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This practical guide is intended for all violinists and viola players who wish to give - or to understand and appreciate - historically aware performances of early music for their instruments. It comprises discussion of the literature, history and repertory of the violin and viola, the myriad relevant primary sources and their interpretation, and the various aspects of style and technique that combine to make up well-grounded, period performances. It also considers various related family instruments, contains practical advice on the acquisition of appropriate instruments, and offers suggestions for further reading and investigation. Many of the principles outlined are put into practice in case studies of six works composed c.1700–c.1900, the core period which forms this series' principal (though not exclusive) focus. Music by Corelli, Bach, Haydn, Beethoven, Mendelssohn and Brahms is examined with a view to recreating performances as faithful as possible to the composer's original intention.

Este artigo tem como objetivo a aplicaçao de Coordination, o conjunto de técnicas e principios desenvolvidos pela violista Karen Tuttle, na interpretando de Brasiliana para Viola e Cordas de Edino Krieger. Os principios centrais de Coordination sao realizados através de movimentos físicos no corpo do instrumentista para conectar as intençöes emocionais e musicais da interpretando. Para esse estudo foi realizada uma entrevista interativa com a Profa. Susan Dubois, antiga assistente pedagógica de Tuttle na Juilliard School of Music, e uma das principais professoras dessas técnicas. Após uma breve revisao do histórico de Tuttle, suas técnicas, e da obra em questao, sao apresentados excertos da parte da viola solo com notaçöes de sugestÐes interpretativas aplicando essas técnicas.

Over the past two decades, microfluidic-based separations have been used for the purification, isolation, and separation of biomolecules to overcome difficulties encountered by conventional chromatography-based methods including high cost, long processing times, sample volumes, and low separation efficiency. Cyclotides, or cyclic peptides used by some plant families as defense agents, have attracted the interest of scientists because of their biological activities varying from antimicrobial to anticancer properties. The separation process has a critical impact in terms of obtaining pure cyclotides for drug development strategies. Here, for the first time, a mimic of the high-performance liquid chromatography (HPLC) on microfluidic chip strategy was used to separate the cyclotides. In this regard, silica gel-C18 was synthesized and characterized by Fourier-transform infrared spectroscopy (FTIR) and proton nuclear magnetic resonance (1H-NMR) and then filled inside the microchannel to prepare an HPLC C18 column-like structure inside the microchannel. Cyclotide extract was obtained from Viola ignobilis by a low voltage electric field extraction method and characterized by HPLC and matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF). The extract that contained vigno 1, 2, 3, 4, 5, and varv A cyclotides was added to the microchannel where distilled water was used as a mobile phase with 1 µL/min flow rate and then samples were collected in 2-min intervals until 10 min. Results show that cyclotides can be successfully separated from each other and collected from the microchannel at different periods of time. These findings demonstrate that the use of microfluidic channels has a high impact on the separation of cyclotides as a rapid, cost-effective, and simple method and the device can find widespread applications in drug discovery research.

Cyclotides are plant cyclic peptides with exceptional stability and diverse bioactivity, making them promising candidates for biomedical applications. Therefore, the study of cyclotides has attracted increasing attention in recent years. However, the existing cyclotide detection methods face limitations in sensitivity, accuracy, and reliability. To address these challenges, we developed an integrated strategy using a combination of strong cation exchange chromatography techniques for removing interfering small molecules, Orbitrap Exploris 480 mass spectrometry (OEMS); this is a detection and database searching-based method for cyclotide verification, which greatly improved the sensitivity, accuracy, and reliability of cyclotide identification. This strategy was subsequently employed for cyclotide mapping in Viola with a minute amount of starting tissue, resulting the identification of 65 known and 18 potentially novel cyclotides, which is the largest dataset of cyclotides for Viola philippica. This strategy provided valuable insights into the cyclotide diversity and distribution in V. philippica, with potential applications in drug discovery and other biomedical fields.

Studies of concert life in nineteenth-century America have generally been limited to large orchestras and the programs we are familiar with today. But as this book reveals, audiences of that era enjoyed far more diverse musical experiences than this focus would suggest. To hear an orchestra, people were more likely to head to a beer garden, restaurant, or summer resort than to a concert hall. And what they heard weren't just symphonic works—programs also included opera excerpts and arrangements, instrumental showpieces, comic numbers, and medleys of patriotic tunes. This book brings together musicologists and historians to investigate the many orchestras and programs that developed in nineteenth-century America. In addition to reflecting on the music that orchestras played and the socioeconomic aspects of building and maintaining orchestras, the book considers a wide range of topics, including audiences, entrepreneurs, concert arrangements, tours, and musicians' unions. The authors also show that the period saw a massive influx of immigrant performers, the increasing ability of orchestras to travel across the nation, and the rising influence of women as listeners, patrons, and players. Painting a rich and detailed picture of nineteenth-century concert life, this collection will greatly broaden our understanding of America's musical history.

Mosquitoes and mosquito-borne diseases represent an increasing global challenge. Plant extract and/or oils could serve as alternatives to synthetic insecticides. The larvicidal effects of 32 oils (1000 ppm) were screened against the early 4th larvae of Culex pipiens and the best oils were evaluated against adults and analyzed by gas chromatography-mass spectrometry (GC mass) and HPLC. All oils had larvicidal activity (60.0–100%, 48 h Post-treatment, and their Lethal time 50 (LT 50 ) values ranged from 9.67 ( Thymus vulgaris ) to 37.64 h ( Sesamum indicum ). Oils were classified as a highly effective group (95–100% mortalities), including Allium sativum, Anethum graveolens, Camellia sinensis, Foeniculum vulgare, Nigella sativa, Salvia officinalis, T. vulgaris, and Viola odorata. The moderately effective group (81–92% mortalities) included Boswellia serrata, Cuminum cyminum, Curcuma aromatic, Allium sativum, Melaleuca alternifolia, Piper nigrum, and Simmondsia chinensis . The least effective ones were C. sativus and S. indicum. Viola odorata, Anethum graveolens, T. vulgaris, and N. sativa provide 100% adult mortalities PT with 10, 25, 20, and 25%. The mortality percentages of the adults subjected to 10% of oils (H group) were 48.89%, 88.39%, 63.94%, 51.54%, 92.96%, 44.44%, 72.22%, and 100% for A. sativum, An. graveolens, C. sinensis, F. vulgare, N. sativa, S. officinalis, T. vulgaris, and V. odorata, respectively. Camellia sinensis and F. vulgare were the most potent larvicides whereas V. odorata, T. vulgaris, An. graveolens and N. sativa were the best adulticides and they could be used for integrated mosquito control.

Viola L. is a botanical genus with approximately 525 to 620 species, spread worldwide. Several violets are traditionally used as edible flowers and have been recently proved to be a source of bioactive compounds, including flavonols, flavanols, benzoic acids, and cinnamic acids. However, no information is available about the phytochemical profile of the Viola calcarata complex, which is found in the Alpine environment. Thus, the present research aimed to assess the antioxidant activity and the presence of bioactive compounds (anthocyanins and phenolic compounds) in V. calcarata subspecies, to promote their biodiversity and use in the agrifood sector. Two V. calcarata subspecies were chosen, with different colors: V. calcarata subspecies calcarata L., with white (CW), yellow (CY), and violet flowers (CV); and V. calcarata subspecies villarsiana (Roem & Schult.) Merxm., with bicolor (violet and yellow—VB) flowers. CY showed a significantly higher phenolic content (1116.43 mg GAE 100 g−1 FW) than the other subspecies, while CV showed higher values in anthocyanins content (44.73 mg C3G 100 g−1 FW). Regarding the antioxidant activity, CW (215.07 mmol Fe2+ kg−1 FW, 99.53 µmol TE g−1 FW, and 32.30 µmol TE g−1 FW for FRAP, DPPH, and ABTS, respectively) and VB (217.33 mmol Fe2+ kg−1 FW, 90.97 µmol TE g−1 FW, and 29.17 µmol TE g−1 FW for FRAP, DPPH, and ABTS, respectively) showed the highest values. Through HPLC, a total of eight phenolic compounds were quantitatively identified among the two subspecies, including flavonols, cinnamic acids, benzoic acids, catechins, and vitamin C. Though different in their composition, the two subspecies are rich in phenolic compounds, highlighting the importance of preserving their biodiversity and their potential use in the agrifood sector.

Accurate freckle segmentation is essential for dermatological assessments and cosmetic applications, but existing lesion detection techniques are primarily designed for well-defined skin abnormalities such as melanomas and tumors, making them less effective at capturing subtle features like freckles. In this study, we present an automated freckle segmentation framework that integrates the Gaussian Mixture Model (GMM) and the Viola-Jones algorithm for skin segmentation, coupled with an energy map-based approach for freckle detection. The process begins with image is clustered using GMM, followed by facial region detection with the Viola-Jones algorithms. A post-processing step then segments the selection of the skin region. Subsequently, an energy map is generated by combining the blue and saturation channels, while Contrast-Limited Adaptive Histogram Equalization (CLAHE) and morphological operations enhance freckle contrast. The final segmentation is achieved through binarization and additional post-processing techniques. Quantitative evaluations demonstrate that the proposed method surpasses conventional approaches in recall, Intersection over Union (IoU), and Dice coefficient, highlighting its effectiveness in accurate freckle detection and segmentation. These findings indicate that, with further refinement, the proposed framework holds significant potential for applications in both clinical dermatology and cosmetic science.

Violae herba (Zihuadiding) is a common Chinese herbal medicine. The current Chinese Pharmacopeia (ChP) defines Viola yedoensis Makino as the sole plant origin and esculetin as its sole quality-marker (Q-marker). Esculetin, however, occurs in some counterfeits as well. This implies that current ChP criterion actually cannot recognize the counterfeits of V. yedoensis . The study thus created a specialized MS library using ultra-high-performance liquid chromatography with quadrupole-orbitrap tandem mass spectrometry (UHPLC- Q -Orbitrap-MS/MS) analysis. Through library-comparison, 36 compounds were putatively identified from V. yedoensis ; especially, four isomers were successfully distinguished, that is, vitexin vs isovitexin and schaftoside vs isoschaftoside. The subsequent UHPLC- Q -Orbitrap-MS/MS semi-quantification suggested that the chemical contents of 36 compounds varied from 0.001 to 1.958% and the old Q-marker esculetin had high content (0.484 ± 0.028%). According to the relevant principles, flavonoid luteolin was proposed as the new and additional Q-marker. The proposal offers a preliminary evidence to recognize seven adulterations (or counterfeits) of V. yedoensis .

The therapeutic potential of plant-derived polyphenols has garnered significant attention due to their roles in modulating inflammation and cancer progression. Viola odorata ( V. odorata ), traditionally used in herbal medicine, is known for its bioactive constituents, yet comprehensive profiling of its floral phytochemicals—particularly phenolic acids, flavonoids, and anthocyanins—remains limited. This study was pharmacologically designed to investigate the detailed phytochemical composition of V. odorata flowers and evaluate their anti-inflammatory and anticancer activities, aiming to identify novel natural agents for therapeutic development. Methanolic extracts of V. odorata flowers were analyzed for total phenolics, flavonoids, and anthocyanins using spectrophotometric methods. HPLC and UPLC/ESI–MS techniques were employed to identify and quantify individual compounds. Structural elucidation of isolated flavonoids was performed using advanced spectroscopic techniques. The anti-inflammatory and cytotoxic effects of the extract and isolated flavonoids were assessed in vitro using hepatocellular carcinoma (HepG2), human colonic epithelial (Caco-2), and colorectal carcinoma (HTC-116) cell lines. Molecular dynamics simulations were conducted to explore the interactions underlying the observed bioactivities. The methanolic extract contained 81.34 ± 0.17 mg GAE/g of total phenolics, 69.45 ± 0.24 mg CE/g of flavonoids, and 92.43 mg cyanidin-3-glucoside/100 g of anthocyanins. HPLC analysis revealed twelve phenolic acids and ten flavonoids, with gentisic acid (391.37 μg/g), apigenin-7-glucoside (417.22 μg/g), catechin (372.56 μg/g), and rutin (262.73 μg/g) being predominant. UPLC/ESI–MS identified 8 phenolic acids and derivatives, 3 flavonols, 4 flavones, 14 flavonoid glycosides, and 5 anthocyanins derived from cyanidin, delphinidin, and petunidin. Three flavonoids—5,7-dihydroxy-3,6-dimethoxyflavone, luteolin 7- O -glucoside, and kaempferol 3- O -rutinoside—were isolated and structurally characterized. The extract and isolated compounds exhibited dose-dependent cytotoxicity and anti-inflammatory effects across all tested cell lines. Molecular dynamics studies supported the observed inhibitory mechanisms. This study highlights the rich polyphenolic profile of V. odorata flowers and their promising anti-inflammatory and anticancer properties. The correlation between phytochemical composition and pharmacological activity underscores the therapeutic potential of V. odorata as a source of bioactive compounds for drug development.