Asset Details
Do you wish to reserve the book?
Specific detection of Muscovy duck parvovirus infection by TaqMan-based real-time PCR assay
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Specific detection of Muscovy duck parvovirus infection by TaqMan-based real-time PCR assay
Do you wish to request the book?
Specific detection of Muscovy duck parvovirus infection by TaqMan-based real-time PCR assay
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Specific detection of Muscovy duck parvovirus infection by TaqMan-based real-time PCR assay
2018
Overview
Background
Muscovy duck parvovirus (MDPV) causes high mortality and morbidity in Muscovy ducks, with the pathogenesis of the virus still unknown in many respects. Specific MDPV detection is often rife with false positive results because of high identity at the genomic nucleotide level and antigenic similarity with goose parvovirus (GPV). The objective of this study was to develop a sensitive, highly specific, and repeatable TaqMan-based real-time PCR (qPCR) assay for facilitating the molecular detection of MDPV.
Results
The specific primers and probe were designed based on the conserved regions within MDPVs, but there was a variation in GPVs of the nonstructural (NS) genes after genetic comparison. After the optimization of qPCR conditions, the detection limit of this qPCR assay was 29.7 copies/μl. The assay was highly specific for the detection of MDPV, and no cross-reactivity was observed with other non-targeted duck-derived pathogens. Intra- and inter-assay variability was less than 2.21%, means a high degree of repeatability. The diagnostic applicability of the qPCR assay was proven that MDPV-positive can be found in cloacal swabs samples, Muscovy duck embryos and newly hatched Muscovy ducklings.
Conclusions
Our data provided incidents that MDPV could be possible vertically transmitted from breeder Muscovy ducks to Muscovy ducklings. The developed qPCR assay in the study could be a reliable and specific tool for epidemiological surveillance and pathogenesis studies of MDPV.
Publisher
BioMed Central,BioMed Central Ltd,Springer Nature B.V,BMC
Subject
/ Animals
/ Anseriform dependoparvovirus 1
/ cloaca
/ DNA
/ Ducks
/ E coli
/ Embryo, Nonmammalian - virology
/ Embryos
/ Genes
/ Genomes
/ genomics
/ Infectious Disease Transmission, Vertical - veterinary
/ MDPV
/ Medicine
/ Muscovy
/ NS gene
/ Parvoviridae Infections - transmission
/ Parvoviridae Infections - veterinary
/ Parvoviridae Infections - virology
/ Parvovirus - isolation & purification
/ Primers
/ Proteins
/ quantitative polymerase chain reaction
/ Real-Time Polymerase Chain Reaction - methods
/ Real-Time Polymerase Chain Reaction - veterinary
/ TaqMan-based real-time PCR assay
/ Taxonomy
/ Veterinary Medicine/Veterinary Science
/ Virology
/ Viruses
/ Zoology
