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Basal condensation of Numb and Pon complex via phase transition during Drosophila neuroblast asymmetric division
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Basal condensation of Numb and Pon complex via phase transition during Drosophila neuroblast asymmetric division
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Basal condensation of Numb and Pon complex via phase transition during Drosophila neuroblast asymmetric division
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Journal Article
Basal condensation of Numb and Pon complex via phase transition during Drosophila neuroblast asymmetric division
2018
Overview
Uneven distribution and local concentration of protein complexes on distinct membrane cortices is a fundamental property in numerous biological processes, including
Drosophila
neuroblast (NB) asymmetric cell divisions and cell polarity in general. In NBs, the cell fate determinant Numb forms a basal crescent together with Pon and is segregated into the basal daughter cell to initiate its differentiation. Here we discover that Numb PTB domain, using two distinct binding surfaces, recognizes repeating motifs within Pon in a previously unrecognized mode. The multivalent Numb-Pon interaction leads to high binding specificity and liquid-liquid phase separation of the complex. Perturbations of the Numb/Pon complex phase transition impair the basal localization of Numb and its subsequent suppression of Notch signaling during NB asymmetric divisions. Such phase-transition-mediated protein condensations on distinct membrane cortices may be a general mechanism for various cell polarity regulatory complexes.
Polarized localization of Numb and Pon in
Drosophila
neuroblasts (NBs) enables their unequal segregation during asymmetric cell divisions. Here, the authors demonstrate liquid-liquid phase separation of Pon and Numb in NBs mediated by multivalent intermolecular interactions is required for their basal condensation.
Publisher
Nature Publishing Group UK,Nature Publishing Group,Nature Portfolio
Subject
/ 13/51
/ 13/89
/ 14/33
/ 38/77
/ 64/24
/ Animals
/ Binding
/ Carrier Proteins - physiology
/ Drosophila melanogaster - embryology
/ Drosophila Proteins - physiology
/ Gene Expression Regulation, Developmental
/ Humanities and Social Sciences
/ Humans
/ Insects
/ Juvenile Hormones - physiology
/ Polarity
/ Science
