Journal Article

Proteome Analysis and In Vitro Antiviral, Anticancer and Antioxidant Capacities of the Aqueous Extracts of Lentinula edodes and Pleurotus ostreatus Edible Mushrooms

Awad, Mohamed F., Elleboudy, Nooran S., Yassein, Mahmoud A., Aboshanab, Khaled M., El-Mahdy, Taghrid S., Farag, Mohamed M. S., Elhusseiny, Shaza M.

2021

Overview

In this study, we examined aqueous extracts of the edible mushrooms Pleurotus ostreatus (oyster mushroom) and Lentinula edodes (shiitake mushroom). Proteome analysis was conducted using LC-Triple TOF-MS and showed the expression of 753 proteins by Pleurotus ostreatus, and 432 proteins by Lentinula edodes. Bioactive peptides: Rab GDP dissociation inhibitor, superoxide dismutase, thioredoxin reductase, serine proteinase and lectin, were identified in both mushrooms. The extracts also included promising bioactive compounds including phenolics, flavonoids, vitamins and amino acids. The extracts showed promising antiviral activities, with a selectivity index (SI) of 4.5 for Pleurotus ostreatus against adenovirus (Ad7), and a slight activity for Lentinula edodes against herpes simplex-II (HSV-2). The extracts were not cytotoxic to normal human peripheral blood mononuclear cells (PBMCs). On the contrary, they showed moderate cytotoxicity against various cancer cell lines. Additionally, antioxidant activity was assessed using DPPH radical scavenging, ABTS radical cation scavenging and ORAC assays. The two extracts showed potential antioxidant activities, with the maximum activity seen for Pleurotus ostreatus (IC50 µg/mL) = 39.46 ± 1.27 for DPPH; 11.22 ± 1.81 for ABTS; and 21.40 ± 2.20 for ORAC assays. This study encourages the use of these mushrooms in medicine in the light of their low cytotoxicity on normal PBMCs vis à vis their antiviral, antitumor and antioxidant capabilities.

• Publisher: MDPI AG,MDPI
• Subject: Adenoviruses / Amino acids / Amino Acids - chemistry / Amino Acids - isolation & purification / Antihypertensives / Antineoplastic Agents - chemistry / Antineoplastic Agents - isolation & purification / Antineoplastic Agents - pharmacology / antioxidant / Antioxidants / Antioxidants - chemistry / Antioxidants - isolation & purification / Antioxidants - pharmacology / antitumor / Antiviral Agents - chemistry / Antiviral Agents - isolation & purification / Antiviral Agents - pharmacology / Antiviral drugs / Benzothiazoles - antagonists & inhibitors / Biphenyl Compounds - antagonists & inhibitors / Cancer / Carbohydrates / Cell Line, Tumor / Cell Survival - drug effects / Complex Mixtures - chemistry / Cytotoxicity / Flavonoids / Flavonoids - chemistry / Flavonoids - isolation & purification / Fungal Proteins - chemistry / Fungal Proteins - classification / Fungal Proteins - isolation & purification / Fungi / Herpes viruses / Humans / Lectins - chemistry / Lectins - isolation & purification / Lentinula edodes / Leukemia / Leukocytes, Mononuclear - cytology / Leukocytes, Mononuclear - drug effects / Lymphoma / Mushrooms / Organ Specificity / Phenols - chemistry / Phenols - isolation & purification / Picrates - antagonists & inhibitors / Pleurotus - chemistry / Pleurotus - metabolism / Pleurotus ostreatus / Primary Cell Culture / Proteins / Proteome - chemistry / Proteome - classification / Proteome - isolation & purification / Proteomics / Retention / Serine Proteases - chemistry / Serine Proteases - isolation & purification / shiitake / Shiitake Mushrooms - chemistry / Shiitake Mushrooms - metabolism / Sulfonic Acids - antagonists & inhibitors / Superoxide Dismutase - chemistry / Superoxide Dismutase - isolation & purification / Thioredoxin-Disulfide Reductase - chemistry / Thioredoxin-Disulfide Reductase - isolation & purification / Vitamins - chemistry / Vitamins - isolation & purification / Water - chemistry / white rot fungus