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Livestock dung is a valuable material for reconstructing human and animal inter-relations and activity within open areas and built environments. This paper examines the identification and multi-disciplinary analysis of dung remains from three neighbouring sites in the Konya Plain of Central Anatolia, Turkey: Boncuklu (ninth–eighth millennium cal BC), the Çatalhöyük East Mound (eighth–sixth millennium cal BC), and the Late Neolithic occupation at the Pınarbaşı rockshelter (seventh millennium cal BC). It presents and evaluates data on animal management strategies and husbandry practices through the simultaneous examination of plant and faecal microfossils and biomarkers with thin-section micromorphology and integrated phytolith, dung spherulite, and biomolecular analyses, together with comparative reference geo-ethnoarchaeological assemblages. Herbivore dung and other coprogenic materials have been identified predominantly in open areas, pens and midden deposits through micromorphology and the chemical signatures of their depositional contexts and composition. Accumulations of herbivore faecal material and burnt remains containing calcitic spherulites and phytoliths have provided new information on animal diet, fodder and dung fuel. Evidence from phytoliths from in situ penning deposits at early Neolithic Çatalhöyük have provided new insights into foddering/grazing practices by identifying highly variable herbivorous regimes including both dicotyledonous and grass-based diets. This review illustrates the variability of dung deposits within early agricultural settlements and their potential for tracing continuity and change in ecological diversity, herd management strategies and foddering, health, energy and dung use, as well as the complexity of interactions between people and animals in this key region during the early Holocene.

The early village at Çatalhöyük (7100–6150 BC) provides important evidence for the Neolithic and Chalcolithic people of central Anatolia. This article reports on the use of lipid biomarker analysis to identify human coprolites from midden deposits, and microscopy to analyse these coprolites and soil samples from human burials. Whipworm (Trichuris trichiura) eggs are identified in two coprolites, but the pelvic soil samples are negative for parasites. Çatalhöyük is one of the earliest Eurasian sites to undergo palaeoparasitological analysis to date. The results inform how intestinal parasitic infection changed as humans modified their subsistence strategies from hunting and gathering to settled farming.

Younger Dryas and early Holocene Western Stemmed Tradition occupants of the northern Great Basin appear to have practiced a broad-based subsistence strategy including the consumption of a wide variety of small animal and plant resources. However, much of our evidence for human diet and land use during this period comes from dry cave and rockshelter sites where it can be challenging to distinguish plant and small animal remains deposited as a result of human versus nonhuman activity. This study presents new direct evidence for Younger Dryas and early Holocene human diet in the northern Great Basin through multiproxy analysis of nine human coprolites from the Paisley Caves, Oregon, USA. The evidence indicates that Western Stemmed Tradition occupants consumed plants, small mammals, fish, and insects, including direct evidence for consumption of whole rodents and several types of beetle. Occupation of the caves occurred during the summer and fall by individuals foraging on wetland, sagebrush grassland, and riparian ecological landscapes suggesting geographical and seasonal variability in land-use patterns during the Younger Dryas and early Holocene periods. This research suggests that Western Stemmed Tradition settlement patterns were seasonally centered on productive valley bottom lakes and wetlands but also included forays to a variety of ecological landscapes. The results highlight the importance of plant and small animal resources in the human diet during the terminal Pleistocene settlement of North America and contribute to debates about the process of the peopling of the Americas.

In the field of archaeological parasitology, researchers have long documented the distribution of parasites in archaeological time and space through the analysis of coprolites and human remains. This area of research defined the origin and migration of parasites through presence/absence studies. By the end of the 20th century, the field of pathoecology had emerged as researchers developed an interest in the ancient ecology of parasite transmission. Supporting studies were conducted to establish the relationships between parasites and humans, including cultural, subsistence, and ecological reconstructions. Parasite prevalence data were collected to infer the impact of parasitism on human health. In the last few decades, a paleoepidemiological approach has emerged with a focus on applying statistical techniques for quantification. The application of egg per gram (EPG) quantification methods provide data about parasites’ prevalence in ancient populations and also identify the pathological potential that parasitism presented in different time periods and geographic places. Herein, we compare the methods used in several laboratories for reporting parasite prevalence and EPG quantification. We present newer quantification methods to explore patterns of parasite overdispersion among ancient people. These new methods will be able to produce more realistic measures of parasite infections among people of the past. These measures allow researchers to compare epidemiological patterns in both ancient and modern populations.

Phosphatic concretions in terrestrial settings are often identified as coprolites based upon their biotic contents and high phosphorus levels. However, recent discoveries have shown that non-fecal origins of fossiliferous phosphatic concretions are more common than originally recognized. Confusion about the taphonomic origin of phosphatic concretions can lead to erroneous paleobiological and paleoenvironmental interpretations, so a set of criteria would be useful to evaluate whether a phosphatic concretion is a coprolite. Here we describe a phosphatic concretion containing a small crocodylian from the Upper Cretaceous Hell Creek Formation and assess its origin, formation, and paleobiological implications. We conducted neutron computed tomography (CT) on the skull-bearing portion of the concretion, and analyzed the geochemical composition of the concretion with electron microprobe, µ-XRF, and fusion ICP-OES. In this study, the completeness and distribution of the skeletal elements present a stronger case for a non-fecal origin. This scenario suggests minimal transport after death and deposition. Neutron CT analysis of the crocodylian skull supports its referral to Brachychampsa montana , and allows inferences regarding body length, age, and dietary habits. Although coprolites and non-fecal concretions can be difficult to differentiate, unique features can reflect differences in origin that offer different types of taphonomic and paleobiological information.

The impact of coprolite taphonomy on parasite remains and aDNA recovery has been recognized. In general, coprolites from sites protected by geologic features such as caves and rock shelters exhibit the best preservation. In contrast, coprolites from open sites can be badly affected by taphonomic processes as shown by analyses of parasite eggs. For eggs, the impact of mites and free-living nematodes has been quantified. Mites are associated with poor pinworm egg preservation. In other studies, percolation of water through sediments has a negative impact on egg recovery. We note that dietary remains can also decompose at open sites. Through scanning electron microscopy (SEM), we present examples of screened but chemically untreated microscopic remains. “Panorama” SEM images provide an excellent visual overview of the taphonomy of dietary remains. For this study, our focus is on Southwestern coprolites as a demonstration of diversity within a single region. Examples from caves and rock shelters were examined first to describe the taphonomic challenges for protected sites. Then, attention was turned to coprolites from open sites. In general, the challenges noted for parasite preservation are seen for other microfossils. However, the preservation of lignin, sporopollenin, calcium oxalate, and siliceous microfossils is generally better than cellulose structures. These observations are relevant to the selection process of samples for aDNA analysis and immunological study. This is especially relevant for the gut microbiome since decomposer fungi and bacteria molecular signals could be recovered in metagenomic analysis.

Human microbiome studies are increasingly incorporating macroecological approaches, such as community assembly, network analysis and functional redundancy to more fully characterize the microbiome. Such analyses have not been applied to ancient human microbiomes, preventing insights into human microbiome evolution. We address this issue by analysing published ancient microbiome datasets: coprolites from Rio Zape ( n = 7; 700 CE Mexico) and historic dental calculus ( n = 44; 1770–1855 CE, UK), as well as two novel dental calculus datasets: Maya ( n = 7; 170 BCE-885 CE, Belize) and Nuragic Sardinians ( n = 11; 1400–850 BCE, Italy). Periodontitis-associated bacteria ( Treponema denticola , Fusobacterium nucleatum and Eubacterium saphenum ) were identified as keystone taxa in the dental calculus datasets. Coprolite keystone taxa included known short-chain fatty acid producers ( Eubacterium biforme, Phascolarctobacterium succinatutens ) and potentially disease-associated bacteria ( Escherichia , Brachyspira) . Overlap in ecological profiles between ancient and modern microbiomes was indicated by similarity in functional response diversity profiles between contemporary hunter–gatherers and ancient coprolites, as well as parallels between ancient Maya, historic UK, and modern Spanish dental calculus; however, the ancient Nuragic dental calculus shows a distinct ecological structure. We detected key ecological signatures from ancient microbiome data, paving the way to expand understanding of human microbiome evolution. This article is part of the theme issue ‘Insights into health and disease from ancient biomolecules’.

The Paisley Cave archeological site in the Northern Great Basin has provided a rich archaeological record from 13,000 to 6000 cal yr BP, including abundant mammalian coprolites preserved in a well-dated stratigraphy. Here we analyze and contrast pollen from within coprolites and pollen in associated sediments to examine vegetation history and assess whether coprolite pollen provides unique information with respect to the coprolite producer, such as the use of specific habitats, foods, or water sources. We found that the dissimilarity of pollen assemblages between coprolites and associated sediments was greater than the serial dissimilarity between stratigraphically adjacent samples within either group. Serial dissimilarity within types was not greater for coprolites than sediments, as would be expected if there were unique pollen signatures derived from the short period (1–2 days) represented by each coprolite. Compared with sediment pollen assemblages, the coprolites had higher abundances of lighter pollen types, and some individual samples were high in wetland taxa (especially Typha). Our results are consistent with coprolite pollen representing short time periods collected as a mammal moves on the landscape, whereas sediment pollen reflects longer time periods and more regional vegetation indicators.

Archaeological dung pellets are time capsules of ancient herbivore diets and gut flora, informing on past agropastoral activity, ecology, and animal health. Improving multi-proxy approaches is key to maximizing this finite archaeological resource. Through experiments with standard pretreatments used in radiocarbon (14C) dating, we address a fundamental problem in maximal multi-proxy analysis: How to chronometrically date individual caprine pellets while conserving as much as possible for additional analyses? We applied acid-alkali-acid (AAA) or acid-only pretreatments to 37 samples of ancient and recent sheep/goat dung pellets from sites in the Negev desert, Israel, measuring weight-loss due to pretreatment. Shavings of outer surfaces and remaining inner pellets of four pairs were dated and compared. We found that (i) sample-specific factors affect pretreatment survivability, including preservation quality and initial sample size; (ii) given sufficient start weight, AAA can be used to pretreat sheep/goat coprolites; (iii) 100 mg appeared a desirable minimum sample weight before pretreatment; and (iv) shavings of coprolites’ outer surface produced 14C dates equivalent to dates obtained from inner coprolites. Whereas standard coprolite analysis protocols discard shavings removed from outer surfaces to avoid contamination, our findings indicate their efficacy for 14C dating. This offers an important addition to workflows for multi-proxy coprolite analysis.

Coprolites, ., fossilized faeces, are an important source of knowledge on the diet and food processing mechanisms in the fossil record. Direct and indirect evidences for the dietary preferences of extinct sharks are rare in the fossil record. The first coprolite attributable to containing prey remains from the European Cretaceous is documented here. A coprolite from the Late Cretaceous of Opole (southern Poland) was scanned using micro-computed tomography to show the arrangement of the inclusions. In addition, the cross-section was examined under the SEM/EDS to analyse the microstructure and chemical composition of the inclusions. Brachiopod shell fragments and foraminiferan shells are recognized and identified among the variously shaped inclusions detected through the performed analysis. The extinct shell-crushing shark has been identified as the likely producer of the examined coprolite. The presence of brachiopod shell fragments indicates that at least some species of this durophagous predatory shark may have preyed on small benthic elements on the sea bottom.