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Cross-species transfer of SSR markers in Setaria sphacelata and Trichloris crinita sp
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Cross-species transfer of SSR markers in Setaria sphacelata and Trichloris crinita sp
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Cross-species transfer of SSR markers in Setaria sphacelata and Trichloris crinita sp
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Journal Article
Cross-species transfer of SSR markers in Setaria sphacelata and Trichloris crinita sp
2019
Overview
Setaria sphacelata and Trichloris crinita are subtropical forage species that are important for livestock breeding in Argentina. Genomic information is scarce for these species, and there are no molecular markers designed for them; this limits the development of genetic improvement programs. We performed a cross-species transfer of SSR markers from several Poaceae species. In S. sphacelata, 8 SSR markers were transferred from Setaria italica (40% transfer rate), exhibiting 83% polymorphism. Kazungula, Splenda and Narok cultivars were genetically differentiated and the experimental material “Selección INTA” was separated from Narok, from which it was derived. For T. crinita, 19 microsatellites were transferred from 5 Poaceae species (7.3% transfer rate), with 69% polymorphism. The results obtained in this study show the potential of the transferred SSR markers for assessing genetic variation and for expanding the genetic resources available for these species.
Publisher
Universidad Nacional de Colombia, Facultad de Agronomía,Centro Editorial of Facultad de Ciencias Agrarias, Universidad Nacional de Colombia
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