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The primary transcriptome of the major human pathogen Helicobacter pylori
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The primary transcriptome of the major human pathogen Helicobacter pylori
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Journal Article
The primary transcriptome of the major human pathogen Helicobacter pylori
2010
Overview
Genome sequencing of
Helicobacter pylori
has revealed the potential proteins and genetic diversity of this prevalent human pathogen, yet little is known about its transcriptional organization and noncoding RNA output. Massively parallel cDNA sequencing (RNA-seq) has been revolutionizing global transcriptomic analysis. Here, using a novel differential approach (dRNA-seq) selective for the 5′ end of primary transcripts, we present a genome-wide map of
H. pylori
transcriptional start sites and operons. We discovered hundreds of transcriptional start sites within operons, and opposite to annotated genes, indicating that complexity of gene expression from the small
H. pylori
genome is increased by uncoupling of polycistrons and by genome-wide antisense transcription. We also discovered an unexpected number of ∼60 small RNAs including the ε-subdivision counterpart of the regulatory 6S RNA and associated RNA products, and potential regulators of
cis
- and
trans
-encoded target messenger RNAs. Our approach establishes a paradigm for mapping and annotating the primary transcriptomes of many living species.
The
Helicobacter pylori
transcriptome
The bacterium
Helicobacter pylori
infects about half the human population, thriving in the acid conditions of the stomach. Most carriers are asymptomatic, but some suffer inflammation, ulcers and gastric cancer. Now, using a novel approach that selects for the 5' ends of primary transcripts, the 'primary transcriptome' — predominantly the unprocessed messenger RNAs and small non-coding RNAs — has been determined for
H. pylori
in a variety of growth conditions. With the genome sequence and protein interactome previously published, this work provides the third global reference data set for the widely used
Helicobacter
strain 26695.
The transcriptome of
Helicobacter pylori
, an important human pathogen involved in gastric ulcers and cancer, is presented. The approach establishes a model for mapping and annotating the primary transcriptomes of many living species.
Publisher
Nature Publishing Group UK,Nature Publishing Group
Subject
5' Untranslated Regions - genetics
/ Analysis
/ Bacteria
/ Biological and medical sciences
/ E coli
/ Fundamental and applied biological sciences. Psychology
/ Genetics
/ Genome, Bacterial - genetics
/ Helicobacter Infections - microbiology
/ Helicobacter pylori - genetics
/ Humanities and Social Sciences
/ Humans
/ Proteins
/ Science
