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"germplasm conservation"
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Paclobutrazol in the in vitro conservation of cassava genotypes
In vitro germplasm conservation allows to extend the interval between subcultures without compromising the viability and genetic integrity of the plant, ensuring a backup of genotypes with high phytosanitary quality. Thus, this study aimed to verify the effect of four concentrations of Paclobutrazol® in inducing minimum growth in five Manihot esculenta accessions from the in vitro Active Germplasm Bank of Embrapa Cassava and Fruits. An experiment was installed using the Murashige and Skoog medium without addition and added with four concentrations of Paclobutrazol® (0.10, 0.20, 0.30, and 0.40 mg L-1), in five in vitro accessions of M. esculenta: BRS Jari (BGM 2041), Cigana (BGM 0264), BRS Poti Branca (BGM 2017), TME 14, and BRS Novo Horizonte. The statistical design was completely randomized in a 5 x 5 factorial scheme, with 15 repetitions. After 120 days of cultivation, the following variables were evaluated: plant height (cm), number of green leaves, number of senescent leaves, number of mini-cuttings, number of shoots, and fresh and dry mass of shoots and roots (mg). Paclobutrazol® caused a reduction in plant height and gain in root mass for all accessions, in addition to preserving the number of green leaves and decreasing leaf senescence for most genotypes. There was a strong dependence of the genotype in relation to the concentration of Paclobutrazol®. The concentration of 0.20 mg L-1 showed potential in the in vitro conservation of M. esculenta genotypes.
Journal Article
Molecular Viral Diagnosis and Sanitation of Yam Genetic Resources: Implications for Safe Yam Germplasm Exchange
Yam (Dioscorea spp.) is an important crop in tropical and subtropical regions. Many viruses have been recently identified in yam, hampering genetic conservation and safe international exchanges of yam germplasm. We report on the implementation of reliable and cost-effective PCR-based detection tools targeting eight different yam-infecting viruses. Viral indexing of the in vitro yam collection maintained by the Biological Resources Center for Tropical Plants (BRC-TP) in Guadeloupe (French West Indies) unveiled a high prevalence of potyviruses, badnaviruses, Dioscorea mosaic associated virus (DMaV) and yam asymptomatic virus 1 (YaV1) and a high level of coinfections. Infected yam accessions were subjected to a combination of thermotherapy and meristem culture. Sanitation levels were monitored using PCR-based and high-throughput sequencing-based diagnosis, confirming the efficacy and reliability of PCR-based detection tools. Sanitation rates were highly variable depending on viruses. Sixteen accessions were successfully sanitized, paving the way to safe yam germplasm exchanges and the implementation of clean seed production programs worldwide.
Journal Article
Heirloom seeds and their keepers : marginality and memory in the conservation of biological diversity
Farmers and gardeners have long appreciated a wide variety of plants and have nurtured them for meals, medicine, and exchange. But diversity too often has been surrendered to monocultures of fields and spirits, predisposing much of modern agriculture to uniformity and, consequently, vulnerability. Today it is primarily at the individual level - such as growing and saving a strange old bean variety or a curious-looking gourd - that any lasting conservation actually takes place. Heirloom Seeds and Their Keepers offers a much-needed, scientifically researched perspective on the contribution of seedsaving that illustrates its critical significance to the preservation of both cultural knowledge and crop diversity around the world. It opens new conversations between anthropology and biology, and between researchers and practitioners, as it honors conservation as a way of life.
Book
Plant cryopreservation: a continuing requirement for food and ecosystem security
This issue of In Vitro Cellular and Developmental Biology—Plant is dedicated to current developments in liquid-nitrogen cryopreservation methods and their use in plant biology and germplasm preservation. The development of cryopreservation for storage of plant cells, tissues, and organs began in the 1960s and continues to this day. Long-term storage of in vitro cultures of secondary metabolite cell cultures, embryogenic cultures, clonal germplasm, endangered species, and transgenic products remains an important requirement for many scientists, organizations, and companies. The continued development of cryopreservation techniques and their application to new plants is the subject of this issue. 1980; Kartha et al. 1982; Towill 1983) to evolve and provide successful storage of the shoot tips of temperate plants (Reed and Hummer 1995; Sakai 1995; Reed and Chang 1997). Further improvements, including the development of various forms of vitrification techniques, resulted in the expansion of cryopreservation to a wider range of plant types and tissues that continue today (Fabre and Dereuddre 1990; Niino et al. 1992, 1997; Engelmann 2004; Staats et al. 2006, 2008; Yamamoto et al. 2011; Engelmann 2011; Engelmann and Dussert 2013).
Journal Article
Genetic resources and traditional knowledge : case studies and conflicting interests
This study describes efforts to define and protect traditional knowledge and the associated issues of access to genetic resources, from the negotiation of the Convention on Biological Diveristy through to the Declaration of Rights of Indigenous Peoples and the Nagoya Protocol.
Book
All Populations Matter: Conservation Genomics of Australia’s Iconic Purple Wattle, Acacia purpureopetala
Maximising genetic diversity in conservation efforts can help to increase the chances of survival of a species amidst the turbulence of the anthropogenic age. Here, we define the distribution and extent of genomic diversity across the range of the iconic but threatened Acacia purpureopetala, a beautiful sprawling shrub with mauve flowers, restricted to a few disjunct populations in far north Queensland, Australia. Seed production is poor and germination sporadic, but the species occurs in abundance at some field sites. While several thousands of SNP markers were recovered, comparable to other Acacia species, very low levels of heterozygosity and allelic variation suggested inbreeding. Limited dispersal most likely contributed towards the high levels of divergence amongst field sites and, using a generalised dissimilarity modelling framework amongst environmental, spatial and floristic data, spatial distance was found to be the strongest factor explaining the current distribution of genetic diversity. We illustrate how population genomic data can be utilised to design a collecting strategy for a germplasm conservation collection that optimises genetic diversity. For this species, inclusion of all field sites will capture maximum genetic diversity for both in situ and ex situ conservation. Assisted cross pollination, within and between field sites and genetically structured groups, is recommended to enhance heterozygosity particularly at the most disjunct sites and further fragmentation should be discouraged to avoid loss of genetic connectivity.
Journal Article
Cryopreservation of 13 Commercial Cannabis sativa Genotypes Using In Vitro Nodal Explants
Cannabis has developed into a multi-billion-dollar industry that relies on clonal propagation of elite genetics with desirable agronomic and chemical phenotypes. While the goal of clonal propagation is to produce genetically uniform plants, somatic mutations can accumulate during growth and compromise long-term genetic fidelity. Cryopreservation is a process in which tissues are stored at cryogenic temperatures, halting cell division and metabolic processes to facilitate high fidelity germplasm preservation. In this study, a series of experiments were conducted to optimize various stages of cryopreservation and develop a protocol for long-term germplasm storage of Cannabis sativa. The resulting protocol uses a standard vitrification procedure to cryopreserve nodal explants from in vitro shoots as follows: nodes were cultured for 17 h in a pre-culture solution (PCS), followed by a 20-min treatment in a loading solution (LS), and a 60 min incubation in plant vitrification solution 2 (PVS2). The nodes were then flash frozen in liquid nitrogen, re-warmed in an unloading solution at 40 °C, and cultured on basal MS culture medium in the dark for 5 days followed by transfer to standard culture conditions. This protocol was tested across 13 genotypes to assess the genotypic variability. The protocol was successful across all 13 genotypes, but significant variation was observed in tissue survival (43.3–80%) and regrowth of shoots (26.7–66.7%). Plants grown from cryopreserved samples were morphologically and chemically similar to control plants for most major traits, but some differences were observed in the minor cannabinoid and terpene profiles. While further improvements are likely possible, this study provides a functional cryopreservation system that works across multiple commercial genotypes for long-term germplasm preservation.
Journal Article
Conserving plant genetic diversity in protected areas: population management of crop wild relatives
Conservation in protected areas has focused on preserving biodiversity of ecosystems and species, whereas conserving the genetic diversity contained within species has historically often been ignored. However, maintaining genetic diversity is fundamental to food security and the provision of raw materials and it is best preserved within plants' natural habitats. This is particularly true for wild plants that are directly related to crop species and can play a key role in providing beneficial traits, such as pest or disease resistance and yield improvement. These wild relatives are presently threatened due to processes of habitat destruction and change and methodologies have been adapted to provide in-situ conservation through the establishment of genetic reserves within the existing network of protected areas. Providing a long-awaited synthesis of these new methodologies, this book presents a practical set of management guidelines that can be used for the conservation of plant genetic diversity of crop wild relatives in protected areas.
eBook