Asset Details
Do you wish to reserve the book?
Autocrine IGF-1 Action in Adipocytes Controls Systemic IGF-1 Concentrations and Growth
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Autocrine IGF-1 Action in Adipocytes Controls Systemic IGF-1 Concentrations and Growth
Do you wish to request the book?
Autocrine IGF-1 Action in Adipocytes Controls Systemic IGF-1 Concentrations and Growth
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Autocrine IGF-1 Action in Adipocytes Controls Systemic IGF-1 Concentrations and Growth
2008
Overview
Autocrine IGF-1 Action in Adipocytes Controls Systemic IGF-1 Concentrations and Growth
Nora Klöting 1 ,
Linda Koch 2 ,
Thomas Wunderlich 2 ,
Matthias Kern 1 ,
Karen Ruschke 1 ,
Wilhelm Krone 3 ,
Jens C. Brüning 2 and
Matthias Blüher 1 3 4
1 Department of Medicine, University of Leipzig, Leipzig, Germany
2 Department of Mouse Genetics and Metabolism, Institute for Genetics, University of Cologne and Center of Molecular Medicine
Cologne, Cologne, Germany
3 Department of Internal Medicine II, University of Cologne and Center of Molecular Medicine Cologne, Cologne, Germany
4 Interdisciplinary Center for Clinical Research (IZKF), Leipzig, Germany
Corresponding authors: Jens C. Brüning, jens.bruening{at}uni-koeln.de ; Matthias Blüher, bluma{at}medizin.uni-leipzig.de
Abstract
OBJECTIVE— IGF-1 and the IGF-1 receptor (IGF-1R) have been implicated in the regulation of adipocyte differentiation and lipid accumulation
in vitro.
RESEARCH DESIGN AND METHODS— To investigate the role of IGF-1 receptor in vivo, we have inactivated the Igf-1r gene in adipose tissue (IGF-1R aP2Cre mice) using conditional gene targeting strategies.
RESULTS— Conditional IGF-1R inactivation resulted in increased adipose tissue mass with a predominantly increased lipid accumulation
in epigonadal fat pads. However, insulin-stimulated glucose uptake into adipocytes was unaffected by the deletion of the IGF-1R.
Surprisingly, IGF-1R aP2Cre mice exhibited markedly increased somatic growth in the presence of elevated IGF-1 serum concentrations, and IGF-1 mRNA expression
was significantly increased in liver and adipose tissue. IGF-1 stimulation of wild-type adipocytes significantly decreased
IGF-1 mRNA expression, whereas the opposite effect was observed in IGF-1R–deficient adipocytes.
CONCLUSIONS— IGF-1R signaling in adipocytes does not appear to be crucial for the development and differentiation of adipose tissue in
vivo, but we identified a negative IGF-1R–mediated feedback mechanism of IGF-1 on its own gene expression in adipocytes, indicating
an unexpected role for adipose tissue IGF-1 signaling in the regulation of IGF-1 serum concentrations in control of somatic
growth.
Footnotes
Published ahead of print at http://diabetes.diabetesjournals.org on 28 April 2008.
N.K. and L.K. contributed equally to this work.
Readers may use this article as long as the work is properly cited, the use is educational and not for profit, and the work
is not altered. See http://creativecommons.org/licenses/by-nc-nd/3.0/ for details.
The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore
be hereby marked “advertisement” in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
Accepted April 22, 2008.
Received October 29, 2007.
DIABETES
Publisher
American Diabetes Association
Subject
/ Animals
/ Biological and medical sciences
/ Body fat
/ Diabetes. Impaired glucose tolerance
/ Endocrine pancreas. Apud cells (diseases)
/ Etiopathogenesis. Screening. Investigations. Target tissue resistance
/ Growth
/ Insulin
/ Insulin-like growth factor 1
/ Insulin-Like Growth Factor Binding Protein 3 - blood
/ Insulin-like growth factor I
/ Insulin-Like Growth Factor I - genetics
/ Insulin-Like Growth Factor I - metabolism
/ Insulin-Like Growth Factor I - physiology
/ Kinases
/ Mice
/ Receptor, IGF Type 1 - genetics
/ Receptor, IGF Type 1 - physiology
