Journal Article

An acetyltransferase effector conserved across Legionella species targets the eukaryotic eIF3 complex to modulate protein translation

Syriste, Lukas, Stogios, Peter J., Patel, Dhruvin, Savchenko, Alexei, Skarina, Tatiana, Patel, Deepak T.

2024

Overview

By translocating effectors inside the eukaryotic host cell, bacteria can modulate host cellular processes in their favor. Legionella species, which includes the pneumonia-causing Legionella pneumophila, encode a widely diverse set of effectors with only a small subset that is conserved across this genus. Here, we demonstrate that one of these conserved effector families, represented by L. pneumophila VipF (Lpg0103), is a tandem Gcn5-related N-acetyltransferase interacting with the K subunit of human eukaryotic initiation factor 3 complex. VipF catalyzes the acetylation of lysine residues on the C-terminal tail of the K subunit, resulting in the suppression of eukaryotic translation initiation factor 3-mediated protein translation in vitro . These new data provide the first insight into the molecular function of this pathogenic factor family common across Legionellae .

• Publisher: American Society for Microbiology
• Subject: Acetylation / Acetyltransferase / Acetyltransferases - metabolism / Bacterial Proteins - metabolism / Catalysis / Chloramphenicol / Coenzyme A / Crystal structure / effectors / Enzymes / Eukaryotic Initiation Factor-3 - metabolism / Genotype & phenotype / Histones / Host-Microbial Interactions / Humans / Intracellular / Legionella / Legionella - genetics / Legionella pneumophila / Legionella pneumophila - genetics / Legionnaire's disease / Legionnaires' Disease / Lysine - metabolism / N-Acetyltransferase / pathogenesis / Peptides / Phylogenetics / Poly-L-lysine / Prokaryotic Initiation Factor-3 - metabolism / Protein Biosynthesis / Proteins / Research Article / RNA polymerase