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Targeted base editing in rice and tomato using a CRISPR-Cas9 cytidine deaminase fusion

by Miura, Kenji , Kondo, Akihiko , Ishii, Hisaki , Komatsu, Hiroki , Kashojiya, Sachiko , Yamamoto, Tsuyoshi , Shimatani, Zenpei , Takayama, Mariko , Teramura, Hiroshi , Nishida, Keiji , Ariizumi, Tohru , Ezura, Hiroshi , Arazoe, Takayuki , Terada, Rie

in 45/41 / 45/70 / 631/449/711 / 631/553/2690 / Activation-induced cytidine deaminase / Agriculture / Base Pairing - genetics / Bioinformatics / Biomedical and Life Sciences / Biomedical Engineering/Biotechnology / Biomedicine / Biotechnology / brief-communication / Cereal crops / Cloning / Clustered Regularly Interspaced Short Palindromic Repeats - genetics / CRISPR / CRISPR-Associated Proteins - genetics / Crop improvement / Crops / Cytidine deaminase / Cytidine Deaminase - genetics / Deoxyribonucleic acid / DNA / DNA, Plant - genetics / Editing / Enzymes / Gene Editing - methods / Genes / Genes, Plant - genetics / Genetic aspects / Genetic engineering / Herbicide resistance / Herbicides / Life Sciences / Lycopersicon esculentum / Lycopersicon esculentum - genetics / Methods / Mutagenesis / Mutagenesis, Site-Directed - methods / Mutation / Oryza - genetics / Plant genetic engineering / Plants, Genetically Modified - genetics / Point mutation / Point Mutation - genetics / Recombinant Fusion Proteins - genetics / Ribonucleic acid / Rice / RNA / Tomatoes

2017

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Targeted base editing in rice and tomato using a CRISPR-Cas9 cytidine deaminase fusion

2017

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Targeted base editing in rice and tomato using a CRISPR-Cas9 cytidine deaminase fusion

2017

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Journal Article

Targeted base editing in rice and tomato using a CRISPR-Cas9 cytidine deaminase fusion

Miura, Kenji,

Kondo, Akihiko,

Ishii, Hisaki,

Komatsu, Hiroki,

Kashojiya, Sachiko,

Yamamoto, Tsuyoshi,

Shimatani, Zenpei,

Takayama, Mariko,

Teramura, Hiroshi,

Nishida, Keiji,

Ariizumi, Tohru,

Ezura, Hiroshi,

Arazoe, Takayuki,

Terada, Rie

2017

Overview

Targeted editing of single base pairs is achieved in monocot rice and dicot tomato using Target-AID (Cas9 activation-induced cytidine deaminase fusion). We applied a fusion of CRISPR-Cas9 and activation-induced cytidine deaminase (Target-AID) for point mutagenesis at genomic regions specified by single guide RNAs (sgRNAs) in two crop plants. In rice, we induced multiple herbicide-resistance point mutations by multiplexed editing using herbicide selection, while in tomato we generated marker-free plants with homozygous heritable DNA substitutions, demonstrating the feasibility of base editing for crop improvement.

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Publisher

Springer New York,Nature Publishing Group

Subject

45/41

/ 45/70

/ 631/449/711

/ 631/553/2690

/ Activation-induced cytidine deaminase

/ Agriculture

/ Base Pairing - genetics