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"栀子甙"
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Identification of Gardenoside and Sarsasapogenin from Qingwen Baidu Granules by TLC
by
Guo Shijin Wang Yanping Zhang Zhimei Fu shijun Xu Qianqian Zhang Ying Shen Zhiqiang
in
快速检测
,
硫酸溶液
,
薄层色谱鉴别
2016
[ Objective ] We aimed to establish the identification method of gardenoside and sarsasapogenin in Qingwen baidu granules. [ Method] With ethyl ace- tate-acetone-formic acid-water (10:7:2:0.5) as the developer and 10% sulfuric acid ethanol solution as the chromogenic reagent, the samples were heated at 100 ~C until the spots were clearly visible, and the existence of gardenoside was checked under natural light. With toluene-acetone(9:l ) as the developer and 5% vanillin sulfuric acid solution as the chromogenic reagent, the samples were heated at 105 ~C until the spots were clearly visible, and the existence of sarsasapogenin was checked under natural light. [Result] Qingwen baidu granules had the same spots with gardenoside and sarsasapogenin at the same Rfvalue under natural light. [ Conclusion] A TLC method detecting gardenoside and sarsasapogenin in Qingwen baidu granules was established, which had good specificity and repeatability, suitable for rapid detection of gardenoside and sarsasapogenin.
Journal Article