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An ultrasensitive GRAB sensor for detecting extracellular ATP in vitro and in vivo
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An ultrasensitive GRAB sensor for detecting extracellular ATP in vitro and in vivo
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An ultrasensitive GRAB sensor for detecting extracellular ATP in vitro and in vivo
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Paper
An ultrasensitive GRAB sensor for detecting extracellular ATP in vitro and in vivo
2021
Overview
ATP1.0, with a robust fluorescence response to extracellular ATP when expressed in several cell types. This sensor has sub-second kinetics, ATP affinity in the range of tens of nanomolar, and can be used to localize ATP release with subcellular resolution. Using this sensor, we monitored ATP release under a variety of in vitro and in vivo conditions, including primary hippocampal neurons, a zebrafish model of injury-induced ATP release, and LPS-induced ATP-release events in individual astrocytes in the mouse cortex measured using in vivo two-photon imaging. Thus, the GRABATP1.0 sensor is a sensitive, versatile tool for monitoring ATP release and dynamics under both physiological and pathophysiological conditions. Competing Interest Statement H.W., M.J., and Y.L. have filed patent applications, the value of which may be affected by this publication.
Publisher
Cold Spring Harbor Laboratory Press,Cold Spring Harbor Laboratory
Subject
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