Journal Article

Specific presynaptic functions require distinct Drosophila Cav2 splice isoforms

Ryglewski, Stefanie, Arian, Jashar, Kobler, Oliver, Rickert, Christof, Strauß, Julia, Gottschalk, Daniel, Deneke, Lea, Duch, Carsten, Bell, Christopher, Kern, Hanna, Heine, Martin, Kilo, Lukas

2025

Overview

At many vertebrate synapses, presynaptic functions are tuned by expression of different Ca v 2 channels. Most invertebrate genomes contain only one Ca v 2 gene. The Drosophila Ca v 2 homolog, cacophony (cac), induces synaptic vesicle release at presynaptic active zones (AZs). We hypothesize that Drosophila cac functional diversity is enhanced by two mutually exclusive exon pairs that are not conserved in vertebrates, one in the voltage sensor and one in the loop binding Ca β and G βγ subunits. We find that alternative splicing in the voltage sensor affects channel activation voltage. Only the isoform with the higher activation voltage localizes to AZs at the glutamatergic Drosophila larval neuromuscular junction and is imperative for normal synapse function. By contrast, alternative splicing at the other alternative exon pair tunes multiple aspects of presynaptic function. While expression of one exon yields normal transmission, expression of the other reduces channel number in the AZ and thus release probability. This also abolishes presynaptic homeostatic plasticity. Moreover, reduced channel number affects short-term plasticity, which is rescued by increasing the external calcium concentration to match release probability to control. In sum, in Drosophila alternative splicing provides a mechanism to regulate different aspects of presynaptic functions with only one Ca v 2 gene.

• Publisher: eLife Sciences Publications Ltd
• Subject: alternative splicing / cacophony / Cav2 / synapse / synaptic function