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TaqMan-based quantitative real-time polymerase chain reaction assay to detect porcine circovirus-like virus

by Yu, Z.R. , Chen, Z. , Tu, J. , Cheng, F.Y. , Wang, Z.Y. , Liu, H. , Qi, K.Z. , Shao, Y. , Song, X.J. , Zhang, Y.

in Cross-reactivity / Diarrhea / Epidemiology / Feces / Genetic testing / Hogs / Infections / Orf4 gene / Polymerase chain reaction / Reproducibility / Thermal cycling / Vaccines / Viruses

2024

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TaqMan-based quantitative real-time polymerase chain reaction assay to detect porcine circovirus-like virus

by Yu, Z.R. , Chen, Z. , Tu, J. , Cheng, F.Y. , Wang, Z.Y. , Liu, H. , Qi, K.Z. , Shao, Y. , Song, X.J. , Zhang, Y.

in Cross-reactivity / Diarrhea / Epidemiology / Feces / Genetic testing / Hogs / Infections / Orf4 gene / Polymerase chain reaction / Reproducibility / Thermal cycling / Vaccines / Viruses

2024

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TaqMan-based quantitative real-time polymerase chain reaction assay to detect porcine circovirus-like virus

by Yu, Z.R. , Chen, Z. , Tu, J. , Cheng, F.Y. , Wang, Z.Y. , Liu, H. , Qi, K.Z. , Shao, Y. , Song, X.J. , Zhang, Y.

in Cross-reactivity / Diarrhea / Epidemiology / Feces / Genetic testing / Hogs / Infections / Orf4 gene / Polymerase chain reaction / Reproducibility / Thermal cycling / Vaccines / Viruses

2024

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Journal Article

TaqMan-based quantitative real-time polymerase chain reaction assay to detect porcine circovirus-like virus

Yu, Z.R.,

Chen, Z.,

Tu, J.,

Cheng, F.Y.,

Wang, Z.Y.,

Liu, H.,

Qi, K.Z.,

Shao, Y.,

Song, X.J.,

Zhang, Y.

2024

Overview

The aim of this study was to develop a rapid, sensitive and highly specific TaqMan quantitative real-time polymerase chain reaction PCR (qPCR) assay for porcine circovirus-like virus (PCLV). The primers and probe were designed based on the conserved regions of the PCLV ORF4 gene. The assay has a good detection performance (y = -3.3257x + 1.482, R2 = 0.9905), with a limit of detection of 10 copies, which was 100 times more sensitive than conventional PCR (cPCR). No cross-reactivity was observed with other common viruses. The intra- and inter-assay coefficients of variation were less than 1.25%. 36 fecal samples were analyzed using this method, detecting a positivity rate of 8.33% (3/36) that was higher than the cPCR detected. In summary, the established assay for PCLV detection has high specificity, sensitivity, and reproducibility and can be used as a tool for clinical diagnosis and epidemiological investigation.

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Publisher

Polish Academy of Sciences, Committee of Veterinary Sciences

Subject

/ Feces

/ Hogs