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BoEXPA2 Functions in Brassica oleracea var. acephala DC Seed Germination
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BoEXPA2 Functions in Brassica oleracea var. acephala DC Seed Germination
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Journal Article
BoEXPA2 Functions in Brassica oleracea var. acephala DC Seed Germination
2024
Overview
Expansins are cell wall proteins that promote cell wall relaxation by inducing pH-dependent cell wall elongation and stress relaxation. The activities of expansins are essential in a series of physiological development processes in higher plants, including seed germination. Here, we identified a kale (
Brassica oleracea
var. acephala DC.) expansin gene,
BoEXPA2
, that is only expressed in germinated seeds. A mutant of this gene showed delayed germination, confirming that
BoEXPA2
helps regulate seed germination. Exogenous gibberellins (GA) increased the expression level of
BoEXPA2
during seed germination, while abscisic acid (ABA) had no effect on
BoEXPA2
expression. When subjected to stress, the germination rate of seeds with inhibited
BoEXPA2
expression was significantly reduced relative to that of wild-type seeds, while the germination rate was significantly increased in
BoEXPA2
overexpression lines. Under drought and salt stress, the activities of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) and the content of Malondialdehyde (MDA) increased in seeds of all three genotypes, but the activities of SOD, POD, and CAT were far higher in the seeds of
BoEXPA2
overexpression strains than those of the control and inhibited expression strains, and the activities of MDA were lower than those of the control and inhibition expression strains. In conclusion, our results indicate that
BoEXPA2
participates in GA-mediated seed germination and promotes tolerance to salt stress and osmotic stress in kale.
