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"Plant genetic engineering."
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Seeds, science, and struggle
Genetic engineering has a wide range of cultural, economic, and ethical implications, yet it has become almost an article of faith that regulatory decisions about biotechnology be based only on evidence of specific quantifiable risks; to consider anything else is said to \"politicize\" regulation. In this study of social protest against genetically engineered food, Abby Kinchy turns the conventional argument on its head. Rather than consider politicization of the regulatory system, she takes a close look at the scientization of public debate about the \"contamination\" of crops resulting from pollen drift and seed mixing. Advocates of alternative agriculture confront the scientization of this debate by calling on international experts, carrying out their own research, questioning regulatory science in court, building alternative markets, and demanding that their governments consider the social and economic impacts of the new technologies. Kinchy focuses on social conflicts over canola in Canada and maize in Mexico, drawing out their linkages to the global food system and international environmental governance. The book ultimately demonstrates the shortcomings of dominant models of scientific risk governance, which marginalize alternative visions of rural livelihoods and sustainable food production.The hardcover edition does not include a dust jacket.
eBook
Evolution Made to Order
Plant breeders have long sought technologies to extend human control over nature. Early in the twentieth century, this led some to experiment with startlingly strange tools like x-ray machines, chromosome-altering chemicals, and radioactive elements. Contemporary reports celebrated these mutation-inducing methods as ways of generating variation in plants on demand. Speeding up evolution, they imagined, would allow breeders to genetically engineer crops and flowers to order. Creating a new food crop or garden flower would soon be as straightforward as innovating any other modern industrial product.
In Evolution Made to Order, Helen Anne Curry traces the history of America's pursuit of tools that could intervene in evolution. An immersive journey through the scientific and social worlds of midcentury genetics and plant breeding and a compelling exploration of American cultures of innovation, Evolution Made to Order provides vital historical context for current worldwide ethical and policy debates over genetic engineering.
eBook
GMOs
An overview of genetic modification and how this biotechnology affects food, humans, animals, and medicine. Includes a discussion of the ethical concerns of genetic biotechnology.
Book
Seeds, science, and struggle : the global politics of transgenic crops / Abby Kinchy
An examination of how advocates for alternative agriculture confront \"science-based\" regulation of genetically engineered crops.
eBook
The complex architecture and epigenomic impact of plant T-DNA insertions
The bacterium Agrobacterium tumefaciens has been the workhorse in plant genome engineering. Customized replacement of native tumor-inducing (Ti) plasmid elements enabled insertion of a sequence of interest called Transfer-DNA (T-DNA) into any plant genome. Although these transfer mechanisms are well understood, detailed understanding of structure and epigenomic status of insertion events was limited by current technologies. Here we applied two single-molecule technologies and analyzed Arabidopsis thaliana lines from three widely used T-DNA insertion collections (SALK, SAIL and WISC). Optical maps for four randomly selected T-DNA lines revealed between one and seven insertions/rearrangements, and the length of individual insertions from 27 to 236 kilobases. De novo nanopore sequencing-based assemblies for two segregating lines partially resolved T-DNA structures and revealed multiple translocations and exchange of chromosome arm ends. For the current TAIR10 reference genome, nanopore contigs corrected 83% of non-centromeric misassemblies. The unprecedented contiguous nucleotide-level resolution enabled an in-depth study of the epigenome at T-DNA insertion sites. SALK_059379 line T-DNA insertions were enriched for 24nt small interfering RNAs (siRNA) and dense cytosine DNA methylation, resulting in transgene silencing via the RNA-directed DNA methylation pathway. In contrast, SAIL_232 line T-DNA insertions are predominantly targeted by 21/22nt siRNAs, with DNA methylation and silencing limited to a reporter, but not the resistance gene. Additionally, we profiled the H3K4me3, H3K27me3 and H2A.Z chromatin environments around T-DNA insertions using ChIP-seq in SALK_059379, SAIL_232 and five additional T-DNA lines. We discovered various effect s ranging from complete loss of chromatin marks to the de novo incorporation of H2A.Z and trimethylation of H3K4 and H3K27 around the T-DNA integration sites. This study provides new insights into the structural impact of inserting foreign fragments into plant genomes and demonstrates the utility of state-of-the-art long-range sequencing technologies to rapidly identify unanticipated genomic changes.
Journal Article
Targeted base editing in rice and tomato using a CRISPR-Cas9 cytidine deaminase fusion
Targeted editing of single base pairs is achieved in monocot rice and dicot tomato using Target-AID (Cas9 activation-induced cytidine deaminase fusion).
We applied a fusion of CRISPR-Cas9 and activation-induced cytidine deaminase (Target-AID) for point mutagenesis at genomic regions specified by single guide RNAs (sgRNAs) in two crop plants. In rice, we induced multiple herbicide-resistance point mutations by multiplexed editing using herbicide selection, while in tomato we generated marker-free plants with homozygous heritable DNA substitutions, demonstrating the feasibility of base editing for crop improvement.
Journal Article