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Wine is a product of human creativity, culture, and nature, and therefore it is an intriguing subject for scientific research and admiration. Among its many kinds, white wine stands out oenologically because of its diverse flavours, aromatic attributes, and capacity to mature. In this experiment, we established 3 different bud load levels (25%, 50%, and 75%) on the well-known white wine grape variety ‘Fetească Albă’. The sugar content significantly increased in the 50% and 76% groups, whereas there were no significant changes regarding titratable acid content, must quantity, and cluster weight.

The increasing demand for animal-products has led to an increasing demand for livestock feed. Using cover crop as green manure in orchards is an effective measure to improve fruit yield and quality. However, the effect of mowing cover forage crops as livestock feed on soil quality and crop production is unclear. Therefore, a 4-year field experiment, which included two treatments, was conducted in pear orchards in Luniao County, China: natural grass (NG) and planting and mowing forage crop ryegrass as livestock feed (MF). Under MF treatment, most soil nutrient content, especially alkalihydrolysable N (AN), total phosphate (TP), available phosphate (AP), and microbial biomass phosphate (MBP), had decreased significantly (P<0.05), while β-D-glucosidase (BG, C-cycle enzyme) and soil C limitation at 10-20 cm depth and P limitation at subsoil (20-40 cm) was increased. In addition, the soil bacterial community component in topsoil (0-10 cm and 10-20 cm) and fungal community component in topsoil and subsoil were changed in the MF treatment. Network analysis showed that MF treatment had a lower edge number in topsoil but the community edge numbers increased from 12794 in NG to 13676 in MF in subsoil. The average weight degree of the three soil layers in MF treatment were reduced, but the modularity had increased than that in NG. For crop production, MF treatment was 1.39 times higher in pear yield and titratable acids (AC) reduced from 0.19% to 0.13% compared with NG. These changes were more associated with the indicators at the subsoil, especially for TP, AN, pH, and F-NMDS1 (non-metric multidimensional scaling (NMDS) axis 1 of fungi). These results provide data support for the feasibility of planting and mowing forage crops as livestock feed on orchards as well as a new idea for the integration of crop and livestock.

Excessive phosphorus (P) fertilization has led to high soil P accumulation in pear orchards across China, increasing the risk of P loss while limiting economic returns. Orchard grassing has been proposed as a strategy to optimize soil P content and reduce P loss; however, its limited economic benefits have hindered widespread adoption. To address this, we developed a novel planting and mowing ryegrass (MF) system, integrating P loss mitigation with improved economic returns. A two-year field experiment was conducted in the Yangtze River Basin to assess the effects of this system on soil P fractions, P loss risk, and pear production. The results showed that soil available nitrogen (N), available potassium (K), and total P content were significantly lower in the MF treatment compared to natural grassing (NG) at different growth stages. Moreover, the MF treatment increased pear yield by 14.7–16.7% and reduced titratable acidity by 23.5–47.1%, with these improvements primarily driven by changes in phosphorus-related indicators (NaOH-Pi, NaHCO3-Pi, and intermediate P) across different years. Additionally, the reduction in NaHCO3-Pi in the MF treatment contributed to a decline in P leaching risk indicators, including Olsen-P and CaCl2-P. These findings highlight the potential of the MF system as a sustainable orchard management strategy, effectively optimizing soil P dynamics, mitigating P leaching risks, and enhancing pear yield and quality under high P conditions.

Abstract Small fruits such as strawberries, are a good source of natural antioxidants. In recent decades, many efforts have been made to increase the shelf life of strawberries and maintain its nutritional value in post-harvest conditions. In the present study, the effects of spermine (Spm) and spermidine (Spd) (0, 1.0 and 1.5 mM) on the post-harvest life and quality of strawberry fruits during the 3rd, 6th, and 12th days of storage, were investigated. Applications of Spm and Spd decreased the rate of weight loss, fruit decay, soluble solids content, fruit juice pH and taste index during the storage period in compared to the control. However, titratable acids and vitamin C contents, tissue stiffness, phenolic compounds and antioxidant activity increased in compared to the control. These growth regulators prevented the aging and loss of bioactive compounds of the fruit by increasing the antioxidant activity and preventing the destruction of the fruit tissue. Among the studied treatments, applications of 1.5 mM of Spm and Spd were the most effective treatments to enhance the storage life and quality characters of strawberry fruits. RESUMO Frutas pequenas como morangos são uma boa fonte de antioxidantes naturais. Nas últimas décadas, muitos esforços têm sido feitos para aumentar sua vida útil e manter seu valor nutricional em condições de pós-colheita. No presente estudo, foram investigados os efeitos da espermina (Spm) e espermidina (Spd) (0, 1,0 e 1,5 mm) pós-colheita e na qualidade dos frutos de morango durante o 3º, 6º e 12º dias de armazenamento. Aplicações de Spm e Spd diminuíram a taxa de perda de peso, podridão dos frutos, teor de sólidos solúveis, pH do suco de frutas e índice de sabor durante o período de armazenamento em comparação com o controle. No entanto, os teores de ácidos tituláveis e vitamina C, rigidez tecidual, compostos fenólicos e atividade antioxidante aumentaram em relação ao controle. Esses reguladores de crescimento preveniram o envelhecimento e a perda de compostos bioativos da fruta, aumentando a atividade antioxidante e evitando a destruição do tecido da fruta. Entre os tratamentos estudados, as aplicações de 1,5 mm de Spm e Spd foram os tratamentos mais eficazes para aumentar a vida de armazenamento e as características de qualidade dos frutos de morango.

As a local medicine and food, wampee fruit, with abundant bioactive compounds, is loved by local residents in Southern China. Titratable acid (TA), total sugar (TS), and total phenolic and flavonoid contents were detected, and phytochemical profiles and cellular antioxidant activities were analyzed by the HPLC and CAA (cellular antioxidant activity) assay in five sweet wampee varieties and five sour wampee varieties. Results showed that the average TS/TA ratio of sweet wampee varieties was 29 times higher than sour wampee varieties, while TA content was 19 times lower than sour wampee varieties. There were much lower levels of total phenolics, flavonoids, and antioxidant activities in sweet wampee varieties than those in sour wampee varieties. Eight phytochemicals were detected in sour wampee varieties, including syringin, rutin, benzoic acid, 2-methoxycinnamic acid, kaempferol, hesperetin, nobiletin, and tangeretin, while just four of them were detected in sweet wampee varieties. Syringin was the only one that was detected in all the sour wampee varieties and was not detected in all sweet wampee varieties. Correlation analysis showed significant positive correlations between TA with phenolics, flavonoids, and total and cellular (PBS wash) antioxidant activities, while there were significant negative correlations between TS/TA with phenolic and cellular (no PBS wash) antioxidant activities. This suggested that the content of titratable acid in wampee fruit might have some relationship with the contents of phenolics and flavonoids. Sour wampee varieties should be paid much attention by breeders for their high phytochemical contents and antioxidant activities for cultivating germplasms with high health care efficacy.

Cleft grafting methods were applied in order to evaluate the effect of grafting on tomato fruit yield and quality. The tomato cultivars used as scions were Yeni Talya, Swanson and Beri. The cultivars Beaufort and Arnold were used as rootstocks. The following characteristics of grafted and nongrafted plants were recorded: fruit index, number of fruits/truss, fruit weight, fruit yield, dry matter, pH, concentration of soluble solids, titratable acidity, concentrations of total sugar, lycopene and vitamin C. The results showed that fruit yield and fruit index, number of fruits/truss and fruit weights were improved by grafting. Fruit quality, measured in terms of concentrations of dry matter, soluble solids, total sugar, and vitamin C, was lower in grafted plants than in nongrafted ones. No significant difference in lycopene level and pH was found. Titratable acidity was improved by grafting. A positive effect of grafting was recorded when Beaufort was used as rootstock. These results showed that grafting could be an advantageous alternative in tomato production.

Peach (Prunus persica (L.)) fruits are abundant in nutrients, with fruit shape and sugar content serving as critical indicators of fruit quality. Melatonin plays a pivotal role in peach fruit development; however, the mechanisms by which it regulates fruit shape development, sugar metabolism, and secondary metabolites remain largely unknown. In this study, peach trees were sprayed with 150 µM melatonin 20 days after pollination. Traditional methods were used to investigate fruit morphology, total soluble solids (TSSs), and titratable acidity content (TAC), while liquid chromatography–mass spectrometry (LC-MS) was employed to analyze sugar metabolites during fruit development. The results indicated that melatonin treatment augmented the transverse and longitudinal diameters of peach fruits by 12% and 6%, respectively, and elevated the contents of soluble solids and titratable acid by 7% and 6%, respectively. The single fruit weight experienced a significant increase of 29.4%, whereas fruit firmness at maturity remained unchanged. Metabolite analysis demonstrated that melatonin decreased the levels of sucrose and D-sorbitol in mature fruits but enhanced the accumulation of D-fructose, L-rhamnose, and xylose. Significantly, melatonin expedited the degradation of galactose, D-mannose, and methyl-D-pyranogalactoside prior to maturity (all three substances naturally decline with fruit ripening), highlighting its role in promoting fruit ripening. In conclusion, exogenous melatonin improves the internal nutrition and flavor quality of fruit by regulating the accumulation of primary and secondary metabolites during fruit ripening. Specifically, the increase in D-fructose (a major contributor to sweetness) and L-rhamnose (a potential precursor for aroma compounds) enhances fruit flavor profile. The accelerated degradation of galactose, D-mannose, and methyl-D-pyranogalactoside (components of cell wall polysaccharides) prior to maturity, alongside the metabolic shift favoring fructose accumulation over sucrose, highlights melatonin’s role in promoting fruit ripening and softening processes. It also promotes fruit enlargement and single fruit weight without affecting fruit firmness. This study establishes a theoretical basis for the further investigation of the molecular mechanisms underlying melatonin’s role in peach fruits and for enhancing quality-focused breeding practices.

A triennial evaluation of the content of titratable acids (TA) in grain was carried out on 12 varieties of proso millet (Panicum miliaceum L.). The model variety, Mironovskoe 94, harvested in 2004, was used for the monitoring of sensory changes in TA content, in relationship to different storage conditions and the application of different grain processing methods. The Czech state norm (CSN 56 0512-9 1995) Determination of Titratable Acids, with a slight modification, was used as the standard method. The TA contents in dry matter varied between 39 and 78 mmol/kg of grain dry matter, and significant differences between varieties and experimental years were found. The method of proso millet processing had a key role in the final sensitivity to rancidity. The storage conditions had a much lower influence on TA content than the processing technology. The clearly detected sensory changes found in scoured grains corresponded with TA contents within the range of 90 to106 mmol/kg. The monitoring of the TA content can be considered as a suitable tool for the prediction of rancidity processes in millet grains.

We fed common brushtail possums artificial diets containing a buffer and the plant secondary metabolite (PSM), orcinol, to test the hypothesis that organic acids, common products of PSM metabolism, limit feeding by common brushtail possums ( Trichosurus vulpecula ). We introduced several diets containing orcinol and a buffer (urinary alkalising agent) over a course of three experiments. A diet containing 2% orcinol (wet matter) caused possums to reduce their food intake immediately, but feeding returned to normal 1–2 days later. Even though possums excreted strongly acidic urine (pH 5.1) and had perturbed nitrogen metabolism, they maintained their food intake and body mass until the experiment terminated 9 days after the introduction of orcinol. Possums ate 52% less when the basal diet contained 4% orcinol. As expected, the acid loads caused a change in the composition of urinary nitrogen with possums excreting more ammonium than urea and a large amount of unidentified nitrogenous material. Supplementing the diet containing orcinol with buffer neutralised the metabolic acid load and partly restored normal nitrogen metabolism, but did not restore feeding. Also, animals eating orcinol excreted normal amounts of 3-methylhistidine, indicating no increase in muscle protein catabolism. This suggests that a limitation to the rate of detoxification or toxicosis, rather than acid loads, limits the ingestion of acid-inducing PSMs.

Nutrition studies suggest that blueberries help keep us healthy and these possible health benefits may be due to the antioxidant capacity as well as other natural compounds in blueberry fruit. Different taxa of Vaccinium species (Bilberry - Vaccinium myrtillus L.; lowbush blueberry - Vaccinium angustifolium Ait.; half-highbush blueberry - Vaccinium corymbosum x Vaccinium angustifolium ‘Northblue’ and ‘Northcountry’) were analyzed for surface color, size, sugars, titratable acidity, soluble solids, fat, protein, vitamins and anthocyanin content. The blueberry fruit were harvested from three commercial blueberry plantations and from one natural habitat in 2003. The fruit of V. angustifolium were collected from two different areas: one plantation was located on mineral soil and the other on peat soil (abandoned peat pits). The anthocyanin content ranged from 125 to 405 mg·100 g-1 of fresh berry in different species and cultivars. Half-highbush blueberry ‘Northblue’ had the largest berry size but ascorbic acid and anthocyanin contents were low. V. myrtillus had the lowest total energy value, but the highest anthocyanin content. Glucose and fructose content was higher in fruit of V. angustifolium compared with the other species. The soil type (mineral or peat soil) did not affect anthocyanin and ascorbic acid content of V. angustifolium fruit but titratable acids and moisture content was higher in fruit of V. angustifolium grown on peat soil, compared with mineral soil. Badania dotyczące dietetycznych właściwości borówki wskazują na prozdrowotny wpływ spożycia owoców na zdrowie człowieka ze względu na właściwości antyoksydacyjne oraz obecność innych naturalnych składników w owocach. Różne obiekty Vaccinium (czarna jagoda - Vaccinium myrtillus L.; borówka amerykańska niska - Vaccinium angustifolium AIT.; borówka amerykańska średniowysoka - Vaccinium corymbosum x Vaccinium angustifolium ‘Nortblue’ i Nortcountry') wzięto do oceny koloru powierzchni owocu, jego wielkości, zawartości cukrów, substancji nierozpuszczalnych, tłuszczy, białek witamin, antocyjanów oraz kwasowości miareczkowej. Owoce borówki były zbierane na plantacjach uprawnych i w warunkach naturalnych w 2003 roku. Owoce V. angustifolium zbierano na dwóch plantacjach: jedna była założona na glebie mineralnej a druga na torfowej. Zawartości antocyjanów wynosiła od 125 do 405 mg·100 g-1 sAwieżej masy w owocach różnych obiektów. Borówka amerykańska średniowysoka ‘Nortblue’ miała większe owoce lecz zawartość kwasu askorbinowego i antocyjanów była mniejsza. V. Myrtillus miała niższą wartość energetyczną lecz wyższą zawartość antocyjanów. Zawartość glukozy i fruktozy w owocach V. angustifolium była porównywalna z innymi obiektami. Rodzaj gleby (mineralna lub torfowa) nie miał wpływu na zawartość antocyjanów i kwasu askorbinowego w owocach V. angustifolium lecz zawartość kwasów i wody była wyższa w owocach V. angustitolium uprawianej na glebie torfowej w porównaniu z glebą mineralną.