Journal Article

Sensitive and Specific Detection of Influenza Virus A Subtype H5 with Real-Time PCR

Rossi, J., Cramer, S., Laue, T.

2007

Overview

Avian influenza is a serious threat to both animal and human health. To enable cutting-edge research in this field, we developed a molecular test on the basis of real-time polymerase chain reaction (real-time PCR), which detects influenza virus RNA. The test enables highly sensitive detection of influenza virus A and B strains, including H5N1, and specific identification of influenza A virus H5 subtypes. The kit was tested against a broad panel of influenza A and B subtypes and other respiratory viruses in collaboration with worldwide authoritative laboratories and shows a very high specificity and sensitivity. An internal control verifies RNA extraction as well as real-time PCR success. With this kit, rapid and reliable detection of influenza A and B viruses and identification of H5 subtypes can be achieved.

• Publisher: American Association of Avian Pathologists, Inc
• Subject: accuracy / analytical kits / Animals / avian influenza virus / Cercopithecine herpesvirus 1 / diagnostic techniques / disease detection / genotype / H3N2 subtype influenza A virus / H5N1 / H5N1 subtype influenza A virus / Influenza A virus / Influenza A virus - classification / Influenza A virus - isolation & purification / Influenza B virus - classification / Influenza B virus - isolation & purification / new methods / Orthomyxoviridae / pathogen identification / pathotypes / Polymerase chain reaction / Polymerase Chain Reaction - methods / precision / Reagent Kits, Diagnostic / real-time polymerase chain reaction / real-time RT-PCR / Research Note / Reverse transcriptase polymerase chain reaction / RNA / RRT-PCR / Sensitivity and Specificity / strain differences / strains / test sensitivity / test specificity / vertebrate viruses / Viral RNA / viral RNA extraction / Viruses