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Development and Validation of RP-HPLC Method for Simultaneous Estimation of Metformin and Miglitol in Bulk and Dosage Form
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Development and Validation of RP-HPLC Method for Simultaneous Estimation of Metformin and Miglitol in Bulk and Dosage Form
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Development and Validation of RP-HPLC Method for Simultaneous Estimation of Metformin and Miglitol in Bulk and Dosage Form
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Journal Article
Development and Validation of RP-HPLC Method for Simultaneous Estimation of Metformin and Miglitol in Bulk and Dosage Form
2017
Overview
[...]the method was found to be simple, accurate, precise, economic and reproducible. [...]the method can be used for the routine and stability analysis in various pharmaceutical industries in bulk drugs and formulations. Literature survey revealed that several analytical and bio-analytical methods for its estimation using RP-HPLC with UV detection, HPLC- electrospray tandem mass spectrometry, LC-MS, liquid chromatography with atmospheric pressure chemical ionization tandem mass spectrometry and RP -HPLC method [15-19]. Linearity and Range: The data obtained in the calibration experiments when subjected to linear regression analysis showed a linear relationship between peak areas and concentrations in the range 10-50 g/ml for MET and 1-5 g/ml for MIG [Table 3 and 4] depict the calibration data of MET and MIG.The respective linear equation for Metformin was y = 103.0x + 123.0 and Miglitol equation y = 85.97 x-3.638.
Publisher
Athena Information Solutions Pvt. Ltd
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