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Molecular Study of the Two Species of senna L. in Iraq
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Molecular Study of the Two Species of senna L. in Iraq
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Journal Article
Molecular Study of the Two Species of senna L. in Iraq
2025
Overview
In this study, genetic information was obtained as evidence of proper identification to detect the divergence between the species belonging to the genus Senna.L, namely Senna alata (Linnaeus) Roxb and Senna didymobotrya (fresen) of the legume family Leguminosae (Fabaceae). Field work was carried out during the growing season of 10/1/2023. DNA was extracted from fresh leaves of the two plant species. We used the polymerase chain reaction - genomic reaction of the chloroplast DNA. The matK region of the chloroplast DNA was amplified using primers and the nucleotide sequences of each species were obtained. The results showed that the molecular weight of the band size for the two species was 902 Pb and according to the barcode classification, the entire genome is considered as a super barcode for the two samples under study. The matK gene sequence was obtained from the GenBank and used for analysis. The phylogenetic tree was designed to identify the ideal regions that can be used to determine the relationships between the two species.It was compared with the global plant sequences closest to the two species and the sequence was registered in the gene bank. The genetic tree showed that the species Senna didymobotrya deposited under the serial number MN243366.1 is the closest global species to the plant Senna didymobotrya under study in Iraq and is linked by up to 100% about the rest of the species. The research also showed that the most global plant related to the species Senna alata is the plant Senna alata registered under the serial number (LC385932.1) at a rate of 99%.
Publisher
IOP Publishing
Subject
