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Class I KNOTTED-like homeobox ( KNOXI ) genes are parts of the regulatory network that control the evolutionary diversification of leaf morphology. Their specific spatiotemporal expression patterns in developing leaves correlate with the degrees of leaf complexity between simple-leafed and compound-leafed species. However, KNOXI genes are not involved in compound leaf formation in several legume species. Here, we identify a pathway for dual repression of MtKNOXI function in Medicago truncatula . PINNATE-LIKE PENTAFOLIATA1 ( PINNA1 ) represses the expression of MtKNOXI , while PINNA1 interacts with MtKNOXI and sequesters it to the cytoplasm. Further investigations reveal that UNUSUAL FLORAL ORGANS ( MtUFO ) is the direct target of MtKNOXI, and mediates the transition from trifoliate to pinnate-like pentafoliate leaves. These data suggest a new layer of regulation for morphological diversity in compound-leafed species, in which the conserved regulators of floral development, MtUFO , and leaf development, MtKNOXI , are involved in variation of pinnate-like compound leaves in M. truncatula . This study reveals a pathway in which the transformation of trifoliate leaves into pinnate-like pentafoliate leaves is regulated by the conserved regulators of floral development ( MtUFO ) and leaf development ( MtKNOXI ) in M. truncatula .

TCP proteins, the plant-specific transcription factors, are involved in the regulation of multiple aspects of plant development among different species, such as leaf development, branching, and flower symmetry. However, thus far, the roles of TCPs in legume, especially in nodulation are still not clear. In this study, a genome-wide analysis of genes was carried out to discover their evolution and function in . In total, 21 were identified and classified into class I and class II, and the class II were further divided into two subclasses, CIN and CYC/TB1. The expression profiles of are dramatically different. The universal expression of class I was detected in all organs. However, the in CIN subclass were highly expressed in leaf and most of the members in CYC/TB1 subclass were highly expressed in flower. Such organ-specific expression patterns of suggest their different roles in plant development. In addition, most were down-regulated during the nodule development, except for the putative targets, , and . Overexpression of significantly reduced the expression level of and resulted in the decreased nodule number, indicating the important roles of -targeted in nodulation. Taken together, this study systematically analyzes the gene family at a genome-wide level and their possible functions in nodulation, which lay the basis for further explorations of module in association with nodule development in .

Background SQUAMOSA Promoter Binding Protein-Likes (SPLs) proteins are plant-specific transcription factors that play many crucial roles in plant growth and development. However, there is little information about SPL family in the model legume Medicago truncatula . Results In this study, a total of 23 MtSPL genes were identified in M. truncatula genome, in which 17 of the MtSPLs contained the putative MtmiR156 binding site at the coding or 3′ UTR regions. Tissue-specific expression pattern analysis showed that most MtmiR156 -targeted MtSPLs were highly expressed in seed and pod. The observation of MtmiR156B -overexpressing plants reveals that MtmiR156/MtSPL modules are not only involved in the development of leaves and branches, but also in the seed pod development, especially the formation of spine on pod. Conclusion The spines on pods are developed in many plant species, which allow pods to adhere to the animals, and then be transported on the outside. This study sheds light on the new function of SPL family in seed dispersal by controlling the formation of spiky pod, and provides insights on understanding evolutionary divergence of the members of SPL gene family among plant species.

Nitrogen is an indispensable nutrient for plant growth and crop production, but it is not directly accessible to plants without the help of nitrogen-fixing bacteria. Legume plants can form root nodules in symbiosis with rhizobia. NODULE INCEPTION (NIN), a founding member of the NIN-like protein (NLP) family, is essential for nodulation in legume species. However, the knowledge of functional characteristics of the NLP family members in peanuts is limited. In this study, a genome-wide analysis of the NLP genes was carried out. A total of 16 NLP genes were identified in the peanut genome, including 2 AhNIN and 14 AhNLP, which were unevenly distributed on nine chromosomes of the peanut genome. Furthermore, transcriptomic profiles and expression pattern analysis showed that both AhNINa and AhNINb genes were specifically expressed in root nodules. Subcellar localization and transcriptional activity analysis revealed that both AhNINa and AhNINb encode transcriptional activators. In addition, the roots that down-regulated the expression of AhNINa and AhNINb genes failed to form nodules. These findings provide significant insights into the molecular functions of AhNINa and AhNINb genes in regulating peanut nodule development.

Under the guidance of carbon peaking and carbon neutrality goals, the power industry is transitioning toward environmentally friendly practices. With the increasing integration of intermittent renewable energy sources (RES) and the enhanced self-regulation capabilities of grids, traditional distribution networks (DNs) are transitioning into active distribution networks (ADNs). To fully exploit the synergistic optimization potential of the “source-grid-load-storage” system in electricity-carbon coupling scenarios, leverage user-side flexibility resources, and facilitate low-carbon DN development, this paper proposes a low-carbon optimal scheduling strategy for ADN incorporating demand response (DR) priority. Building upon a bi-directional feedback mechanism between carbon potential and load, a two-layer distributed robust scheduling model for DN is introduced, which is solved through hierarchical iteration using column and constraint generation (C&CG) algorithm. Case study demonstrates that the model proposed in this paper can effectively measure the priority of demand response for different loads. Under the proposed strategy, the photovoltaic (PV) consumption rate reaches 99.76%. Demand response costs were reduced by 6.57%, and system carbon emissions were further reduced by 8.93%. While accounting for PV uncertainty, it balances the economic efficiency and robustness of DN, thereby effectively improving system operational safety and reliability, and promoting the smooth evolution of DN toward a low-carbon and efficient operational mode.

Haglund's deformity (a prominence in the posterosuperior aspect of the calcaneum) is a known cause of posterior heel pain. Surgery is reserved for patients after failed conservative treatment. Zadek osteotomy is a dorsal-closing wedge osteotomy that reduces the posterior heel prominence. Zadek osteotomy is becoming a favored procedure, however, there are still relatively few studies focusing on patient-reported outcomes. Our main aim was to assess patient-reported outcomes following the Zadek osteotomy in refractory Haglund's deformity. Our secondary aim was to evaluate the correlation between patient outcomes and changes in their pre and postoperative Fowler-Philip and calcaneal pitch angles. We conducted a retrospective review of 19 patients (20 heels) who underwent Zadek osteotomy by a single surgeon at a tertiary hospital over six years. Patient-reported outcomes were collected preoperatively and at 12 months postoperatively using the validated Manchester-Oxford foot questionnaire (MOXFQ) scoring system. We also calculated the difference in their pre and postoperative Fowler-Philip angles and calcaneal pitch using the picture archiving communication system. There was an average improvement of 108 points in the MOXFQ score at 12 months (P<0.05). There was no statistically significant change in calcaneal pitch. However, the Fowler-Phillip angle dropped with an average of 11.4 º (P<0.05). A decrease in the Fowler-Philip angle does improve patient-related outcome measurement scores, however, the relationship is not directly proportional with \"r\" measured at 0.23. Our results show that Zadek osteotomy is a useful procedure to consider in patients with symptomatic refractory Haglund's deformity, with an improvement in patient outcomes at 12 months. However, further studies are needed to give stronger evidence for the efficacy of this procedure and its radiological correlations.

Leaves are the primary photosynthetic structures, while photosynthesis is the direct motivation of crop yield formation. As a legume plant, peanut ( Arachis hypogaea ) is one of the most economically essential crops as well as an important source of edible oil and protein. The leaves of A. hypogaea are in the tetrafoliate form, which is different from the trifoliate leaf pattern of Medicago truncatula , a model legume species. In A . hypogaea , an even-pinnate leaf with a pair of proximal and distal leaflets was developed; however, only a single terminal leaflet and a pair of lateral leaflets were formed in the odd-pinnate leaf in M. truncatula . In this study, the development of compound leaf in A. hypogaea was investigated. Transcriptomic profiles revealed that the common and unique differentially expressed genes were identified in a proximal leaflet and a distal leaflet, which provided a research route to understand the leaf development in A. hypogaea . Then, a naturally occurring mutant line with leaf developmental defects in A. hypogaea was obtained, which displayed a pentafoliate form with an extra terminal leaflet. The characterization of the mutant indicated that cytokinin and class I KNOTTED-LIKE HOMEOBOX were involved in the control of compound leaf pattern in A. hypogaea . These results expand our knowledge and provide insights into the molecular mechanism underlying the formation of different compound leaf patterns among species.

WUSCHEL (WUS) is thought to be required for the establishment of the shoot stem cell niche in . ( ), a gene that encodes a WUS-related homeobox family transcription factor, is thought to be the ortholog of the gene. plays conserved roles in shoot apical meristem (SAM) and axillary meristem (AM) maintenance. is also involved in compound leaf morphogenesis in ; however, its regulatory mechanism has not yet been explored. Here, the significance of in leaf development was investigated. Unlike in , was transcribed not only in the SAM and AM but also in the leaf. Both the patterning of the compound leaves and the shape of the leaf margin in mutant were abnormal. The transcriptional profile of the gene , which encodes an auxin efflux carrier, was impaired and the plants' auxin response was compromised. Further investigations revealed that positively regulated auxin response likely through the recruitment of MtTPL/MtTPRs into the HDL repressor complex. Its participation in auxin-dependent compound leaf morphogenesis is of interest in the context of the functional conservation and neo-functionalization of the products of orthologs.

Formation of nodules on legume roots results from symbiosis with rhizobial bacteria. Here, we identified two GATA transcription factors, MtHAN1 and MtHAN2 , in Medicago truncatula , which are the homologs of HANABA TARANU ( HAN ) and HANABA TARANU LIKE in Arabidopsis thaliana . Our analysis revealed that MtHAN1 and MtHAN2 are expressed in roots and shoots including the root tip and nodule apex. We further show that MtHAN1 and MtHAN2 localize to the nucleus where they interact and that single and double loss-of-function mutants of MtHAN1 and MtHAN2 did not show any obvious phenotype in flower development, suggesting their role is different than their closest Arabidopsis homologues. Investigation of their symbiotic phenotypes revealed that the mthan1 mthan2 double mutant develop twice as many nodules as wild type, revealing a novel biological role for GATA transcription factors. We found that HAN1/2 transcript levels respond to nitrate treatment like their Arabidopsis counterparts. Global gene transcriptional analysis by RNA sequencing revealed different expression genes enriched for several pathways important for nodule development including flavonoid biosynthesis and phytohormones. In addition, further studies suggest that MtHAN1 and MtHAN2 are required for the expression of several nodule-specific cysteine-rich genes, which they may activate directly, and many peptidase and peptidase inhibitor genes. This work expands our knowledge of the functions of MtHANs in plants by revealing an unexpected role in legume nodulation.

In this paper, by analyzing the limitations of the traditional trigonometric elevation method, and on the basis of establishing a mathematical model of trigonometric elevation, a convenient and fast trigonometric elevation method without instrument height and prism height is improved, and the operation steps of the method are introduced in detail. The accuracy analysis was evaluated and the method was applied to actual engineering. The results show that under the premise of ensuring the measurement accuracy, this method not only reduces the range of measurement error sources, but also improves the measurement efficiency.