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The most common and cheap indirect technique to measure relative humidity is by using psychrometer based on a dry and a wet temperature sensor. In this study, the measurement uncertainty of relative humidity was evaluated by this indirect method with some empirical equations for calculating relative humidity. Among the six equations tested, the Penman equation had the best predictive ability for the dry bulb temperature range of 15–50 °C. At a fixed dry bulb temperature, an increase in the wet bulb depression increased the error. A new equation for the psychrometer constant was established by regression analysis. This equation can be computed by using a calculator. The average predictive error of relative humidity was <0.1% by this new equation. The measurement uncertainty of the relative humidity affected by the accuracy of dry and wet bulb temperature and the numeric values of measurement uncertainty were evaluated for various conditions. The uncertainty of wet bulb temperature was the main factor on the RH measurement uncertainty.

The classical view that the drought-related hormone ABA simply acts locally at the guard cell level to induce stomatal closure is questioned by differences between isolated epidermis and intact leaves in stomatal response to several stimuli. We tested the hypothesis that ABA mediates, in addition to a local effect, a remote effect in planta by changing hydraulic regulation in the leaf upstream of the stomata. By gravimetry, porometry to water vapour and argon, and psychrometry, we investigated the effect of exogenous ABA on transpiration, stomatal conductance and leaf hydraulic conductance of mutants described as ABA-insensitive at the guard cell level. We show that foliar transpiration of several ABA-insensitive mutants decreases in response to ABA. We demonstrate that ABA decreases stomatal conductance and down-regulates leaf hydraulic conductance in both the wildtype Col-0 and the ABA-insensitive mutant ost2-2. We propose that ABA promotes stomatal closure in a dual way via its already known biochemical effect on guard cells and a novel, indirect hydraulic effect through a decrease in water permeability within leaf vascular tissues. Variability in sensitivity of leaf hydraulic conductance to ABA among species could provide a physiological basis to the isohydric or anisohydric behaviour.

Animal welfare and productive performance are compromised when animals are housed in environments which place them outside their thermal comfort zone. However, the identification of thermal stress, when based on air properties, suggests the use of outdated and generic indices. The objective of this work was to develop and validate a methodology for classifying and diagnosing heat stress in production animals based on psychrometric air relations. The model was created for broilers, pigs, dairy cattle, and laying birds, categorized into a total of 21 breeding phases. For each phase, a bibliographic search was carried out for the psychrometric parameters of the air—dry bulb temperature (AT) and relative humidity (RH)—that satisfied the animals’ critical and ideal thermoneutral zones. Adding the local atmospheric pressure (AP), the parameters were used to calculate the enthalpy (h), resulting in five comfort ranges. Based on this, a decision tree was elaborated, consisting of three attributes (AT, RH, and h) and seven diagnostic classes, based on the psychrometric principles of air. The proposed methodology was used in a case study, with a database extracted from an individual shelter for calves. For the evaluation of the decision tree, two induction algorithms, ID3 and c4.5, were compared, both of which presented high accuracy and proposed simpler tree models than the one theoretically developed for the methodology. In conclusion, the methodology represents a great potential to characterize the thermal comfort of the animals, diagnose the causes of stress, and recommend possible corrective actions. The study revealed that decision trees can be adapted and simplified for each creation phase.

In this paper we describe a non-invasive method of measuring leaf water content using THz radiation and combine this with psychrometry for determination of leaf pressure–volume relationships. In contrast to prior investigations using THz radiation to measure plant water status, the reported method exploits the differential absorption characteristic of THz radiation at multiple frequencies within plant leaves to determine absolute water content in real-time. By combining the THz system with a psychrometer, pressure–volume curves were generated in a completely automated fashion for the determination of leaf tissue water relations parameters including water potential at turgor loss, osmotic potential at full turgor and the relative water content at the turgor loss point. This novel methodology provides for repeated, non-destructive measurement of leaf water content and greatly increased efficiency in generation of leaf PV curves by reducing user handling time.

Expansive soils, or reactive soils, experience moisture changes under the prevailing climate conditions, leading to shrink during dry periods and swell in wet months. Houses founded on such problematic soils are subjected to considerable ground surface movements, which can greatly affect the function and performance of buildings. Such movements will increase with the presence of a tree or tree groups nearby since tree roots can extract considerable amounts of water from soils, resulting in localized shrinkage settlement. In this study, a field site was selected with a large native spotted gum tree in a well-established eastern suburb of Melbourne, Victoria. The site and tree are fully instrumented. Surface and sub-surface movement pads, in situ soil psychrometers and neutron moisture meter access holes were installed to evaluate soil moisture/suction changes and subsequent ground movements. A sap flow meter that incorporated the heat ratio method was installed on the north side of the main trunk of the tree at breast height to enable sap velocity and sap flow volume to be closely monitored. Soil types, degree of reactivity and capability of soil water retention have been evaluated through a number of laboratory soil tests including shrink-swells, Atterberg limits, and soil water characteristic curve determination. This paper presents the preliminary monitoring results for a period of 6 months. Valuable field data such as this can help geotechnical engineers and practitioners to get a better understanding of the physical processes that drive tree root-expansive soil interaction and in time improve the current footing design guidelines.

The investigation of a solar collector is based on the thermal behaviour of a carrier fluid and the degradation of energy across a flat plate collector. The exergy analysis of a thermal system includes the change in the exergy function of a carrier fluid while transferring solar radiation across an air gap. The cell cast acrylic glass was used to transmit the incident solar radiation to the absorber plate, and to safeguard the absorber plate from the outside environment. With the help of the steady flow energy equation, the enthalpy of the carrier fluid (moist air) was calculated. The specific humidity of the incoming air was calculated at an average dry bulb temperature of 299.4 K. The stagnation temperature at a limiting condition was also estimated to find out the maximum permissible limit for a given thermal design. The mass flow rate of air was assumed to be 5.2 g-s −1 . The efficiency of the solar collector was found to vary from 40 to 42%, whereas the thermal energy available for drying was 15–59% of the exergy of the carrier fluid. The net entropy generation rate due to the collector plate was calculated to be 0.12 W-K −1 .

Regulation of tissue water potential is a key mechanism in macroalgal osmotic responses to changing external osmotic conditions, which are common in tidally influenced estuarine and intertidal systems. Nevertheless, significant knowledge gaps exist in our understanding of osmotic responses in macroalgae because few methods measure osmotic potential within macroalgal tissues. Leaf psychrometers have furthered understanding of osmotic potentials in terrestrial plant water relations, yet these have not been developed to measure the range of highly negative potential values found in marine macroalgae. To address these gaps, we present an effective, updated version of the Chardakov method to measure tissue water potential in macroalgae. Here, we present a case study examining macroalgal response in tissue water potential by two morphologically and evolutionarily distinct species, Ulva lactuca (Chlorophyta) and Hypnea musciformis (Rhodophyta) to four paired salinity and nutrient treatments at two temperatures. These treatments simulate a gradient from full coastal ocean conditions to brackish submarine groundwater discharge, an ecosystem type found on basaltic shorelines. Both algae demonstrated plasticity in osmotic response to submarine groundwater discharge with significant positive correlations between tissue water potential and proportion of submarine groundwater discharge in the treatment. These results are the first to describe macroalgal response in tissue water potential, a first step to understanding algal physiological ecology in such complex coastal environments. This revised Chardakov method is a valuable tool to better understand species-specific osmotic responses to ecologically relevant conditions, and can augment the study of other tidal systems and ontogenetic stages. Marine plants occurring in tidally-influenced estuarine nearshore ecosystems experience daily oscillations from fresh to marine salinities. Under the stress of changing external salinities regulation of solute concentrations within plant cells is critical to continued growth and photosynthesis. However, a lack of available methodologies has prevented significant breakthroughs in understanding of water relations in marine plants since the 1980s. This work updates the Chardakov Method, a classic method of measuring tissue water potential. This simple and cost-effective method is applied to examining the regulation of internal cellular solute concentrations for two Hawaiian marine plant species under simulated conditions of nearshore lowered salinity and higher nutrients. This work demonstrates the use of this methodology for measuring marine plant water potentials, and measures salinity-driven regulation of internal cellular solute concentrations for two species. The authors encourage the use of this method for further investigation of water relations in marine plants under oscillating salinities.

Wetwood, water accumulation in the heartwood of tree trunks, is a defect of forest trees and needs to be improved for wood utilization. To understand the mechanism of wetwood formation, differences in water potential between sapwood and heartwood and their seasonal changes were investigated. The water potential of specimens sampled from tree trunks using an increment corer was measured by psychrometry at four-week intervals for 2 years in two coniferous species. A water potential gradient from sapwood to heartwood was identified that could provide a driving force of the radial water movement, and this gradient was found to fluctuate seasonally. The two species studied differed in seasonal change in water potential. The source of the water potential gradient is thought to be heartwood-accumulated potassium and water-soluble heartwood substance. The hypothesis is proposed that water movement from sapwood to heartwood during wetwood formation involves vaporous rather than liquid water and occurs over a limited season.

Olive trees have a unique reproductive pattern marked by biennial fruiting. This study examined the repercussions of alternate fruit bearing on the water relations of olive trees and the associated ecophysiological mechanisms. The experiment spanned two consecutive years: the “ON” year, characterized by a high crop load, and the “OFF” year, marked by minimal fruit production. Key ecophysiological parameters, including sap flow, stomatal conductance, and photosynthetic rate, were monitored in both years. Pre-dawn water potential was measured using continuous stem psychrometers and the pressure chamber technique. Biochemical analyses focused on non-structural carbohydrate concentrations (starch, sucrose, and mannitol) and olive leaves’ carbon-stable isotope ratio (δ13C). Results revealed a higher leaf gas exchange rate during the “ON” year, leading to an average 29.3% increase in water consumption and a 40.78% rise in the photosynthetic rate. Higher water usage during the “ON” year resulted in significantly lower (43.22% on average) leaf water potential. Sucrose and starch concentrations were also increased in the “ON” year, while there were no significant differences in mannitol concentration. Regarding the carbon-stable isotope ratio, leaves from the “OFF” year exhibited significantly higher δ13C values, suggesting a higher resistance to the CO2 pathway from the atmosphere to carboxylation sites compared to the “ON” year plants.