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Phenotypic and genotypic characterisation of Brucella strains isolated from cattle in the Gambia
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Phenotypic and genotypic characterisation of Brucella strains isolated from cattle in the Gambia
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Phenotypic and genotypic characterisation of Brucella strains isolated from cattle in the Gambia
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Journal Article
Phenotypic and genotypic characterisation of Brucella strains isolated from cattle in the Gambia
2010
Overview
Thirty-five serum samples and six hygroma fluid samples were collected from sexually mature cattle in one herd with clinical signs of brucellosis (abortion and hygromas) in the Western Region of the Gambia in order to isolate and characterise Brucella species. Information on the sex, age, number of calvings, number of abortions, presence of hygromas, and presence of orchitis was also collected for each animal sampled. Twenty-six (74 per cent) of the serum samples were positive in the rose bengal test and 29 (83 per cent) were positive by indirect ELISA. Three isolates of Brucella, biotyped as Brucella abortus biovar 3, were cultured from six hygroma fluid samples. The multiple locus variable number tandem repeat analysis assay clustered the isolates as B abortus with the same profile for the three isolates, suggesting a common origin of contamination.
Publisher
BMJ Publishing Group Limited,Blackwell Publishing Ltd,British Veterinary Association
Subject
/ Animals
/ Brucella abortus - isolation & purification
/ Brucella melitensis biovar Abortus
/ Brucella/classification/genetics
/ Brucellosis, Bovine - microbiology
/ Cattle
/ enzyme-linked immunosorbent assay
/ Enzyme-Linked Immunosorbent Assay - veterinary
/ Farmers
/ Female
/ Gambia
/ Genotype
/ indirect enzyme-linked immunosorbent assay
/ loci
/ Médecine vétérinaire & santé animale
/ orchitis
/ Serology
/ Sheep
