Explore the vast range of titles available.

In 1900, some 100,000 people living in Bulgaria-2 percent of the country's population-could be described as Greek, whether by nationality, language, or religion. The complex identities of the population-proud heirs of ancient Hellenic colonists, loyal citizens of their Bulgarian homeland, members of a wider Greek diasporic community, devout followers of the Orthodox Patriarchate in Istanbul, and reluctant supporters of the Greek government in Athens-became entangled in the growing national tensions between Bulgaria and Greece during the first half of the twentieth century. InBetween Two Motherlands, Theodora Dragostinova explores the shifting allegiances of this Greek minority in Bulgaria. Diverse social groups contested the meaning of the nation, shaping and reshaping what it meant to be Greek and Bulgarian during the slow and painful transition from empire to nation-states in the Balkans. In these decades, the region was racked by a series of upheavals (the Balkan Wars, World War I, interwar population exchanges, World War II, and Communist revolutions). The Bulgarian Greeks were caught between the competing agendas of two states increasingly bent on establishing national homogeneity. Based on extensive research in the archives of Bulgaria and Greece, as well as fieldwork in the two countries, Dragostinova shows that the Greek population did not blindly follow Greek nationalist leaders but was torn between identification with the land of their birth and loyalty to the Greek cause. Many emigrated to Greece in response to nationalist pressures; others sought to maintain their Greek identity and traditions within Bulgaria; some even switched sides when it suited their personal interests. National loyalties remained fluid despite state efforts to fix ethnic and political borders by such means as population movements, minority treaties, and stringent citizenship rules. The lessons of a case such as this continue to reverberate wherever and whenever states try to adjust national borders in regions long inhabited by mixed populations.

Sex estimation is one of the primary steps for constructing the biological profile of skeletal remains leading to their identification in the forensic context. While the pelvis is the most sex diagnostic bone, the cranium and other post-cranial elements have been extensively studied. Earlier research has also focused on the vertebral column with varying results regarding its sex classification accuracy as well as the underlying population specificity. The present study focuses on three easily identifiable vertebrae, namely T1, T12, and L1, and utilizes two modern European populations, a Greek and a Danish, to evaluate their forensic utility in sex identification. To this end, 865 vertebrae from 339 individuals have been analyzed for sexual dimorphism by further evaluating the effects of age-at-death and population affinity on its expression. Our results show that T1 is the best sex diagnostic vertebra for both populations reaching cross-validated accuracy of almost 90%, while age-at-death has limited effect on its sexual dimorphism. On the contrary, T12 and L1 produced varying results ranging from 75 to 83% accuracy with the Greek population exhibiting distinctively more pronounced sexual dimorphism. Additionally, age-at-death had significant effect on sexual dimorphism of T12 and L1 and especially in the Greek female and Danish male groups. Our results on inter-population comparison suggest that vertebral sex discriminant functions, and especially those utilizing multiple measurements, are highly population specific and optimally suitable only for their targeted population. An open-source software tool to facilitate classifying new cases based on our results is made freely available to forensic researchers.

Background Expression quantitative trait loci (eQTL) studies provide insights into regulatory mechanisms underlying disease risk. Expanding studies of gene regulation to underexplored populations and to medically relevant tissues offers potential to reveal yet unknown regulatory variants and to better understand disease mechanisms. Here, we performed eQTL mapping in subcutaneous (S) and visceral (V) adipose tissue from 106 Greek individuals (Greek Metabolic study, GM) and compared our findings to those from the Genotype-Tissue Expression (GTEx) resource. Results We identified 1,930 and 1,515 eGenes in S and V respectively, over 13% of which are not observed in GTEx adipose tissue, and that do not arise due to different ancestry. We report additional context-specific regulatory effects in genes of clinical interest (e.g. oncogene ST7 ) and in genes regulating responses to environmental stimuli (e.g. MIR21, SNX33 ). We suggest that a fraction of the reported differences across populations is due to environmental effects on gene expression, driving context-specific eQTLs, and suggest that environmental effects can determine the penetrance of disease variants thus shaping disease risk. We report that over half of GM eQTLs colocalize with GWAS SNPs and of these colocalizations 41% are not detected in GTEx. We also highlight the clinical relevance of S adipose tissue by revealing that inflammatory processes are upregulated in individuals with obesity, not only in V, but also in S tissue. Conclusions By focusing on an understudied population, our results provide further candidate genes for investigation regarding their role in adipose tissue biology and their contribution to disease risk and pathogenesis.

We investigated whether disturbance of glycocalyx integrity is related with increased cardiovascular risk. In 600 healthy subjects, we measured perfused boundary region (PBR), a marker of glycocalyx integrity, in sublingual microvessels with diameter ranging 5–25 µm using a dedicated camera (Sideview Darkfield Imaging). Increased PBR indicates reduced glycocalyx thickness. We prospectively monitored the occurrence of cardiovascular events (MACE-death, myocardial infarction, and stroke) during a 6-year follow-up. Fifty-seven MACE were documented. Increased values of PBR5-25 predicted higher risk for MACE in a model including sex, age, hyperlipidemia, diabetes, hypertension, smoking, family history of coronary disease, treatment with ACEi/ARBs, or lipid-lowering agents (hazard ratio (HR), 6.44, p = 0.011; net reclassification improvement (NRI), 28%; C-statistic: 0.761). PBR5-25 was an independent and additive predictor of outcome when added in a model including the European Heart SCORE, diabetes, family history of CAD, and medication (HR, 4.71; NRI: 39.7%, C-statistic from 0.653 to 0.693; p < 0.01).Glycocalyx integrity is an independent and additive predictor to risk factors for MACE at 6-year follow-up in individuals without cardiovascular disease. ClinicalTrials.govIdentifier:NCT04646252.Graphical abstractPBR5-25 was an independent and additive predictor of adverse cardiovascular events in a model including the European Heart SCORE, diabetes, family history of coronary disease, and medication (HR: 4.71, NRI: 39.7%, C-statistic from 0.653 to 0.693; p < 0.01, NRI:37.9%).

Tacrolimus, an approved first-line calcineurin inhibitor, is widely prescribed in organ transplantation to prevent allograft rejection. Its narrow therapeutic index requires precise management to achieve optimal dosing and to minimize adverse drug events (ADEs) while ensuring its therapeutic efficacy. Among several factors, genetic differences contribute significantly to the inter-individual and inter-ethnic variability in pharmacokinetics (PK) of tacrolimus in kidney transplant recipients. As a result, investigating the role of genetic variation in Greek transplant recipients becomes crucial to optimizing therapeutic strategies and enhancing the efficacy of immunosuppressive treatment. Genetic variants which are known to influence the activity of enzymes or drug-transporters critical to tacrolimus pharmacokinetics, may significantly affect the required kidney post-transplant tacrolimus daily dose. To assess the correlation of genetic variants (rs1128503, rs2229109) and (rs2242480, rs4986910) with tacrolimus dose-adjusted trough concentration (C /D), in Greek kidney transplant recipients. Ninety-four unrelated Greek kidney transplant recipients were included in this study from the Department of Nephrology and Kidney Transplantation of the University General Hospital of Patras. Patients' dose-adjusted trough levels were measured at five distinct time points after transplantation and analyzed in relation to the possible influence of and correlated with the abovementioned genetic variants using standard genotyping analysis and Sanger sequencing. The genetic variants rs1128503, rs2229109, rs2242480, rs4986910 did not show any significant association with the daily dosing requirements of tacrolimus for at least 1 year, in Greek patients who have undergone kidney transplant. It remains uncertain whether these genetic variants influence the assessment of the appropriate tacrolimus dosing 1 year after transplantation in Greek kidney transplant recipients.

Background: Association studies of vitamin D receptor (VDR) polymorphisms with COVID-19 severity have produced inconsistent results in different populations. Herein we examined VDR gene polymorphisms in a Caucasian Greek cohort of COVID-19 patients. Methods: This was a case-control study in a tertiary university hospital in Greece including 137 COVID-19 patients with varying disease severities and 72 healthy individuals. In total 209 individuals were genotyped for the FokI (rs10735810), ApaI (rs7975232), TaqI (rs731236) and BsmI (rs1544410) single-nucleotide polymorphisms (SNP) of the VDR gene by polymerase chain reaction and restriction fragment length polymorphism analysis (PCR-RFLPs). Statistical analyses were performed to determine the association between genotype and disease severity, adjusting for various confounding factors. Results: Genotype distribution of the studied VDR SNPs in the control group was in Hardy–Weinberg equilibrium. The TaqI variant was differentially distributed between controls and COVID-19 patients according to the additive model (p = 0.009), and the CC genotype was significantly associated with an increased risk for severe COVID-19 according to the recessive model [OR: 2.52, 95%CI:1.2–5.29, p = 0.01]. Multivariate analysis demonstrated a robust association of COVID-19 severity and TaqI polymorphism in the recessive model even after adjusting for multiple confounders, including age, sex and CRP levels [Adj.OR:3.23, 95%CI:1.17–8.86, p = 0.023]. The distribution of FokI, ApaI and BsmI genotypes was similar between COVID-19 patients and controls. Conclusions: The CC genotype of TaqI polymorphism is significantly associated with an increased risk for severe COVID-19 independently of age, sex or degree of inflammation.

Background/Objectives: The mental foramen (MF) constitutes a passage for mental nerves and vessels, and it is a crucial anatomical landmark in the body of the mandible. The accessory mental foramen (AMF) is a small, addable foramen proximate to the MF, and it is mainly located posteriorly. The AMF is a rare anatomical variation in human mandibles that must be taken into consideration throughout dental and surgical operations. We aimed to assess the incidence and perform morphological and morphometric analyses of AMFs in the human dry mandibles of the Greek population, in addition to a relevant systematic review and meta-analysis of global data. Methods: We studied 114 human adult dry mandibles of unknown gender and age available from the Laboratory of Anatomy, Medical School, Democritus University of Thrace, Greece. We used the search term “accessory mental foramen” in the PubMed, Scopus, and Google Scholar databases to detect all publications of the last 50 years reporting the prevalence and morphology of AMFs in dry mandibles; the search ended on 13 January 2025. Quality assessments were performed using the relevant Joanna Briggs Institute tool. Data were synthesized with the random-effects REML model after Freeman–Tukey double arcsine transformationusing STATA 18. No external funding was received. The PROSPERO CRD is 42025638135. Results: According to our data, the MF was present in all observed mandibles, and it was bilateral. Nine AMFs (five right/four left; five round/four oval; six posterior/three anterior to the MF) were found in seven mandibles (five single and two double), and all were unilateral. AMFs presented a mean diameter of 0.96 ± 0.43 mm and mean distances of 4.12 ± 2.15 mm from the MF, 12.68 ± 4.10 mm from the alveolar ridge, and 11.92 ± 1.57 mm from the lower border of the mandible. Furthermore, 27 publications were included in the meta-analysis; the combined AMF prevalence was 6.1% (95% CI: 4.8–7.6%; I2 60%), the combined mean vertical axis was 1.18 ± 0.61 mm, and the combined mean distance from the MF was 3.64 ± 2.29 mm. Bilateral AMFs were detected in 2.1% of AMF cases. An oval shape was described in 37.3% of AMFs. No publication bias was detected. Conclusions: AMFs are not considered rare, and they are occasionally bilateral or even multiple in number. Moreover, they demonstrate considerable variation regarding their size, shape, anddistance from the MF, alveolar ridge, and lower border of the mandible. Dental surgeons must be aware of AMFs’ anatomical variations during surgical and anesthetic planning in order to effectively prevent or mitigate the risk of postoperative complications, such as pain, anesthesia, injury, and other adverse outcomes.

•Testing 158 crania of adult individuals from the Athens Collection.•3D-ID exhibits moderate classification performance.•The use of 19-landmark configuration improves classification accuracy of ancestry.•The classification accuracy of sex increases when form variables are used.•3D-ID could be utilized to successfully discriminate major ancestral groups. A primary concern in forensic anthropology, when reconstructing the biological profile of an unidentified individual is ancestry and sex estimation. The development of multivariate statistical methods and the assembly of large reference sample databases gave rise to the development of specialized computer software for sex and ancestry estimation. Among various such software, the 3D-ID is the only freely-available program that can handle missing values in the input dataset. The present study evaluates the reliability of 3D-ID in correctly classifying ancestry and sex of 158 test subjects from the Athens Collection, a documented Greek population sample. 3D-ID’s classification performance was evaluated both separately and collectively for sex and ancestry. According to our results, the accuracy regarding sex estimation ranged from 74.05% to 86.7% for cases with unknown ancestry and reached 89.87% when testing within the Southeastern European reference group, whereas ancestry estimation accuracy reached 70.9% for correctly classifying the Greek individuals to European population groups. We conclude that 3D-ID software exhibits moderate reliability in ancestry estimation and adequate reliability in sex estimation. The Greek population seems to deviate from the 3D-ID software's reference samples and therefore caution should be taken in interpreting 3D-ID’s results of unknown subjects, for which the software's reference sample database may not be representative. 3D-ID’s guidelines for using 19-landmark configuration improves the accuracy of ancestry estimation and form variables should be preferred for sexing samples.

Certain microRNAs (miRs) present in human plasma are candidate biomarkers for cardiovascular diseases, including acute myocardial infarction (AMI). We examined the expression of two cardiac-specific miRs (miR-208b and miR-499) in a Greek pathological population. Plasma samples from AMI patients and healthy subjects (controls) were analyzed using TaqMan® MicroRNA assays. The concentration of both miRs was significantly elevated in AMI patients compared to healthy controls. Moreover, receiver-operating characteristic (ROC) curve analysis showed that miR-208b and miR-499 displayed similar properties with the established AMI biomarker cardiac troponin T (cTnT). We showed, for the first time, that these miRs could be used as AMI biomarkers in our population as well. Our data are in agreement with those of studies based on different population groups and further strengthen the observation that plasma levels of circulating miR-208b and miR-499 could serve as potential AMI biomarkers.

This paper introduces an automated method for estimating sex from the lower and upper limbs based on diaphyseal CSG properties. The proposed method was developed and evaluated using 389 femurs, 412 tibias, and 404 humeri of adult individuals from a modern Greek reference sample, the Athens Collection. The skeletal properties, which were extracted with the CSG-Toolkit, were analyzed with step-wise DFA (evaluated with LOOCV) and subsequently with RBF kernel SVM supervised learning. SVM cross-validation was based on a 20-fold stratified random sample splitting as well as a chronological split based on year of birth to further assess the effect of secular change in sex estimation capacity. Maximum cross-validated classification accuracy from step-wise DFA reached 94.8% for the femur, 94.7% for the tibia, and 97.3% for the humerus, whereas SVM cross-validated results were similar although slightly lower, mainly due to the more strict cross-validation scheme. Our results suggest that the proposed sex estimation method is reasonably robust to secular change, since there was limited loss in classification accuracy between different chronological groups, despite the presence of secular change in stature of the Greek population during the examined period. The proposed method has been implemented as a function for the GNU Octave environment, named estimate_sex, which comprises a self-intuitive graphical user interface for facilitating sex estimation and is freely available under a suitable license.