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Live-imaging fluorescent proteins in mouse embryos: multi-dimensional, multi-spectral perspectives
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Live-imaging fluorescent proteins in mouse embryos: multi-dimensional, multi-spectral perspectives
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Journal Article
Live-imaging fluorescent proteins in mouse embryos: multi-dimensional, multi-spectral perspectives
2009
Overview
Microscopy has always been an obligate tool in the field of developmental biology, a goal of which is to elucidate the essential cellular and molecular interactions that coordinate the specification of different cell types and the establishment of body plans. The 2008 Nobel Prize in chemistry was awarded ‘for the discovery and development of the green fluorescent protein, GFP’ in recognition that the discovery of genetically encoded fluorescent proteins (FPs) has spearheaded a revolution in applications for imaging of live cells. With the development of more-sophisticated imaging technology and availability of FPs with different spectral characteristics, dynamic processes can now be live-imaged at high resolution in situ in embryos. Here, we review some recent advances in this rapidly evolving field as applied to live-imaging capabilities in the mouse, the most genetically tractable mammalian model organism for embryologists.
Publisher
Elsevier Ltd,[London]: Elsevier Science,Cell Press,Elsevier Limited
Subject
/ Biological and medical sciences
/ Cloning
/ Embryo, Mammalian - cytology
/ Embryo, Mammalian - metabolism
/ Embryos
/ Fluorescent Antibody Technique - methods
/ Fluorescent Antibody Technique - trends
/ Fundamental and applied biological sciences. Psychology
/ Luminescent Proteins - metabolism
/ mice
/ Microscopy, Fluorescence - methods
/ Microscopy, Fluorescence - trends
/ Proteins
/ Studies
