a particularly vulnerable performance that would put James McAvoy to shame) is having horrible psychic seizures in a rusting Mexican hideout and Wolverine (aka James Howlett) is now making a living as a limo driver. Yes, Laura (or X-23) is his daughter and the circumstances surrounding her upbringing are like something out of “The Boys From Brazil” and its setup was a quick, little you-already-left-the-theater-to-go-pee post-credits scene after this past summer’s “X-Men Apocalypse.” If the “Guardians of the Galaxy” (their sequel coming out in May) are the neighborhood kids playing stickball outside, then Wolverine is the crotchety old man telling...

[...]Wentworth has lost the ability to capture love in just a few words, reduced to reliving the good old days in the break room of the greeting card company he works for, AAAAA, with a ragtag group of amateurs played by — and I kid you not — Flo from the Progressive commercials and Ryan McPoyle from “It’s Always Sunny in Philadelphia.” When the California governor announces the movie’s eponymous new holiday to help give the greeting card industry “a boost,” Ray, now fired and spending his days watching homeless people fight on TV, is hired by his old boss to write one more card for the newly-christened occasion. What ensues is like J.J. Gittes browsing the greeting card aisle in a pharmacy greeting as Ray gets himself caught up in a morally dubious meshwork of murder, MacGuffins, lies, femme fatales and crooked cops set to the mainstay sound of...

[...]the animated DC universe is alive and well, allowing Warner to rake in the box office profits via its animation division before setting sail for stranger tides with film adaptations of in-house Lego brands like “The Lego Ninjago Movie,” which is set for a release this September. The characters know they’re ageless pawns in a vast mythological well that has quenched the thirsts of legendary comic book writers like Alan Moore and Neil Gaiman. Like the beautiful and mock stop motion animation style, the movie’s jokes and cameos come fast and heavy as Batman attempts to banish Joker to the Phantom Zone, which only unleashes an evil rogues gallery of pop culture history’s worst baddies — this is where the true might and reach of Lego comes into play with some truly bizarre yet epic appearances. In the Lego cinematic universe, these two things aren’t mutually exclusive, but have a symbiotic relationship that keeps the kids entertained with the flashy colors and mile-a-minute jokes while simultaneously winking to the adults in the audience with nostalgic references to their own upbringings. [...]the movie honors its hero’s extensive source material by having the brazen Joker-esque ability to laugh at itself, scars and...