Journal Article

F. nucleatum targets lncRNA ENO1-IT1 to promote glycolysis and oncogenesis in colorectal cancer

Guo, Fangfang, Yan, Tingting, Yu, TaChung, Liu, Jianjun, Ma, Dan, Xie, Yile, Chen, Ying-Xuan, Qian, Yun, Zhou, Xiang, Liu, Qiang, Zhong, Ming, Chen, Jinxian, Shen, Chaoqin, Zhang, Xinyu, Ma, Xiong, Fang, Jing-Yuan, Peng, Yanshen, Lu, Shi-Yuan, Wang, Cheng, Chen, Haoyan, Hong, Jie, Sun, Tiantian

2021

Overview

ObjectiveMicrobiota disorder promotes chronic inflammation and carcinogenesis. High glycolysis is associated with poor prognosis in patients with colorectal cancer (CRC). However, the potential correlation between the gut microbiota and glucose metabolism is unknown in CRC.Design 18F-FDG (18F-fluorodeoxyglucose) PET (positron emission tomography)/CT image scanning data and microbiota PCR analysis were performed to measure the correlation between metabolic alterations and microbiota disorder in 33 patients with CRC. Multiple colorectal cancer models, metabolic analysis and Seahorse assay were established to assess the role of long non-coding RNA (lncRNA) enolase1-intronic transcript 1 (ENO1-IT1) in Fusobacterium (F.) nucleatum-induced glucose metabolism and colorectal carcinogenesis. RNA immunoprecipitation and chromatin immunoprecipitation sequencing were conducted to identify potential targets of lncRNA ENO1-IT1.ResultsWe have found F. nucleatum abundance correlated with high glucose metabolism in patients with CRC. Furthermore, F. nucleatum supported carcinogenesis via increasing CRC cell glucose metabolism. Mechanistically, F. nucleatum activated lncRNA ENO1-IT1 transcription via upregulating the binding efficiency of transcription factor SP1 to the promoter region of lncRNA ENO1-IT1. Elevated ENO1-IT behaved as a guider modular for KAT7 histone acetyltransferase, specifying the histone modification pattern on its target genes, including ENO1, and consequently altering CRC biological function.Conclusion F. nucleatum and glucose metabolism are mechanistically, biologically and clinically connected to CRC. Targeting ENO1 pathway may be meaningful in treating patients with CRC with elevated F. nucleatum.

• Publisher: BMJ Publishing Group Ltd and British Society of Gastroenterology,BMJ Publishing Group LTD
• Subject: Animals / Biomarkers, Tumor / Cancer / Carcinogenesis / Carcinogenesis - genetics / Cell growth / Chromatin / Colon / Colorectal cancer / Colorectal carcinoma / Colorectal Neoplasms - diagnostic imaging / Colorectal Neoplasms - genetics / DNA-Binding Proteins / Drug resistance / Fluorodeoxyglucose F18 - pharmacokinetics / Fusobacterium Infections - genetics / Fusobacterium nucleatum / Gastrointestinal Microbiome / Gene Expression Regulation, Neoplastic / Glucose metabolism / Glycolysis / Glycolysis - genetics / Histone acetyltransferase / Histone Acetyltransferases / Humans / Immunoprecipitation / intestinal bacteria / Intestinal microflora / Medical imaging / Metabolites / Mice / Microbiota / Non-coding RNA / Patients / Phosphopyruvate Hydratase / Positron emission tomography / Positron Emission Tomography Computed Tomography / Prognosis / Radiopharmaceuticals - pharmacokinetics / RNA, Long Noncoding - genetics / Signal Transduction / Sp1 protein / Tumor Cells, Cultured / Tumor Suppressor Proteins / Tumorigenesis