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Neuronal morphology and projection analysis by multispectral tracing in densely labeled mouse brain
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Neuronal morphology and projection analysis by multispectral tracing in densely labeled mouse brain
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Paper
Neuronal morphology and projection analysis by multispectral tracing in densely labeled mouse brain
2018
Overview
Accurate and complete neuronal wiring diagrams are necessary for understanding brain function at many scales from long-range interregional projections to microcircuits. Traditionally, light microscopy-based anatomical reconstructions use monochromatic labeling and therefore necessitate sparse labeling to eliminate tracing ambiguity between intermingled neurons. Consequently, our knowledge of neuronal morphology has largely been based on averaged estimations across many samples. Recently developed second-generation Brainbow tools promise to circumvent this limitation by revealing fine anatomical details of many unambiguously identifiable neurons in densely labeled samples. Yet, a means to quantify and analyze the information is currently lacking. Therefore, we developed nTracer, an ImageJ plug-in capable of rapidly and accurately reconstructing whole-cell morphology of large neuronal populations in densely labeled brains. Footnotes * Title change and added author
Publisher
Cold Spring Harbor Laboratory Press,Cold Spring Harbor Laboratory
Subject
