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Lentinula edodes, one of the most popular, edible mushroom species with a high content of proteins and polysaccharides as well as unique aroma, is widely cultivated in many Asian countries, especially in China, Japan and Korea. As a white rot fungus with lignocellulose degradation ability, L. edodes has the potential for application in the utilization of agriculture straw resources. Here, we report its 41.8-Mb genome, encoding 14,889 predicted genes. Through a phylogenetic analysis with model species of fungi, the evolutionary divergence time of L. edodes and Gymnopus luxurians was estimated to be 39 MYA. The carbohydrate-active enzyme genes in L. edodes were compared with those of the other 25 fungal species, and 101 lignocellulolytic enzymes were identified in L. edodes, similar to other white rot fungi. Transcriptome analysis showed that the expression of genes encoding two cellulases and 16 transcription factor was up-regulated when mycelia were cultivated for 120 minutes in cellulose medium versus glucose medium. Our results will foster a better understanding of the molecular mechanism of lignocellulose degradation and provide the basis for partial replacement of wood sawdust with agricultural wastes in L. edodes cultivation.

The study aimed to explore suitable substrates comprising locally available hardwood sawdusts for the cultivation of Shiitake (Lentinula edodes) in Lebanon. Sawdusts of oak (OS), maple (MAP), and eucalyptus (EUC) were used alone or in combination, supplemented equally by wheat bran (WB). Results showed that complete mycelia run, fruiting, and harvest dates were the minimum in OS-WB: 800-200 by 72.2, 75.5, and 79.5 days after spawning (DAS) respectively, and the maximum in EUC-MAP-WB: 400-400-200 (by 88.3, 87.5, and 92.0 DAS, respectively). The substrate EUC-OS-WB: 400-400-200 had the highest biological efficiency (74.1%) compared to all treatments. Mushroom numbers ranged between 13.0 and 29.5 at harvest 1 (H1) and between 9.5 and 26.5 at harvest 2 (H2), showing a significant decrease in H2 in comparison to H1 in all treatments. Mushroom weight ranged between 8.8 and 25.9 at H1 and between 5.9 and 14.6 at H2. Furthermore, stepwise correlation showed that total biological yield (TBY) was positively affected by the biological yield at first harvest (BYH1) in OS-WB: 800-200 (R.sup.2 = 0.943), and at BYH2 in EUC-WB:800-200 (R.sup.2 = 0.944) and MAP-WB: 800-200 (R.sup.2 = 0.998), and it was negatively affected by BYH1 and stipe diameter in MAP-OS-WB: 400-400-200 (R.sup.2 = 0.946). Also, there was an improvement in mushroom protein, crude fibers, and vitamin C contents, and a decrease in carbohydrate contents on most substrates compared to control. Mushrooms obtained in EUC-OS-WB:400-400-200 recorded the highest protein and crude fiber contents (15.1 and 5.4%). Therefore, the mixture containing oak and eucalyptus sawdust has a good potential to improve shiitake yield and nutritional value compared to oak sawdust and could be an appropriate alternate for producing shiitake mushrooms.

To address the challenges of high labor intensity and low harvesting efficiency in shiitake mushroom production and management, this article presents a novel detection and classification method based on mamba-YOLO. This method adheres to the picking standards and grade specifications for shiitake mushrooms, enabling the automatic detection and quality grading of the mushrooms. Experiments conducted on a self-constructed shiitake mushroom dataset demonstrate that mamba-YOLO achieves precision (P), recall (R), mean average precision calculated at an IoU threshold of 50% (mAP@0.5), and average precision computed over IoU thresholds ranging from 50% to 95% in increments of 5% (mAP@0.5–0.95) of 98.89%, 98.79%, 97.86%, and 89.97%. The classification accuracies for various categories—mushroom stick, plane-surface immature, plane-surface mature, cracked-surface immature, cracked-surface mature, deformed mature, and deformed immature shiitake mushrooms—are 98.1%, 98.3%, 98.2%, 98.8%, 98.5%, 96.2%, and 96.9%. These results indicate that the proposed detection and grading method effectively determines the maturity of shiitake mushrooms and categorizes them based on cap texture characteristics. The network detection speed of 8.3 ms falls within the acceptable range for real-time applications, and the model’s parameters are compact at 6.1 M, facilitating easy deployment and scalability. Overall, the lightweight design, precise detection accuracy, and efficient detection speed of mamba-YOLO provide robust technical support for shiitake mushroom harvesting robots.

Mushrooms have been attracting attention as a source of bioactive compounds for the development of dietary supplements and medicines. Many researchers have reported pharmacological effects of edible mushrooms, and have isolated and identified bioactive substances. Lentinula edodes (shiitake) and Flammulina velutipes (enokitake) are the cultivated edible mushrooms that are popular throughout the world. In L. edodes, polyacetylenes and sulfur compounds have been shown to display antimicrobial activity. In F. velutipes, many types of bioactive terpenes have been reported from mycelium culture filtrate or solid culture substrate. This article reviews the bioactive metabolites of low-molecular weight from L. edodes and F. velutipes.

In this study, the biological applications of cultivation methods related to cultivar selection, vegetative growth, and reproductive development in Lentinula edodes cultivation are briefly reviewed to clarify the current situation and inform future developments. The current cultivars widely used in the main production areas are derived from wild strains distributed in northern Asia. The most effective techniques for cultivar identification are molecular markers identified in two nuclear genome datasets and one mitochondrial genome dataset. The current stage of cultivar breeding is at the junction of Breeding 3.0 (biological breeding) and Breeding 4.0 (intelligent breeding). Plant breeder’s rights and patents have different emphases on new breeding variety protection, with the former being the most utilized globally. L. edodes is mostly produced on synthetic logs filled with sawdust substrates. Hardwood sawdust comprises approximately 80% of the substrates. The vegetative growth of L. edodes on synthetic logs involves two distinct stages of mycelial colonization and browning. Mycelia mainly perform glycolysis, tricarboxylic acid cycle, and respiratory metabolism reactions to produce energy and intermediates for synthesizing the structural components of hyphae in the vegetative colonization stage. Upon stimulation by physiological and environmental pressures after colonization, mycelia trigger gluconeogenesis, autophagy, and secondary metabolism, increase metabolic flux of pentose phosphate pathway, activate the glyoxylate cycle, and accumulate melanin on the surface of logs to ensure growth and survival. Sexually competent mycelia can form hyphal knots as a result of reprogrammed hyphal branching patterns after a period of vegetative growth (which varies by cultivar) and stimulation by specific environmental factors. Under a genetically encoded developmental program, hyphal knots undergo aggregation, tissue differentiation, primordium formation, meiosis in the hymenium, stipe elongation, basidiospore production and maturation, and cap expansion to form mature fruiting bodies. Growers can achieve good fruiting body shape and high yield by regulating the number of young fruiting bodies and adjusting specific environmental factors. Key points • Cultivar selection becomes less with the increasing technological requirement of L. edodes cultivation. • L. edodes mycelia showed different biological events in the mycelial colonization and browning stages. • Specific cultivar breading may be the next milestone in L. edodes cultivation.

In this study, we examined aqueous extracts of the edible mushrooms Pleurotus ostreatus (oyster mushroom) and Lentinula edodes (shiitake mushroom). Proteome analysis was conducted using LC-Triple TOF-MS and showed the expression of 753 proteins by Pleurotus ostreatus, and 432 proteins by Lentinula edodes. Bioactive peptides: Rab GDP dissociation inhibitor, superoxide dismutase, thioredoxin reductase, serine proteinase and lectin, were identified in both mushrooms. The extracts also included promising bioactive compounds including phenolics, flavonoids, vitamins and amino acids. The extracts showed promising antiviral activities, with a selectivity index (SI) of 4.5 for Pleurotus ostreatus against adenovirus (Ad7), and a slight activity for Lentinula edodes against herpes simplex-II (HSV-2). The extracts were not cytotoxic to normal human peripheral blood mononuclear cells (PBMCs). On the contrary, they showed moderate cytotoxicity against various cancer cell lines. Additionally, antioxidant activity was assessed using DPPH radical scavenging, ABTS radical cation scavenging and ORAC assays. The two extracts showed potential antioxidant activities, with the maximum activity seen for Pleurotus ostreatus (IC50 µg/mL) = 39.46 ± 1.27 for DPPH; 11.22 ± 1.81 for ABTS; and 21.40 ± 2.20 for ORAC assays. This study encourages the use of these mushrooms in medicine in the light of their low cytotoxicity on normal PBMCs vis à vis their antiviral, antitumor and antioxidant capabilities.

High-throughput Illumina RNA-seq was used for deep sequencing analysis of the transcriptome of poly(A)+ RNA from mycelium grown under three different conditions: 30 days darkness (sample 118), 80 days darkness (313W), and 30 days darkness followed by 50 days in the light (313C), in order to gain insight into the molecular mechanisms underlying the process of light-induced brown film (BF) formation in the edible mushroom, Lentinula edodes . Of the three growth conditions, BF formation occurred in 313C samples only. Approximately 159.23 million reads were obtained, trimmed, and de novo assembled into 31,511 contigs with an average length of 1,746 bp and an N 50 of 2,480 bp. Based on sequence orientations determined by a BLASTX search against the NR, Swiss-Prot, COG, and KEGG databases, 24,246 (76.9 %) contigs were assigned putative descriptions. Comparison of 313C/118 and 313C/313W expression profiles revealed 3,958 and 5,651 significantly differentially expressed contigs (DECs), respectively. Annotation using the COG database revealed that candidate genes for light-induced BF formation encoded proteins linked to light reception (e.g., WC-1, WC-2, phytochrome), light signal transduction pathways (e.g., two-component phosphorelay system, mitogen-activated protein kinase pathway), and pigment formation (e.g., polyketide synthase, O -methyltransferase, laccase, P 450 monooxygenase, oxidoreductase). Several DECs were validated using quantitative real-time polymerase chain reaction. Our report is the first to identify genes associated with light-induced BF formation in L. edodes and represents a valuable resource for future genomic studies on this commercially important mushroom.