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Randomized, placebo-controlled trial reveals the impact of dose and timing of Bifidobacterium infantis probiotic supplementation on breastfed infants’ gut microbiome
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Randomized, placebo-controlled trial reveals the impact of dose and timing of Bifidobacterium infantis probiotic supplementation on breastfed infants’ gut microbiome
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Randomized, placebo-controlled trial reveals the impact of dose and timing of Bifidobacterium infantis probiotic supplementation on breastfed infants’ gut microbiome
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Journal Article
Randomized, placebo-controlled trial reveals the impact of dose and timing of Bifidobacterium infantis probiotic supplementation on breastfed infants’ gut microbiome
2026
Overview
This study found that supplementing exclusively breastfed infants with a probiotic, Bifidobacterium infantis EVC001, between 2 and 4 months of age can successfully restore beneficial bacteria in their gut, even after the newborn period. Although previous research showed this effect in newborns, this is the first study to demonstrate that older infants, whose gut microbiomes are typically more stable, can still benefit. The probiotic was effective at all tested doses, with higher levels of B. infantis and overall Bifidobacteriaceae in infants' stool during and even 1 month after supplementation. This study demonstrates that B. infantis can take hold in the gut and potentially improve gut health in older breastfed babies, offering a promising approach to support infant health in settings where beneficial gut bacteria are often missing. This study was registered at clinicaltrials.gov as NCT03476447 .
Publisher
American Society for Microbiology,American Society for Microbiology (ASM)
Subject
/ Bifidobacterium longum subspecies infantis - physiology
/ Female
/ Gastrointestinal Microbiome - drug effects
/ Host-Microbiome Interactions
/ Humans
/ Infant
/ Male
/ Microbial Ecology and Evolution
/ Microbial Physiology and Genetics
/ Microbiome Influence on Development
/ Placebos - administration & dosage
/ Probiotics - administration & dosage
