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Structural Basis of Transcription Initiation
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Journal Article
/ Pocket
Structural Basis of Transcription Initiation
2012
Overview
During transcription initiation, RNA polymerase (RNAP) binds and unwinds promoter DNA to form an RNAP-promoter open complex. We have determined crystal structures at 2.9 and 3.0 Å resolution of functional transcription initiation complexes comprising Thermus thermophilus RNA polymerase, σ A , and a promoter DNA fragment corresponding to the transcription bubble and downstream double-stranded DNA of the RNAP-promoter open complex. The structures show that σ recognizes the -10 element and discriminator element through interactions that include the unstacking and insertion into pockets of three DNA bases and that RNAP recognizes the -4/+2 region through interactions that include the unstacking and insertion into a pocket of the +2 base. The structures further show that interactions between σ and template-strand single-stranded DNA (ssDNA) preorganize template-strand ssDNA to engage the RNAP active center.
Publisher
American Association for the Advancement of Science,The American Association for the Advancement of Science
Subject
Analytical, structural and metabolic biochemistry
/ Atoms
/ Bacteria
/ Biological and medical sciences
/ Contact
/ Crystals
/ DNA
/ DNA, Single-Stranded - chemistry
/ DNA-Directed RNA Polymerases - chemistry
/ Fundamental and applied biological sciences. Psychology
/ Gene Expression Regulation, Bacterial
/ Genes
/ Molecular and cellular biology
/ proteins
/ RNA
/ Thermus thermophilus - enzymology
