Asset Details
Do you wish to reserve the book?
Beyond Cultivation: Combining Culture-Dependent and Culture-Independent Techniques to Identify Bacteria Involved in Paint Spoilage
Hey, we have placed the reservation for you!
By the way, why not check out events that you can attend while you pick your title.
Oops! Something went wrong.
Looks like we were not able to place the reservation. Kindly try again later.
Are you sure you want to remove the book from the shelf?
Beyond Cultivation: Combining Culture-Dependent and Culture-Independent Techniques to Identify Bacteria Involved in Paint Spoilage
Do you wish to request the book?
Beyond Cultivation: Combining Culture-Dependent and Culture-Independent Techniques to Identify Bacteria Involved in Paint Spoilage
Please be aware that the book you have requested cannot be checked out. If you would like to checkout this book, you can reserve another copy
We have requested the book for you!
Your request is successful and it will be processed during the Library working hours. Please check the status of your request in My Requests.
Oops! Something went wrong.
Looks like we were not able to place your request. Kindly try again later.
Journal Article
Beyond Cultivation: Combining Culture-Dependent and Culture-Independent Techniques to Identify Bacteria Involved in Paint Spoilage
2023
Overview
Due to globally increasing problems concerning biodeterioration of paints, it is worthwhile to enhance the determination of colony forming units (CFU) as a gold standard method via more rapid and culture-independent techniques. Here, we combined traditional culture-dependent techniques with subsequent sequencing, quantitative qPCR, and a serial quantification method (most probable number; MPN) to detect paint degrading bacteria in general and sulfate-reducing bacteria (SRB) in particular. During our investigation of three water-based paints that showed visible contamination, we found high bacterial counts of up to 107 CFU mL−1. Subsequent sequencing allowed the identification of common paint degraders including Bacillus sp. and Pseudomonas sp., but less frequently detected bacteria such as Rhodococcus sp. and Delftia spp. were also found to be present. MPN, as well as dsrA-targeted qPCR to detect SRB, only showed positive results for two out of three samples. These results coincided with the inherent physicochemical properties of the paints offering suitable conditions for microbial growth or not. The MPN method can be used for a diversity of aerobic and anaerobic bacteria and is rapid and reproducible. A combination of culture-independent techniques such as qPCR or NGS can help to fully elucidate the bacterial diversity in spoiled paint by also recovering anaerobic and unculturable ones.
