Asset Details
Do you wish to reserve the book?
TopoStats – a program for automated tracing of biomolecules from AFM images
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
TopoStats – a program for automated tracing of biomolecules from AFM images
Do you wish to request the book?
TopoStats – a program for automated tracing of biomolecules from AFM images
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Paper
TopoStats – a program for automated tracing of biomolecules from AFM images
2021
Overview
Abstract We present TopoStats, a Python toolkit for automated editing and analysis of Atomic Force Microscopy images. The program automates identification and tracing of individual molecules in circular and linear conformations without user input. TopoStats was able to identify and trace a range of molecules within AFM images, finding, on average, 90% of all individual molecules and molecular assemblies within a wide field of view, and without the need for prior processing. DNA minicircles of varying size, DNA origami rings and pore forming proteins were identified and accurately traced with contour lengths of traces typically within 10 nm of the predicted contour length. TopoStats was also able to reliably identify and trace linear and enclosed circular molecules within a mixed population. The program is freely available via GitHub (https://github.com/afm-spm/TopoStats) and is intended to be modified and adapted for use if required. Competing Interest Statement The authors have declared no competing interest. Footnotes * Updated following review * https://github.com/AFM-SPM/TopoStats
Publisher
Cold Spring Harbor Laboratory Press,Cold Spring Harbor Laboratory
Subject
