Asset Details
Do you wish to reserve the book?
In vivo modulation of network activity drives the nanoscale reorganisation of axo-axonic synapses at the axon initial segment
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
In vivo modulation of network activity drives the nanoscale reorganisation of axo-axonic synapses at the axon initial segment
Do you wish to request the book?
In vivo modulation of network activity drives the nanoscale reorganisation of axo-axonic synapses at the axon initial segment
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Paper
In vivo modulation of network activity drives the nanoscale reorganisation of axo-axonic synapses at the axon initial segment
2025,2024
Overview
Chemical synapses control their strength through the nanoscale clustering of postsynaptic receptors into sub-synaptic domains (SSDs). Despite their importance in synapse function, the properties and plasticity of these domains are not well understood in vivo, particularly in inhibitory synapses. We used direct Stochastic Optical Resolution Microscopy (dSTORM) to show that Gephyrin, the main inhibitory receptor scaffold protein, is organised into SSDs in vivo, with distinct arrangements depending on their sub-cellular location and presynaptic partner. Furthermore, chronic chemogenetic increases in cortical activity caused a reduction in Gephyrin SSD volume specifically in axo-axonic, but not axo-dendritic, synapses. Functionally, this resulted in a weakening of axo-axonic contacts. We show that the nanoscale arrangement of synapses in the brain is plastic and used to fine-tune synaptic gain in vivo.Competing Interest StatementThe authors have declared no competing interest.Footnotes* Manuscript text and figures have been revised.
Publisher
Cold Spring Harbor Laboratory Press,Cold Spring Harbor Laboratory
Subject
